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פותח על ידי קלירמאש פתרונות בע"מ -
Transcriptome analysis of stress tolerance in entomopathogenic nematodes of the genus Steinernema
Year:
2016
Authors :
גלזר, איתמר
;
.
דורון-פייגנבאום, עדי
;
.
יערי, מור
;
.
סלמה, ליאורה
;
.
קולטאי, חננית
;
.
Volume :
46
Co-Authors:
Yaari, M., Department of Entomology and Nematology, Agricultural Research Organization, The Volcani Center, P.O. Box 6, Bet Dagan, Israel
Doron-Faigenboim, A., Department of Ornamental Horticulture, Agricultural Research Organization, Israel
Koltai, H., Department of Ornamental Horticulture, Agricultural Research Organization, Israel
Salame, L., Department of Entomology and Nematology, Agricultural Research Organization, The Volcani Center, P.O. Box 6, Bet Dagan, Israel
Glazer, I., Department of Entomology and Nematology, Agricultural Research Organization, The Volcani Center, P.O. Box 6, Bet Dagan, Israel
Facilitators :
From page:
83
To page:
95
(
Total pages:
13
)
Abstract:
Entomopathogenic nematodes of the genus Steinernema are effective biological control agents. The infective stage of these parasites can withstand environmental stresses such as desiccation and heat, but the molecular and physiological mechanisms involved in this tolerance are poorly understood. We used 454 pyrosequencing to analyse transcriptome expression in Steinernema spp. that differ in their tolerance to stress. We compared these species, following heat and desiccation treatments, with their non-stressed counterparts. More than 98% of the transcripts found matched homologous sequences in the UniRef90 database, mostly nematode genes (85%). Among those, 60.8% aligned to the vertebrate parasites including Ascaris suum, Loa loa, and Brugia malayi, 23.3% aligned to bacteriovores, mostly from the genus Caenorhabditis, and 1% aligned to EPNs. Analysing gene expression patterns of the stress response showed a large fraction of down-regulated genes in the desiccation-tolerant nematode Steinernema riobrave, whereas a larger fraction of the genes in the susceptible Steinernema feltiae Carmiel and Gvulot strains were up-regulated. We further compared metabolic pathways and the expression of specific stress-related genes. In the more tolerant nematode, more genes were down-regulated whereas in the less tolerant strains, more genes were up-regulated. This phenomenon warrants further exploration of the mechanism governing induction of the down-regulation process. The present study revealed many genes and metabolic cycles that are differentially expressed in the stressed nematodes. Some of those are well known in other nematodes or anhydrobiotic organisms, but several are new and should be further investigated for their involvement in desiccation and heat tolerance. Our data establish a foundation for further exploration of stress tolerance in entomopathogenic nematodes and, in the long term, for improving their ability to withstand suboptimal environmental conditions. © 2015 Australian Society for Parasitology Inc..
Note:
Related Files :
Environmental stress
gene expression
heat stress
heat tolerance
Nematoda
Parasite
stress
עוד תגיות
תוכן קשור
More details
DOI :
10.1016/j.ijpara.2015.08.011
Article number:
0
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:

Itamar's own PDF.

ID:
26557
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:23
You may also be interested in
Scientific Publication
Transcriptome analysis of stress tolerance in entomopathogenic nematodes of the genus Steinernema
46
Yaari, M., Department of Entomology and Nematology, Agricultural Research Organization, The Volcani Center, P.O. Box 6, Bet Dagan, Israel
Doron-Faigenboim, A., Department of Ornamental Horticulture, Agricultural Research Organization, Israel
Koltai, H., Department of Ornamental Horticulture, Agricultural Research Organization, Israel
Salame, L., Department of Entomology and Nematology, Agricultural Research Organization, The Volcani Center, P.O. Box 6, Bet Dagan, Israel
Glazer, I., Department of Entomology and Nematology, Agricultural Research Organization, The Volcani Center, P.O. Box 6, Bet Dagan, Israel
Transcriptome analysis of stress tolerance in entomopathogenic nematodes of the genus Steinernema
Entomopathogenic nematodes of the genus Steinernema are effective biological control agents. The infective stage of these parasites can withstand environmental stresses such as desiccation and heat, but the molecular and physiological mechanisms involved in this tolerance are poorly understood. We used 454 pyrosequencing to analyse transcriptome expression in Steinernema spp. that differ in their tolerance to stress. We compared these species, following heat and desiccation treatments, with their non-stressed counterparts. More than 98% of the transcripts found matched homologous sequences in the UniRef90 database, mostly nematode genes (85%). Among those, 60.8% aligned to the vertebrate parasites including Ascaris suum, Loa loa, and Brugia malayi, 23.3% aligned to bacteriovores, mostly from the genus Caenorhabditis, and 1% aligned to EPNs. Analysing gene expression patterns of the stress response showed a large fraction of down-regulated genes in the desiccation-tolerant nematode Steinernema riobrave, whereas a larger fraction of the genes in the susceptible Steinernema feltiae Carmiel and Gvulot strains were up-regulated. We further compared metabolic pathways and the expression of specific stress-related genes. In the more tolerant nematode, more genes were down-regulated whereas in the less tolerant strains, more genes were up-regulated. This phenomenon warrants further exploration of the mechanism governing induction of the down-regulation process. The present study revealed many genes and metabolic cycles that are differentially expressed in the stressed nematodes. Some of those are well known in other nematodes or anhydrobiotic organisms, but several are new and should be further investigated for their involvement in desiccation and heat tolerance. Our data establish a foundation for further exploration of stress tolerance in entomopathogenic nematodes and, in the long term, for improving their ability to withstand suboptimal environmental conditions. © 2015 Australian Society for Parasitology Inc..
Scientific Publication
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