Seroussi, E., Institute of Animal Science, Volcani Center, Bet-Dagan 50250, Israel
Ron, M., Institute of Animal Science, Volcani Center, Bet-Dagan 50250, Israel
Kedra, D., Information Systems Department, Burnham Institute, San Diego, CA 92037, United States

Sequencing of a bi-allelic PCR product, which contains an allele with a deletion/insertion mutation results in a superimposed tracefile following the site of this shift mutation. A trace file of this type hampers the use of current computer programs for base calling. ShiftDetector analyses a sequencing trace file in order to discover if it is a superimposed sequence of two molecules that differ in a shift mutation of 1 to 25 bases. The program calculates a probability score for the existence of such a shift and reconstructs the sequence of the original molecule.