נגישות
menu      
חיפוש מתקדם
תחביר
חפש...
הספר "אוצר וולקני"
אודות
תנאי שימוש
ניהול
קהילה:
אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
Isolation of viable deletion mutants of Streptomyces actinophage (Pal 6) and their molecular characterization
Year:
1980
Source of publication :
MGG Molecular & General Genetics
Authors :
רוזנר, אריה
;
.
Volume :
178
Co-Authors:
Rosner, A., Department of Virology, Weizmann Institute of Science, Rehovot, Israel
Gutstein, R., Department of Virology, Weizmann Institute of Science, Rehovot, Israel
Aviv, H., Department of Virology, Weizmann Institute of Science, Rehovot, Israel
Facilitators :
From page:
337
To page:
341
(
Total pages:
5
)
Abstract:
Deletion mutants of bacteriophage Pal 6 were isolated by successive treatments of either heat (60° C) or pyrophosphate (10 mM). These mutants were characterized by restriction enzyme cleavage analysis. The pyrophosphate resistant clones lost the whole Eco R1 fragment in which the Sal I site is located, as well as an unrelated Hind III fragment. These results show that the region containing the Sal I site in the phage genome is not essential for phage viability. This single Sal I site is therefore suitable as a potential insertion site for DNA cloning. On the other hand, the heat resistant clones that were isolated and characterized do not appear to have detectable deletions as indicated by their Eco R1 DNA digestion pattern. © 1980 Springer-Verlag.
Note:
Related Files :
animal experiment
Centrifugation, Density Gradient
Diphosphates
Heredity
mutation
phenotype
Streptomycetaceae
עוד תגיות
תוכן קשור
More details
DOI :
10.1007/BF00270481
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
26981
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:27
Scientific Publication
Isolation of viable deletion mutants of Streptomyces actinophage (Pal 6) and their molecular characterization
178
Rosner, A., Department of Virology, Weizmann Institute of Science, Rehovot, Israel
Gutstein, R., Department of Virology, Weizmann Institute of Science, Rehovot, Israel
Aviv, H., Department of Virology, Weizmann Institute of Science, Rehovot, Israel
Isolation of viable deletion mutants of Streptomyces actinophage (Pal 6) and their molecular characterization
Deletion mutants of bacteriophage Pal 6 were isolated by successive treatments of either heat (60° C) or pyrophosphate (10 mM). These mutants were characterized by restriction enzyme cleavage analysis. The pyrophosphate resistant clones lost the whole Eco R1 fragment in which the Sal I site is located, as well as an unrelated Hind III fragment. These results show that the region containing the Sal I site in the phage genome is not essential for phage viability. This single Sal I site is therefore suitable as a potential insertion site for DNA cloning. On the other hand, the heat resistant clones that were isolated and characterized do not appear to have detectable deletions as indicated by their Eco R1 DNA digestion pattern. © 1980 Springer-Verlag.
Scientific Publication
You may also be interested in