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Ovarian cysts in high-yielding dairy cows
Year:
2009
Source of publication :
Theriogenology
Authors :
בראב-טל, רות
;
.
פן, סווטלנה
;
.
Volume :
72
Co-Authors:
Braw-Tal, R., Agricultural Research Organization, Institute of Animal Science, Bet Dagan, 50250, Israel
Pen, S., Agricultural Research Organization, Institute of Animal Science, Bet Dagan, 50250, Israel
Roth, Z., Department of Animal Science, Robert H. Smith Faculty of Agriculture, Food and Environment, Rehovot, 76100, Israel
Facilitators :
From page:
690
To page:
698
(
Total pages:
9
)
Abstract:
We examined the hormonal and morphologic changes associated with ovarian cyst formation in high-yielding dairy cows. Follicle fluid was aspirated from 90 cysts and 15 preovulatory and 18 subordinate follicles and used for hormonal determination. Pieces of cystic wall were subjected to morphologic and immunohistochemical evaluation. Cysts were characterized by low concentrations of insulin, insulin-like growth factor-I (IGF-I), and glucose and high activity of IGF binding proteins (IGFBPs). Insulin and IGF-I levels were (mean ± SEM) 205 ± 22 pg/mL and 146 ± 42 ng/mL in preovulatory follicles and 3 ± 1 pg/mL and 61 ± 6 ng/mL in cysts, respectively (P < 0.001). Insulin-like growth factor-binding proteins activity was about 10 times higher in cysts than in preovulatory follicles. Cysts were classified into three types according to their estradiol-to-progesterone (E/P) ratio. Type 1 cysts (n = 23) exhibited the highest E/P ratio (10.8 ± 2.3), partial loss of granulosa cells, and severe morphologic changes in the theca interna. Expression of P450 side-chain cleavage and P450 17α-hydroxylase was noted in theca cells and expression of inhibin-α in granulosa cells. Type 2 cysts (n = 35) had a low E/P ratio (0.07 ± 0.02), and patches of luteal-like tissue in the cystic wall. Type 3 cysts (n = 32) had an E/P ratio of 0.91 ± 0.17, and no recognizable granulosa or theca cells. In summary, intrafollicular steroid levels as expressed by E/P ratio, together with IGF-I and insulin levels and morphologic changes in the follicular wall, may serve as accurate cyst-classification parameters. Because IGF-I and/or insulin play an essential role in the final stage of follicle development, it can be speculated that abnormal levels of these metabolic hormones might lead to follicle dysfunction, resulting in follicular regression or cyst formation. © 2009 Elsevier Inc. All rights reserved.
Note:
Related Files :
Animal
Animals
cattle
chemistry
Female
Follicular Fluid
Insulin-Like Growth Factor I
metabolism
Pathology
עוד תגיות
תוכן קשור
More details
DOI :
10.1016/j.theriogenology.2009.04.027
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
26999
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:27
You may also be interested in
Scientific Publication
Ovarian cysts in high-yielding dairy cows
72
Braw-Tal, R., Agricultural Research Organization, Institute of Animal Science, Bet Dagan, 50250, Israel
Pen, S., Agricultural Research Organization, Institute of Animal Science, Bet Dagan, 50250, Israel
Roth, Z., Department of Animal Science, Robert H. Smith Faculty of Agriculture, Food and Environment, Rehovot, 76100, Israel
Ovarian cysts in high-yielding dairy cows
We examined the hormonal and morphologic changes associated with ovarian cyst formation in high-yielding dairy cows. Follicle fluid was aspirated from 90 cysts and 15 preovulatory and 18 subordinate follicles and used for hormonal determination. Pieces of cystic wall were subjected to morphologic and immunohistochemical evaluation. Cysts were characterized by low concentrations of insulin, insulin-like growth factor-I (IGF-I), and glucose and high activity of IGF binding proteins (IGFBPs). Insulin and IGF-I levels were (mean ± SEM) 205 ± 22 pg/mL and 146 ± 42 ng/mL in preovulatory follicles and 3 ± 1 pg/mL and 61 ± 6 ng/mL in cysts, respectively (P < 0.001). Insulin-like growth factor-binding proteins activity was about 10 times higher in cysts than in preovulatory follicles. Cysts were classified into three types according to their estradiol-to-progesterone (E/P) ratio. Type 1 cysts (n = 23) exhibited the highest E/P ratio (10.8 ± 2.3), partial loss of granulosa cells, and severe morphologic changes in the theca interna. Expression of P450 side-chain cleavage and P450 17α-hydroxylase was noted in theca cells and expression of inhibin-α in granulosa cells. Type 2 cysts (n = 35) had a low E/P ratio (0.07 ± 0.02), and patches of luteal-like tissue in the cystic wall. Type 3 cysts (n = 32) had an E/P ratio of 0.91 ± 0.17, and no recognizable granulosa or theca cells. In summary, intrafollicular steroid levels as expressed by E/P ratio, together with IGF-I and insulin levels and morphologic changes in the follicular wall, may serve as accurate cyst-classification parameters. Because IGF-I and/or insulin play an essential role in the final stage of follicle development, it can be speculated that abnormal levels of these metabolic hormones might lead to follicle dysfunction, resulting in follicular regression or cyst formation. © 2009 Elsevier Inc. All rights reserved.
Scientific Publication
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