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פותח על ידי קלירמאש פתרונות בע"מ -
Flower development in garlic: The ups and downs of gaLFY expression
Year:
2011
Source of publication :
Planta
Authors :
דוד-שוורץ, רקפת
;
.
פליישמן, משה
;
.
קמנצקי, רינה
;
.
Volume :
233
Co-Authors:
Neta, R., The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, The Robert H. Smith Institute of Plant Science and Genetics in Agriculture, 76100 Rehovot, Israel
David-Schwartz, R., Institute of Plant Sciences, ARO, The Volcani Center, 50250 Bet Dagan, Israel, The Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat Gan, Israel
Peretz, Y., The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, The Robert H. Smith Institute of Plant Science and Genetics in Agriculture, 76100 Rehovot, Israel
Sela, I., The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, The Robert H. Smith Institute of Plant Science and Genetics in Agriculture, 76100 Rehovot, Israel
Rabinowitch, H.D., The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, The Robert H. Smith Institute of Plant Science and Genetics in Agriculture, 76100 Rehovot, Israel
Flaishman, M., Institute of Plant Sciences, ARO, The Volcani Center, 50250 Bet Dagan, Israel
Kamenetsky, R., Institute of Plant Sciences, ARO, The Volcani Center, 50250 Bet Dagan, Israel
Facilitators :
From page:
1063
To page:
1072
(
Total pages:
10
)
Abstract:
The lack of sexual processes prohibits genetic studies and conventional breeding in commercial cultivars of garlic. Recent restoration of garlic flowering ability by environmental manipulations has opened new avenues for physiological and genetic studies. The LEAFY homologue gaLFY has been shown to be involved in the floral development, while two alternatively spliced gaLFY transcripts are expressed in flowering genotypes. In the present work, quantitative real-time PCR and two techniques of RNA in situ hybridization were employed to analyze spatiotemporal expression patterns of the gaLFY during consequent stages of the garlic reproductive process. Temporal accumulation of gaLFY is strongly associated with reproductive organs, significantly increased during florogenesis and gametogenesis, and is down-regulated in the vegetative meristems and topsets in the inflorescence. The two alternative transcripts of the gene show different expression patterns: a high level of the long gaLFY transcript coincided only with floral transition, while further up-regulation of this gene in the reproductive organs is associated mainly with the short gaLFY transcript. It is concluded that gaLFY is involved at different stages of the sexual reproduction of garlic. These new insights broaden our basic understanding of flower biology of garlic and help to establish conventional and molecular breeding systems for this important crop. © 2011 Springer-Verlag.
Note:
Related Files :
עוד תגיות
תוכן קשור
More details
DOI :
10.1007/s00425-011-1361-8
Article number:
0
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
26540
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:23
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Scientific Publication
Flower development in garlic: The ups and downs of gaLFY expression
233
Neta, R., The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, The Robert H. Smith Institute of Plant Science and Genetics in Agriculture, 76100 Rehovot, Israel
David-Schwartz, R., Institute of Plant Sciences, ARO, The Volcani Center, 50250 Bet Dagan, Israel, The Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat Gan, Israel
Peretz, Y., The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, The Robert H. Smith Institute of Plant Science and Genetics in Agriculture, 76100 Rehovot, Israel
Sela, I., The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, The Robert H. Smith Institute of Plant Science and Genetics in Agriculture, 76100 Rehovot, Israel
Rabinowitch, H.D., The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, The Robert H. Smith Institute of Plant Science and Genetics in Agriculture, 76100 Rehovot, Israel
Flaishman, M., Institute of Plant Sciences, ARO, The Volcani Center, 50250 Bet Dagan, Israel
Kamenetsky, R., Institute of Plant Sciences, ARO, The Volcani Center, 50250 Bet Dagan, Israel
Flower development in garlic: The ups and downs of gaLFY expression
The lack of sexual processes prohibits genetic studies and conventional breeding in commercial cultivars of garlic. Recent restoration of garlic flowering ability by environmental manipulations has opened new avenues for physiological and genetic studies. The LEAFY homologue gaLFY has been shown to be involved in the floral development, while two alternatively spliced gaLFY transcripts are expressed in flowering genotypes. In the present work, quantitative real-time PCR and two techniques of RNA in situ hybridization were employed to analyze spatiotemporal expression patterns of the gaLFY during consequent stages of the garlic reproductive process. Temporal accumulation of gaLFY is strongly associated with reproductive organs, significantly increased during florogenesis and gametogenesis, and is down-regulated in the vegetative meristems and topsets in the inflorescence. The two alternative transcripts of the gene show different expression patterns: a high level of the long gaLFY transcript coincided only with floral transition, while further up-regulation of this gene in the reproductive organs is associated mainly with the short gaLFY transcript. It is concluded that gaLFY is involved at different stages of the sexual reproduction of garlic. These new insights broaden our basic understanding of flower biology of garlic and help to establish conventional and molecular breeding systems for this important crop. © 2011 Springer-Verlag.
Scientific Publication
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