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פותח על ידי קלירמאש פתרונות בע"מ -
Extended phylogenetic analysis of a new Israeli isolate of Brevicoryne brassicae virus (BrBV-IL) suggests taxonomic revision of the genus Iflavirus
Year:
2016
Source of publication :
Virology Journal
Authors :
דומברובסקי, אביב
;
.
לוריא, נטע
;
.
לכמן, עודד
;
.
סלע, נעה
;
.
ריינגולד, ויקטוריה
;
.
Volume :
13
Co-Authors:
Luria, N., Department of Plant Pathology, ARO, Volcani Center, Bet Dagan, Israel
Reingold, V., Department of Plant Pathology, ARO, Volcani Center, Bet Dagan, Israel
Lachman, O., Department of Plant Pathology, ARO, Volcani Center, Bet Dagan, Israel
Sela, N., Department of Plant Pathology, ARO, Volcani Center, Bet Dagan, Israel
Dombrovsky, A., Department of Plant Pathology, ARO, Volcani Center, Bet Dagan, Israel
Facilitators :
From page:
0
To page:
0
(
Total pages:
1
)
Abstract:
Background: Brevicoryne brassicae virus (BrBV) is a positive-strand genomic RNA virus which is unassigned tentative member of the genus Iflavirus. BrBv was first identified and characterized in the late 90's in the cabbage aphid in the United Kingdom (UK) (J Gen Virol 88:2590-2595, 2007) and was fully sequenced, using random amplification of encapsidated RNA. No other reports have been published demonstrating detection of this virus outside the UK. Findings: A new isolate of the cabbage aphid virus Brevicoryne brassicae virus was identified from Brevicoryne brassicae aphids growing on wild mustard plants (Sinapis arvensis) in northern Israel. The virus genome was partially assembled from purified siRNA using the Illumina MiSeq Sequencing System with limited success. Combining classical viral RNA purification and RT-PCR amplification followed by traditional Sanger sequencing enabled obtaining the complete genomic sequence. The Israeli strain of BrBV shared 95 % nucleotide sequence identity with the BrBV found in the United Kingdom. Conclusions: The detection of BrBV in Israel indicates a broader geographical distribution of the virus". © 2016 Luria et al.
Note:
Related Files :
Aphid-infecting virus
gene sequence
Israel
reverse transcription polymerase chain reaction
RNA purification
virus gene
עוד תגיות
תוכן קשור
More details
DOI :
10.1186/s12985-016-0500-z
Article number:
500
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
26496
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:23
You may also be interested in
Scientific Publication
Extended phylogenetic analysis of a new Israeli isolate of Brevicoryne brassicae virus (BrBV-IL) suggests taxonomic revision of the genus Iflavirus
13
Luria, N., Department of Plant Pathology, ARO, Volcani Center, Bet Dagan, Israel
Reingold, V., Department of Plant Pathology, ARO, Volcani Center, Bet Dagan, Israel
Lachman, O., Department of Plant Pathology, ARO, Volcani Center, Bet Dagan, Israel
Sela, N., Department of Plant Pathology, ARO, Volcani Center, Bet Dagan, Israel
Dombrovsky, A., Department of Plant Pathology, ARO, Volcani Center, Bet Dagan, Israel
Extended phylogenetic analysis of a new Israeli isolate of Brevicoryne brassicae virus (BrBV-IL) suggests taxonomic revision of the genus Iflavirus
Background: Brevicoryne brassicae virus (BrBV) is a positive-strand genomic RNA virus which is unassigned tentative member of the genus Iflavirus. BrBv was first identified and characterized in the late 90's in the cabbage aphid in the United Kingdom (UK) (J Gen Virol 88:2590-2595, 2007) and was fully sequenced, using random amplification of encapsidated RNA. No other reports have been published demonstrating detection of this virus outside the UK. Findings: A new isolate of the cabbage aphid virus Brevicoryne brassicae virus was identified from Brevicoryne brassicae aphids growing on wild mustard plants (Sinapis arvensis) in northern Israel. The virus genome was partially assembled from purified siRNA using the Illumina MiSeq Sequencing System with limited success. Combining classical viral RNA purification and RT-PCR amplification followed by traditional Sanger sequencing enabled obtaining the complete genomic sequence. The Israeli strain of BrBV shared 95 % nucleotide sequence identity with the BrBV found in the United Kingdom. Conclusions: The detection of BrBV in Israel indicates a broader geographical distribution of the virus". © 2016 Luria et al.
Scientific Publication
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