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פותח על ידי קלירמאש פתרונות בע"מ -
Genetic transformation of citrus protoplasts and regeneration of transgenic plants
Year:
1990
Source of publication :
Plant Science
Authors :
ורדי, עליזה
;
.
Volume :
69
Co-Authors:
Vardi, A., Institute of Horticulture, Agricultural Research Organization, The Volcani Center, Bet Dagan, 50250, Israel
Bleichman, S., Department of Plant Genetics, The Weizmann Institute of Science, Rehovot, 76100, Israel
Aviv, D., Department of Plant Genetics, The Weizmann Institute of Science, Rehovot, 76100, Israel
Facilitators :
From page:
199
To page:
206
(
Total pages:
8
)
Abstract:
Citrus protoplast-derived embryogenic calli can readily be induced to differentiate embryos which further develop into mature fruit bearing trees. We successfully introduced plasmid pCAP212 DNA, harboring the coding sequences of neomycin phosphotransferase (nptII) and chloramphenicol acetyltransferase (cat) genes into Citrus protoplasts via polyethylene glycol (PEG) treatment resulting in transgenic plants. Transient expression of chloramphenicol acetyltransferase (CAT) activity was detected 3 days after the direct transformation was performed. Microcolonies (0.5 mm in diameter) were selected on agar medium containing paromomycin sulfate (PAR). Twenty one resistant clones, presumably containing the nptII gene, were isolated. To verify the transgenic nature of the isolated clones either neomycin phosphotransferase (nptII) activity was measured or Southern hybridization was performed. Altogether 9 positive stably transformed embryogenic clones were obtained; two of these were regenerated into transgenic rooted plants. © 1990.
Note:
Related Files :
chloramphenicol acetyltransferase
Citrus
direct transformation
neomycin phosphotransferase
paromomycin
Protoplasts
transgenic plants
עוד תגיות
תוכן קשור
More details
DOI :
10.1016/0168-9452(90)90118-8
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
27005
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:27
You may also be interested in
Scientific Publication
Genetic transformation of citrus protoplasts and regeneration of transgenic plants
69
Vardi, A., Institute of Horticulture, Agricultural Research Organization, The Volcani Center, Bet Dagan, 50250, Israel
Bleichman, S., Department of Plant Genetics, The Weizmann Institute of Science, Rehovot, 76100, Israel
Aviv, D., Department of Plant Genetics, The Weizmann Institute of Science, Rehovot, 76100, Israel
Genetic transformation of citrus protoplasts and regeneration of transgenic plants
Citrus protoplast-derived embryogenic calli can readily be induced to differentiate embryos which further develop into mature fruit bearing trees. We successfully introduced plasmid pCAP212 DNA, harboring the coding sequences of neomycin phosphotransferase (nptII) and chloramphenicol acetyltransferase (cat) genes into Citrus protoplasts via polyethylene glycol (PEG) treatment resulting in transgenic plants. Transient expression of chloramphenicol acetyltransferase (CAT) activity was detected 3 days after the direct transformation was performed. Microcolonies (0.5 mm in diameter) were selected on agar medium containing paromomycin sulfate (PAR). Twenty one resistant clones, presumably containing the nptII gene, were isolated. To verify the transgenic nature of the isolated clones either neomycin phosphotransferase (nptII) activity was measured or Southern hybridization was performed. Altogether 9 positive stably transformed embryogenic clones were obtained; two of these were regenerated into transgenic rooted plants. © 1990.
Scientific Publication
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