חיפוש מתקדם
תחביר
חפש...
הספר "אוצר וולקני"
אודות
תנאי שימוש
ניהול
קהילה:
אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
A microRNA of infectious laryngotracheitis virus can downregulate and direct cleavage of ICP4 mRNA
Year:
2011
Source of publication :
Virology
Authors :
גרמן, מרסלו
;
.
Volume :
411
Co-Authors:
Waidner, L.A., Elcriton, Inc. 15 Innovation Way, Suite 288, Newark, DE 19711, United States
Burnside, J., Department of Animal and Food Sciences, Delaware Biotechnology Institute, University of Delaware, Newark, DE 19711, United States
Anderson, A.S., Department of Animal and Food Sciences, Delaware Biotechnology Institute, University of Delaware, Newark, DE 19711, United States
Bernberg, E.L., Department of Animal and Food Sciences, Delaware Biotechnology Institute, University of Delaware, Newark, DE 19711, United States
German, M.A., Dow AgroSciences, LLC, 16160 SW Upper Boones Ferry Road, Portland, OR 97224, United States
Meyers, B.C., Department of Plant and Soil Sciences, Delaware Biotechnology Institute, University of Delaware, Newark, DE 19711, United States
Green, P.J., Department of Plant and Soil Sciences, Delaware Biotechnology Institute, University of Delaware, Newark, DE 19711, United States
Morgan, R.W., Department of Animal and Food Sciences, Delaware Biotechnology Institute, University of Delaware, Newark, DE 19711, United States
Facilitators :
From page:
25
To page:
31
(
Total pages:
7
)
Abstract:
Viral microRNAs regulate gene expression using either translational repression or mRNA cleavage and decay. Two microRNAs from infectious laryngotracheitis virus (ILTV), iltv-miR-I5 and iltv-miR-I6, map antisense to the ICP4 gene. Post-transcriptional repression by these microRNAs was tested against a portion of the ICP4 coding sequence cloned downstream of firefly luciferase. Luciferase activity was downregulated by approximately 60% with the iltv-miR-I5 mimic. Addition of an iltv-miR-I5 antagomiR or mutagenesis of the target seed sequence alleviated this effect. The iltv-miR-I5 mimic, when co-transfected with a plasmid expressing ICP4, reduced ICP4 transcript levels by approximately 50%, and inhibition was relieved by an iltv-miR-I5 antagomiR. In infected cells, iltv-miR-I5 mediated cleavage at the canonical site, as indicated by modified RACE analysis. Thus, in this system, iltv-miR-I5 decreased ILTV ICP4 mRNA levels via transcript cleavage and degradation. Downregulation of ICP4 could impact the balance between the lytic and latent states of the virus in vivo. © 2010 Elsevier Inc.
Note:
Related Files :
Animals
Artificial Gene Fusion
microRNA 15
Plasmid
RNA
sequence analysis
unclassified drug
virus RNA
עוד תגיות
תוכן קשור
More details
DOI :
10.1016/j.virol.2010.12.023
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
26902
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:26
You may also be interested in
Scientific Publication
A microRNA of infectious laryngotracheitis virus can downregulate and direct cleavage of ICP4 mRNA
411
Waidner, L.A., Elcriton, Inc. 15 Innovation Way, Suite 288, Newark, DE 19711, United States
Burnside, J., Department of Animal and Food Sciences, Delaware Biotechnology Institute, University of Delaware, Newark, DE 19711, United States
Anderson, A.S., Department of Animal and Food Sciences, Delaware Biotechnology Institute, University of Delaware, Newark, DE 19711, United States
Bernberg, E.L., Department of Animal and Food Sciences, Delaware Biotechnology Institute, University of Delaware, Newark, DE 19711, United States
German, M.A., Dow AgroSciences, LLC, 16160 SW Upper Boones Ferry Road, Portland, OR 97224, United States
Meyers, B.C., Department of Plant and Soil Sciences, Delaware Biotechnology Institute, University of Delaware, Newark, DE 19711, United States
Green, P.J., Department of Plant and Soil Sciences, Delaware Biotechnology Institute, University of Delaware, Newark, DE 19711, United States
Morgan, R.W., Department of Animal and Food Sciences, Delaware Biotechnology Institute, University of Delaware, Newark, DE 19711, United States
A microRNA of infectious laryngotracheitis virus can downregulate and direct cleavage of ICP4 mRNA
Viral microRNAs regulate gene expression using either translational repression or mRNA cleavage and decay. Two microRNAs from infectious laryngotracheitis virus (ILTV), iltv-miR-I5 and iltv-miR-I6, map antisense to the ICP4 gene. Post-transcriptional repression by these microRNAs was tested against a portion of the ICP4 coding sequence cloned downstream of firefly luciferase. Luciferase activity was downregulated by approximately 60% with the iltv-miR-I5 mimic. Addition of an iltv-miR-I5 antagomiR or mutagenesis of the target seed sequence alleviated this effect. The iltv-miR-I5 mimic, when co-transfected with a plasmid expressing ICP4, reduced ICP4 transcript levels by approximately 50%, and inhibition was relieved by an iltv-miR-I5 antagomiR. In infected cells, iltv-miR-I5 mediated cleavage at the canonical site, as indicated by modified RACE analysis. Thus, in this system, iltv-miR-I5 decreased ILTV ICP4 mRNA levels via transcript cleavage and degradation. Downregulation of ICP4 could impact the balance between the lytic and latent states of the virus in vivo. © 2010 Elsevier Inc.
Scientific Publication
נגישות
menu      
You may also be interested in