נגישות
menu      
חיפוש מתקדם
Phytoparasitica
Orenstein, J., Dept. of Plant Pathology, ARO, Gilat Experiment Station, Negev Post 2, 85410, Israel
Nachmias, A., Dept. of Plant Pathology, ARO, Gilat Experiment Station, Negev Post 2, 85410, Israel
Lips, H., Dept. of Biology, Ben-Gurion University, Be'er Sheva, Israel
To assess the relationship between Verticilliun dahliae toxin and the stunting effect in V. dahliae-infected plants, an assay was developed based on the inhibition of elongation of cultured tomato roots. The toxin inhibited growth of roots and development of secondary roots in disease-susceptible plants, tomato cv. 'Hosen-Eilon'(veve) and eggplant, whereas root growth was enhanced in tomato cv. 'VF-134', which carries the Ve gene for resistance, and in a non-host plant, onion. The toxin also inhibited the incorporation of [14C]-glutamic acid and [14C]-uridine into TCA-precipitable material in susceptible but not in resistant tomato roots. Cells from toxin-treated susceptible roots were smaller than those from resistant roots, suggesting that the toxin impeded cell growth rather than cell division. Since toxin-induced stunting cannot be detected using leaf bioassays, we suggest that the root inhibition assay can be used as a reliable tool for Verticillium disease tolerance screening studies with the V. dahliae toxin as probe. © 1989 Springer Science + Business Media B.V.
פותח על ידי קלירמאש פתרונות בע"מ -
הספר "אוצר וולקני"
אודות
תנאי שימוש
Inhibition of Tomato Root Growth in Culture by a Verticillium Dahliae Phytotoxin
17
Orenstein, J., Dept. of Plant Pathology, ARO, Gilat Experiment Station, Negev Post 2, 85410, Israel
Nachmias, A., Dept. of Plant Pathology, ARO, Gilat Experiment Station, Negev Post 2, 85410, Israel
Lips, H., Dept. of Biology, Ben-Gurion University, Be'er Sheva, Israel
Inhibition of Tomato Root Growth in Culture by a Verticillium Dahliae Phytotoxin
To assess the relationship between Verticilliun dahliae toxin and the stunting effect in V. dahliae-infected plants, an assay was developed based on the inhibition of elongation of cultured tomato roots. The toxin inhibited growth of roots and development of secondary roots in disease-susceptible plants, tomato cv. 'Hosen-Eilon'(veve) and eggplant, whereas root growth was enhanced in tomato cv. 'VF-134', which carries the Ve gene for resistance, and in a non-host plant, onion. The toxin also inhibited the incorporation of [14C]-glutamic acid and [14C]-uridine into TCA-precipitable material in susceptible but not in resistant tomato roots. Cells from toxin-treated susceptible roots were smaller than those from resistant roots, suggesting that the toxin impeded cell growth rather than cell division. Since toxin-induced stunting cannot be detected using leaf bioassays, we suggest that the root inhibition assay can be used as a reliable tool for Verticillium disease tolerance screening studies with the V. dahliae toxin as probe. © 1989 Springer Science + Business Media B.V.
Scientific Publication
You may also be interested in