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קהילה:
אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
Identification and localization of a Rickettsia sp. in Bemisia tabaci (Homoptera: Aleyrodidae)
Year:
2006
Authors :
בלאוסוב, אדוארד
;
.
גוטליב, יובל
;
.
גנאים, מוראד
;
.
הורוביץ, רמי
;
.
מוזס-דאובה, נטע
;
.
פורטנוי, ויטלי
;
.
צורי, גליל
;
.
צחורי-פיין, עינת
;
.
קונצדלוב, סבטלנה
;
.
קציר, נורית
;
.
Volume :
72
Co-Authors:


 
Chiel, E., Bio-Bee Biological Systems, Kibbutz Sde Eliyahu, Beit Shean Valley 10810, Israel
Gerling, D., Tel Aviv University, Department of Zoology, Ramat Aviv 69978, Israel
Steinberg, S., Bio-Bee Biological Systems, Kibbutz Sde Eliyahu, Beit Shean Valley 10810, Israel
Gershon, M., Tel Aviv University, Department of Zoology, Ramat Aviv 69978, Israel
Gal, S., Tel Aviv University, Department of Zoology, Ramat Aviv 69978, Israel

 

Facilitators :
From page:
3646
To page:
3652
(
Total pages:
7
)
Abstract:
Whiteflies (Homoptera: Aleyrodidae) are sap-sucking insects that harbor "Candidatus Portiera aleyrodidarum," an obligatory symbiotic bacterium which is housed in a special organ called the bacteriome. These insects are also home for a diverse facultative microbial community which may include Hamiltonella, Arsenophonus, Fritchea, Wolbachia, and Cardinium spp. In this study, the bacteria associated with a B biotype of the sweet potato whitefly Bemisia tabaci were characterized using molecular fingerprinting techniques, and a Rickettsia sp. was detected for the first time in this insect family. Rickettsia sp. distribution, transmission and localization were studied using PCR and fluorescence in situ hybridizations (FISH). Rickettsia was found in all 20 Israeli B. tabaci populations screened but not in all individuals within each population. A FISH analysis of B. tabaci eggs, nymphs, and adults revealed a unique concentration of Rickettsia around the gut and follicle cells, as well as a random distribution in the hemolymph. We postulate that the Rickettsia enters the oocyte together with the bacteriocytes, leaves these symbiont-housing cells when the egg is laid, multiplies and spreads throughout the egg during embryogenesis and, subsequently, disperses throughout the body of the hatching nymph, excluding the bacteriomes. Although the role Rickettsia plays in the biology of the whitefly is currently unknown, the vertical transmission on the one hand and the partial within-population infection on the other suggest a phenotype that is advantageous under certain conditions but may be deleterious enough to prevent fixation under others. Copyright © 2006, American Society for Microbiology. All Rights Reserved.
Note:
Related Files :
Animals
bacteria
Bemisia tabaci
Female
fish
hybridization
Israel
phenotype
Symbiosis
עוד תגיות
תוכן קשור
More details
DOI :
10.1128/AEM.72.5.3646-3652.2006
Article number:
0
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
27032
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:27
Scientific Publication
Identification and localization of a Rickettsia sp. in Bemisia tabaci (Homoptera: Aleyrodidae)
72


 
Chiel, E., Bio-Bee Biological Systems, Kibbutz Sde Eliyahu, Beit Shean Valley 10810, Israel
Gerling, D., Tel Aviv University, Department of Zoology, Ramat Aviv 69978, Israel
Steinberg, S., Bio-Bee Biological Systems, Kibbutz Sde Eliyahu, Beit Shean Valley 10810, Israel
Gershon, M., Tel Aviv University, Department of Zoology, Ramat Aviv 69978, Israel
Gal, S., Tel Aviv University, Department of Zoology, Ramat Aviv 69978, Israel

 

Identification and localization of a Rickettsia sp. in Bemisia tabaci (Homoptera: Aleyrodidae)
Whiteflies (Homoptera: Aleyrodidae) are sap-sucking insects that harbor "Candidatus Portiera aleyrodidarum," an obligatory symbiotic bacterium which is housed in a special organ called the bacteriome. These insects are also home for a diverse facultative microbial community which may include Hamiltonella, Arsenophonus, Fritchea, Wolbachia, and Cardinium spp. In this study, the bacteria associated with a B biotype of the sweet potato whitefly Bemisia tabaci were characterized using molecular fingerprinting techniques, and a Rickettsia sp. was detected for the first time in this insect family. Rickettsia sp. distribution, transmission and localization were studied using PCR and fluorescence in situ hybridizations (FISH). Rickettsia was found in all 20 Israeli B. tabaci populations screened but not in all individuals within each population. A FISH analysis of B. tabaci eggs, nymphs, and adults revealed a unique concentration of Rickettsia around the gut and follicle cells, as well as a random distribution in the hemolymph. We postulate that the Rickettsia enters the oocyte together with the bacteriocytes, leaves these symbiont-housing cells when the egg is laid, multiplies and spreads throughout the egg during embryogenesis and, subsequently, disperses throughout the body of the hatching nymph, excluding the bacteriomes. Although the role Rickettsia plays in the biology of the whitefly is currently unknown, the vertical transmission on the one hand and the partial within-population infection on the other suggest a phenotype that is advantageous under certain conditions but may be deleterious enough to prevent fixation under others. Copyright © 2006, American Society for Microbiology. All Rights Reserved.
Scientific Publication
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