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פותח על ידי קלירמאש פתרונות בע"מ -
Kinetic and temporal factors influence chilling injury to germinal vesicle and mature bovine oocytes
Year:
1999
Source of publication :
Cryobiology
Authors :
פרל, מיכל
;
.
Volume :
38
Co-Authors:
Zeron, Y., Institute of Animal Science, Agricultural Research Organization, Volcani Center, Bet Dagan, 50250, Israel
Pearl, M., Institute of Animal Science, Agricultural Research Organization, Volcani Center, Bet Dagan, 50250, Israel
Borochov, A., Kennedy-Leigh Ctr. for Hort. Res., Fac. Agric., Food, and Environ. Sci., Hebrew University of Jerusalem, Rehovot, 76100, Israel
Arav, A., Institute of Animal Science, Agricultural Research Organization, Volcani Center, Bet Dagan, 50250, Israel
Facilitators :
From page:
35
To page:
42
(
Total pages:
8
)
Abstract:
In this study we examined the effects of low, above freezing temperatures on the viability and functionality of bovine oocytes. Germinal vesicle (GV) stage and in vitro matured oocytes (MII) were exposed to various combinations of time (15 and 60 min) and temperature (4, 16, 23, and 39°C). After being treated, the ability of oocytes to undergo maturation and fertilization in vitro was examined, as well as their viability assayed by two fluorescent probes, fluorescein diacetate (FDA) and 5-carboxylfluorescein diacetate (cFDA). Cooling GV oocytes to 16°C for 15 min reduced the fertilization rate by more than 40%, compared with those left at 39°C. Surprisingly, cooling oocytes to 4°C reduced the fertilization rate by only 10% compared with control. Exposing GV oocytes to temperatures below 23°C reduced their viability. Similar to the reduction in fertilization, the viability of GV oocytes after exposure to 16°C was reduced by more than 50%, whereas exposure to 4°C reduced it by only 9%. Viability measurements using FDA and cFDA gave comparable results and showed a similar trend. The viability of MII oocytes and of GV oocytes pretreated with butylated hydroxytoluene, following exposure to low temperatures, was higher compared with that of GV controls. We interpret these results as indicating chilling effects on membrane integrity. Improving the chilling resistance of bovine oocytes may facilitate their short- and long-term preservation.
Note:
Related Files :
animal cell
Animals
cattle
chilling injury
Female
freezing
Germinal vesicle
induced hypothermia
עוד תגיות
תוכן קשור
More details
DOI :
10.1006/cryo.1998.2139
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
26842
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:25
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Scientific Publication
Kinetic and temporal factors influence chilling injury to germinal vesicle and mature bovine oocytes
38
Zeron, Y., Institute of Animal Science, Agricultural Research Organization, Volcani Center, Bet Dagan, 50250, Israel
Pearl, M., Institute of Animal Science, Agricultural Research Organization, Volcani Center, Bet Dagan, 50250, Israel
Borochov, A., Kennedy-Leigh Ctr. for Hort. Res., Fac. Agric., Food, and Environ. Sci., Hebrew University of Jerusalem, Rehovot, 76100, Israel
Arav, A., Institute of Animal Science, Agricultural Research Organization, Volcani Center, Bet Dagan, 50250, Israel
Kinetic and temporal factors influence chilling injury to germinal vesicle and mature bovine oocytes
In this study we examined the effects of low, above freezing temperatures on the viability and functionality of bovine oocytes. Germinal vesicle (GV) stage and in vitro matured oocytes (MII) were exposed to various combinations of time (15 and 60 min) and temperature (4, 16, 23, and 39°C). After being treated, the ability of oocytes to undergo maturation and fertilization in vitro was examined, as well as their viability assayed by two fluorescent probes, fluorescein diacetate (FDA) and 5-carboxylfluorescein diacetate (cFDA). Cooling GV oocytes to 16°C for 15 min reduced the fertilization rate by more than 40%, compared with those left at 39°C. Surprisingly, cooling oocytes to 4°C reduced the fertilization rate by only 10% compared with control. Exposing GV oocytes to temperatures below 23°C reduced their viability. Similar to the reduction in fertilization, the viability of GV oocytes after exposure to 16°C was reduced by more than 50%, whereas exposure to 4°C reduced it by only 9%. Viability measurements using FDA and cFDA gave comparable results and showed a similar trend. The viability of MII oocytes and of GV oocytes pretreated with butylated hydroxytoluene, following exposure to low temperatures, was higher compared with that of GV controls. We interpret these results as indicating chilling effects on membrane integrity. Improving the chilling resistance of bovine oocytes may facilitate their short- and long-term preservation.
Scientific Publication
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