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קהילה:
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פותח על ידי קלירמאש פתרונות בע"מ -
Induction of Cuticular Melanization in Spodoptera littoralis Larvae by PBAN/MRCH: Development of a Quantitative Bioassay and Structure Function Analysis
Year:
1996
Authors :
אלטשטיין, מרים
;
.
בן-עזיז, אורנה
;
.
גבאי, טל
;
.
גזית, יואב
;
.
מרכוס, רות
;
.
Volume :
31
Co-Authors:
Altstein, M., Department of Entomology, ARO, Volcani Center, Bet Dagan, Israel, Dept. of Entomology, ARO, Volcani Center, Bet Dagan 50250, Israel
Gazit, Y., Department of Entomology, ARO, Volcani Center, Bet Dagan, Israel
Aziz, O.B., Department of Entomology, ARO, Volcani Center, Bet Dagan, Israel
Gabay, T., Department of Entomology, ARO, Volcani Center, Bet Dagan, Israel
Marcus, R., Department of Statistics, ARO, Volcani Center, Bet Dagan, Israel
Vogel, Z., Department of Neurobiology, Weizmann Institute of Science, Rehovot, Israel
Barg, J., Cardiovasc. Hypertension Res. Lab., Wolfson Medical Center, Tel Aviv University Medical School, Holon, Israel
Facilitators :
From page:
355
To page:
370
(
Total pages:
16
)
Abstract:
PBAN (also termed melanization and reddish coloration hormone, MRCH) is a cerebral factor known to regulate sex pheromone biosynthesis and cuticular melanization in moths. In the present study we developed a quantitative method (based on computerized image analysis of cuticles) to determine the effect of Helicoverpa zea PBAN (Hez-PBAN) on cuticular melanization and to study the structure-activity relationship of the neuropeptide in Spodoptera littoralis larvae. The results indicate that Hez-PBAN stimulates cuticular melanization in an interspecific manner, and that the minimal dose evoking formation of melanins is between 3-10 pmol/larva. Higher doses of Hez-PBAN did not stimulate melanization any further. Examination of the structure-activity relationship of Hez-PBAN revealed that the first eight N-terminal amino acids are not essential for the melanotropic activity and that the activity resides in the C-terminal region. Within this region the C-terminal amide was found to play a very important role. © 1996 Wiley-Liss, Inc.
Note:
Related Files :
Insect neuropeptide
Melanization
Moths
MRCH
PBAN
Spodoptera littoralis
עוד תגיות
תוכן קשור
More details
DOI :
Article number:
0
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
27029
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:27
You may also be interested in
Scientific Publication
Induction of Cuticular Melanization in Spodoptera littoralis Larvae by PBAN/MRCH: Development of a Quantitative Bioassay and Structure Function Analysis
31
Altstein, M., Department of Entomology, ARO, Volcani Center, Bet Dagan, Israel, Dept. of Entomology, ARO, Volcani Center, Bet Dagan 50250, Israel
Gazit, Y., Department of Entomology, ARO, Volcani Center, Bet Dagan, Israel
Aziz, O.B., Department of Entomology, ARO, Volcani Center, Bet Dagan, Israel
Gabay, T., Department of Entomology, ARO, Volcani Center, Bet Dagan, Israel
Marcus, R., Department of Statistics, ARO, Volcani Center, Bet Dagan, Israel
Vogel, Z., Department of Neurobiology, Weizmann Institute of Science, Rehovot, Israel
Barg, J., Cardiovasc. Hypertension Res. Lab., Wolfson Medical Center, Tel Aviv University Medical School, Holon, Israel
Induction of Cuticular Melanization in Spodoptera littoralis Larvae by PBAN/MRCH: Development of a Quantitative Bioassay and Structure Function Analysis
PBAN (also termed melanization and reddish coloration hormone, MRCH) is a cerebral factor known to regulate sex pheromone biosynthesis and cuticular melanization in moths. In the present study we developed a quantitative method (based on computerized image analysis of cuticles) to determine the effect of Helicoverpa zea PBAN (Hez-PBAN) on cuticular melanization and to study the structure-activity relationship of the neuropeptide in Spodoptera littoralis larvae. The results indicate that Hez-PBAN stimulates cuticular melanization in an interspecific manner, and that the minimal dose evoking formation of melanins is between 3-10 pmol/larva. Higher doses of Hez-PBAN did not stimulate melanization any further. Examination of the structure-activity relationship of Hez-PBAN revealed that the first eight N-terminal amino acids are not essential for the melanotropic activity and that the activity resides in the C-terminal region. Within this region the C-terminal amide was found to play a very important role. © 1996 Wiley-Liss, Inc.
Scientific Publication
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