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פותח על ידי קלירמאש פתרונות בע"מ -
Detection and Differentiation of Potato Virus Y Strains by Melting Analysis of an Oligonucleotide Virus Probe
Year:
2016
Authors :
רוזנר, אריה
;
.
Volume :
93
Co-Authors:
Rotem, N., The R.H. Smith Institute of Plant Sciences and Genetics in Agriculture, R.H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel
Shtein, C., The R.H. Smith Institute of Plant Sciences and Genetics in Agriculture, R.H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel
Rosner, A., The Department of Virology, Institute of Plant Protection, The Volcani Center, ARO, Bet Dagan, Israel
Levy, D., The R.H. Smith Institute of Plant Sciences and Genetics in Agriculture, R.H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel
Rabinowitch, H.D., The R.H. Smith Institute of Plant Sciences and Genetics in Agriculture, R.H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel
Facilitators :
From page:
620
To page:
625
(
Total pages:
6
)
Abstract:
A novel simple, fast qualitative method for detection and differentiation of PVY strains in potatoes by the LightCycler technology is described. Fluorescent-labeled probe designed to contain variable degree of homology with a ‘target sequence’ of several known reference PVY strains was annealed to PCR products of these viruses followed by a graded melting analysis. The specific characteristics of the melting curves enable the detection, distinction and differentiation of each of following four known PVY strains O-FL, O-RB, N and NTN, in a single reaction obviating the need for size or nucleotide sequence analyses. In addition, it was demonstrated that virus extracts from plants infected with more than one strain can be resolved using this procedure. The melting-curves of extracts from 55 market size tubers harvested randomly from commercial fields and compared with those of the reference virus strains, revealed the presence of O-FL, O-RB and NTN strains of PVY. © 2016, The Potato Association of America.
Note:
Related Files :
Genotyping
molecular analysis
PCR
PVY
RT-PCR
עוד תגיות
תוכן קשור
More details
DOI :
10.1007/s12230-016-9531-7
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
26866
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:26
Scientific Publication
Detection and Differentiation of Potato Virus Y Strains by Melting Analysis of an Oligonucleotide Virus Probe
93
Rotem, N., The R.H. Smith Institute of Plant Sciences and Genetics in Agriculture, R.H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel
Shtein, C., The R.H. Smith Institute of Plant Sciences and Genetics in Agriculture, R.H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel
Rosner, A., The Department of Virology, Institute of Plant Protection, The Volcani Center, ARO, Bet Dagan, Israel
Levy, D., The R.H. Smith Institute of Plant Sciences and Genetics in Agriculture, R.H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel
Rabinowitch, H.D., The R.H. Smith Institute of Plant Sciences and Genetics in Agriculture, R.H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel
Detection and Differentiation of Potato Virus Y Strains by Melting Analysis of an Oligonucleotide Virus Probe
A novel simple, fast qualitative method for detection and differentiation of PVY strains in potatoes by the LightCycler technology is described. Fluorescent-labeled probe designed to contain variable degree of homology with a ‘target sequence’ of several known reference PVY strains was annealed to PCR products of these viruses followed by a graded melting analysis. The specific characteristics of the melting curves enable the detection, distinction and differentiation of each of following four known PVY strains O-FL, O-RB, N and NTN, in a single reaction obviating the need for size or nucleotide sequence analyses. In addition, it was demonstrated that virus extracts from plants infected with more than one strain can be resolved using this procedure. The melting-curves of extracts from 55 market size tubers harvested randomly from commercial fields and compared with those of the reference virus strains, revealed the presence of O-FL, O-RB and NTN strains of PVY. © 2016, The Potato Association of America.
Scientific Publication
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