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פותח על ידי קלירמאש פתרונות בע"מ -
Identification of a tau promoter region mediating tissue-specific-regulated expression in PC12 cells
Year:
1996
Source of publication :
Journal of Molecular Biology
Authors :
שדות, עינת
;
.
Volume :
256
Co-Authors:
Sadot, E., Department of Neurobiology, Weizmann Institute of Science, 76100 Rehovot, Israel
Heicklen-Klein, A., Department of Neurobiology, Weizmann Institute of Science, 76100 Rehovot, Israel
Barg, J., Department of Neurobiology, Weizmann Institute of Science, 76100 Rehovot, Israel, Therapeutic Community Ramot Yehuda, Zoharim, Israel
Lazarovici, P., Department of Pharmacology, School of Pharmacy, Hebrew University of Jerusalem, 91120 Jerusalem, Israel
Ginzburg, I., Department of Neurobiology, Weizmann Institute of Science, 76100 Rehovot, Israel
Facilitators :
From page:
805
To page:
812
(
Total pages:
8
)
Abstract:
Tau, a microtubule-associated protein, is encoded by a single gene, the expression of which is neuron-specific and developmentally regulated. When PC12 cells are exposed to nerve growth factor (NGF), they differentiate to sympathetic-like neurons. This differentiation process is accompanied by an elevation of tau proteins and mRNA. Here, we describe, for the first time, the isolation and characterization of a tau promoter region. We show that the promoter of tau is G + C-rich, lacks a genuine TATA box and thus promotes multiple initiation sites of RNA transcription. Our results demonstrate that a region of approximately 335 base-pairs residing immediately upstream of tau exon -1 are able to direct positive control of neuron-specific activity of the luciferase reporter gene. The isolation of tau promoter will facilitate further studies of the regulation of tau expression during development and aging of neuronal cells.
Note:
Related Files :
animal cell
Animals
Base Sequence
cell strain
Neuronal promoter
PC12 Cells
Tau
עוד תגיות
תוכן קשור
More details
DOI :
10.1006/jmbi.1996.0126
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
27034
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:27
You may also be interested in
Scientific Publication
Identification of a tau promoter region mediating tissue-specific-regulated expression in PC12 cells
256
Sadot, E., Department of Neurobiology, Weizmann Institute of Science, 76100 Rehovot, Israel
Heicklen-Klein, A., Department of Neurobiology, Weizmann Institute of Science, 76100 Rehovot, Israel
Barg, J., Department of Neurobiology, Weizmann Institute of Science, 76100 Rehovot, Israel, Therapeutic Community Ramot Yehuda, Zoharim, Israel
Lazarovici, P., Department of Pharmacology, School of Pharmacy, Hebrew University of Jerusalem, 91120 Jerusalem, Israel
Ginzburg, I., Department of Neurobiology, Weizmann Institute of Science, 76100 Rehovot, Israel
Identification of a tau promoter region mediating tissue-specific-regulated expression in PC12 cells
Tau, a microtubule-associated protein, is encoded by a single gene, the expression of which is neuron-specific and developmentally regulated. When PC12 cells are exposed to nerve growth factor (NGF), they differentiate to sympathetic-like neurons. This differentiation process is accompanied by an elevation of tau proteins and mRNA. Here, we describe, for the first time, the isolation and characterization of a tau promoter region. We show that the promoter of tau is G + C-rich, lacks a genuine TATA box and thus promotes multiple initiation sites of RNA transcription. Our results demonstrate that a region of approximately 335 base-pairs residing immediately upstream of tau exon -1 are able to direct positive control of neuron-specific activity of the luciferase reporter gene. The isolation of tau promoter will facilitate further studies of the regulation of tau expression during development and aging of neuronal cells.
Scientific Publication
You may also be interested in