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פותח על ידי קלירמאש פתרונות בע"מ -
A novel bacterial symbiont in the nematode Spirocerca lupi
Year:
2012
Source of publication :
BMC Microbiology
Authors :
גנאים, מוראד
;
.
Volume :
12
Co-Authors:
Gottlieb, Y., Koret School of Veterinary Medicine, Hebrew University of Jerusalem, P.O.B 12, Rehovot, 76100, Israel
Lavy, E., Koret School of Veterinary Medicine, Hebrew University of Jerusalem, P.O.B 12, Rehovot, 76100, Israel
Kaufman, M., Koret School of Veterinary Medicine, Hebrew University of Jerusalem, P.O.B 12, Rehovot, 76100, Israel
Markovics, A., Kimron Veterinary Institute, P.O.B 12, Bet-Dagan, 50250, Israel
Ghanim, M., Department of Entomology, Agricultural Research Organization, Volcani Center, P.O.B 6, Bet-Dagan, 50250, Israel
Aroch, I., Koret School of Veterinary Medicine, Hebrew University of Jerusalem, P.O.B 12, Rehovot, 76100, Israel
Facilitators :
From page:
To page:
(
Total pages:
1
)
Abstract:
Background: The parasitic nematode Spirocerca lupi (Spirurida: Thelaziidae), the canine esophageal worm, is the causative agent of spirocercosis, a disease causing morbidity and mortality in dogs. Spirocerca lupi has a complex life cycle, involving an obligatory coleopteran intermediate host (vector), an optional paratenic host, and a definitive canid host. The diagnosis of spirocercosis is challenging, especially in the early disease stages, when adult worms and clinical signs are absent. Thus, alternative approaches are needed to promote early diagnosis. The interaction between nematodes and their bacterial symbionts has recently become a focus of novel treatment regimens for other helminthic diseases. Results: Using 16S rDNA-based molecular methods, here we found a novel bacterial symbiont in S. lupi that is closely related to Comamonas species (Brukholderiales: Comamonadaceae) of the beta-proteobacteria. Its DNA was detected in eggs, larvae and adult stages of S. lupi. Using fluorescent in situ hybridization technique, we localized Comamonas sp. to the gut epithelial cells of the nematode larvae. Specific PCR enabled the detection of this symbiont's DNA in blood obtained from dogs diagnosed with spirocercosis. Conclusions: The discovery of a new Comamonas sp. in S. lupi increase the complexity of the interactions among the organisms involved in this system, and may open innovative approaches for diagnosis and control of spirocercosis in dogs. © 2012Gottlieb et al.; licensee BioMed Central Ltd.
Note:
Related Files :
Animals
Canidae
Dog Diseases
Dogs
Nematoda
Spirocerca lupi
Spirurida
Symbiosis
עוד תגיות
תוכן קשור
More details
DOI :
10.1186/1471-2180-12-133
Article number:
133
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
26976
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:27
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Scientific Publication
A novel bacterial symbiont in the nematode Spirocerca lupi
12
Gottlieb, Y., Koret School of Veterinary Medicine, Hebrew University of Jerusalem, P.O.B 12, Rehovot, 76100, Israel
Lavy, E., Koret School of Veterinary Medicine, Hebrew University of Jerusalem, P.O.B 12, Rehovot, 76100, Israel
Kaufman, M., Koret School of Veterinary Medicine, Hebrew University of Jerusalem, P.O.B 12, Rehovot, 76100, Israel
Markovics, A., Kimron Veterinary Institute, P.O.B 12, Bet-Dagan, 50250, Israel
Ghanim, M., Department of Entomology, Agricultural Research Organization, Volcani Center, P.O.B 6, Bet-Dagan, 50250, Israel
Aroch, I., Koret School of Veterinary Medicine, Hebrew University of Jerusalem, P.O.B 12, Rehovot, 76100, Israel
A novel bacterial symbiont in the nematode Spirocerca lupi
Background: The parasitic nematode Spirocerca lupi (Spirurida: Thelaziidae), the canine esophageal worm, is the causative agent of spirocercosis, a disease causing morbidity and mortality in dogs. Spirocerca lupi has a complex life cycle, involving an obligatory coleopteran intermediate host (vector), an optional paratenic host, and a definitive canid host. The diagnosis of spirocercosis is challenging, especially in the early disease stages, when adult worms and clinical signs are absent. Thus, alternative approaches are needed to promote early diagnosis. The interaction between nematodes and their bacterial symbionts has recently become a focus of novel treatment regimens for other helminthic diseases. Results: Using 16S rDNA-based molecular methods, here we found a novel bacterial symbiont in S. lupi that is closely related to Comamonas species (Brukholderiales: Comamonadaceae) of the beta-proteobacteria. Its DNA was detected in eggs, larvae and adult stages of S. lupi. Using fluorescent in situ hybridization technique, we localized Comamonas sp. to the gut epithelial cells of the nematode larvae. Specific PCR enabled the detection of this symbiont's DNA in blood obtained from dogs diagnosed with spirocercosis. Conclusions: The discovery of a new Comamonas sp. in S. lupi increase the complexity of the interactions among the organisms involved in this system, and may open innovative approaches for diagnosis and control of spirocercosis in dogs. © 2012Gottlieb et al.; licensee BioMed Central Ltd.
Scientific Publication
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