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פותח על ידי קלירמאש פתרונות בע"מ -
Morphological control and mensuration of potato plantlets from tissue cultures for automated micropropagation
Year:
1994
Authors :
אלחנתי, ויקטור
;
.
Volume :
36
Co-Authors:
Alchanatis, V., Department of Agricultural Engineering, Technion-Israel Institute of Technology, Haifa, 32000, Israel
Peleg, K., Department of Agricultural Engineering, Technion-Israel Institute of Technology, Haifa, 32000, Israel
Ziv, M., Department of Agricultural Botany, The Hebrew University of Jerusalem, Rehovot, 76100, Israel
Facilitators :
From page:
331
To page:
338
(
Total pages:
8
)
Abstract:
Automation in plant micropropagation can be greatly simplified if the propagated plantlets have some morphological properties that facilitate automatic chopping and subsequent inspection and classification of the pre-cut plantlet segments by machine vision as viable propagules. We were able to control the morphogenic pattern of in vitro-propagated potato plantlets by adding various concentrations of ancymidol to the nutrient solution. It was found that plantlets cultured in 0.25 mg l-1 ancymidol best fit the requirements for automated mass micropropagation; the mean internode length was sufficiently large (9-10 mm), the color contrast between leaves and stems was significantly enhanced, the stem was thicker than in the control treatment and the number of axillary buds per plantlet was maximized. Microtuber formation on segments isolated from plants cultured in 0.25 and 0.5 mg l-1 ancymidol media was enhanced shortly after transfer to tuber induction medium in vitro. On shoot segments from control plants, microtuber formation started after 24-28 days. Machine vision was used to evaluate the morphological and color changes in cultured potato plants. Geometrical and color features such as the number of buds, internode length and color contrast between leaf and stem were precisely measured and automatically logged. Features were measured that till now could only be observed qualitatively. © 1994 Kluwer Academic Publishers.
Note:
Related Files :
ancymidol
color contrast
Machine vision
microtubers
plantlet morphology
עוד תגיות
תוכן קשור
More details
DOI :
10.1007/BF00046090
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
26812
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:25
Scientific Publication
Morphological control and mensuration of potato plantlets from tissue cultures for automated micropropagation
36
Alchanatis, V., Department of Agricultural Engineering, Technion-Israel Institute of Technology, Haifa, 32000, Israel
Peleg, K., Department of Agricultural Engineering, Technion-Israel Institute of Technology, Haifa, 32000, Israel
Ziv, M., Department of Agricultural Botany, The Hebrew University of Jerusalem, Rehovot, 76100, Israel
Morphological control and mensuration of potato plantlets from tissue cultures for automated micropropagation
Automation in plant micropropagation can be greatly simplified if the propagated plantlets have some morphological properties that facilitate automatic chopping and subsequent inspection and classification of the pre-cut plantlet segments by machine vision as viable propagules. We were able to control the morphogenic pattern of in vitro-propagated potato plantlets by adding various concentrations of ancymidol to the nutrient solution. It was found that plantlets cultured in 0.25 mg l-1 ancymidol best fit the requirements for automated mass micropropagation; the mean internode length was sufficiently large (9-10 mm), the color contrast between leaves and stems was significantly enhanced, the stem was thicker than in the control treatment and the number of axillary buds per plantlet was maximized. Microtuber formation on segments isolated from plants cultured in 0.25 and 0.5 mg l-1 ancymidol media was enhanced shortly after transfer to tuber induction medium in vitro. On shoot segments from control plants, microtuber formation started after 24-28 days. Machine vision was used to evaluate the morphological and color changes in cultured potato plants. Geometrical and color features such as the number of buds, internode length and color contrast between leaf and stem were precisely measured and automatically logged. Features were measured that till now could only be observed qualitatively. © 1994 Kluwer Academic Publishers.
Scientific Publication
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