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פותח על ידי קלירמאש פתרונות בע"מ -
Responses of micropropagated peach cultivars to thermotherapy for the elimination of prunus necrotic ringspot virus
Year:
1991
Source of publication :
Annals of Applied Biology
Authors :
לוי, שמואל
;
.
פיינגרש (וייסמן), יבגניה
;
.
ריגר-שטיין, עדינה
;
.
שפיגל, שרה
;
.
Volume :
119
Co-Authors:
STEIN, A., Agricultural Research Organization, Volcani Center, Bet Dagan, 50250, Israel
SPIEGEL, S., Agricultural Research Organization, Volcani Center, Bet Dagan, 50250, Israel
FAINGERSH, G., Agricultural Research Organization, Volcani Center, Bet Dagan, 50250, Israel
LEVY, S., Agricultural Research Organization, Volcani Center, Bet Dagan, 50250, Israel
Facilitators :
From page:
265
To page:
271
(
Total pages:
7
)
Abstract:
Peach (Prunus persica (L.) Batsch) shoot‐cultures infected with prunus necrotic ringspot virus (PNRSV) were selected for evaluating the responses of in vitro grown shoots of cvs Hermosa and Summerset to thermotherapy. The survival of shoot‐cultures during thermotherapy was improved by selection of the optimum concentration of 6‐benzylamino purine in the medium and optimum age of shoots for treatment. Alternating high and low temperature thermotherapy regimes were more effective in decreasing virus titre than constant high temperatures. Of the regimes tested, the most effective inhibition of PNRSV combined with a high survival of shoots was obtained by applying 38°C for 16 h in light alternating with 28 CC for 8 h in darkness for 18 days for Hermosa and 22 days for Summerset. Following this treatment 90% of Hermosa and 40% of Summerset shoot‐cultures were virus‐free as determined by enzyme‐linked immunosorbent assay. Relatively large (about 10 mm) apices excised from these shots regenerated into virus‐free plants. The advantage of the in vitro system for thermotherapy is discussed. Copyright © 1991, Wiley Blackwell. All rights reserved
Note:
Related Files :
In vitro
peach
Prunus necrotic ringspot virus
Thermotherapy
עוד תגיות
תוכן קשור
More details
DOI :
10.1111/j.1744-7348.1991.tb04865.x
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
27058
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:27
Scientific Publication
Responses of micropropagated peach cultivars to thermotherapy for the elimination of prunus necrotic ringspot virus
119
STEIN, A., Agricultural Research Organization, Volcani Center, Bet Dagan, 50250, Israel
SPIEGEL, S., Agricultural Research Organization, Volcani Center, Bet Dagan, 50250, Israel
FAINGERSH, G., Agricultural Research Organization, Volcani Center, Bet Dagan, 50250, Israel
LEVY, S., Agricultural Research Organization, Volcani Center, Bet Dagan, 50250, Israel
Responses of micropropagated peach cultivars to thermotherapy for the elimination of prunus necrotic ringspot virus
Peach (Prunus persica (L.) Batsch) shoot‐cultures infected with prunus necrotic ringspot virus (PNRSV) were selected for evaluating the responses of in vitro grown shoots of cvs Hermosa and Summerset to thermotherapy. The survival of shoot‐cultures during thermotherapy was improved by selection of the optimum concentration of 6‐benzylamino purine in the medium and optimum age of shoots for treatment. Alternating high and low temperature thermotherapy regimes were more effective in decreasing virus titre than constant high temperatures. Of the regimes tested, the most effective inhibition of PNRSV combined with a high survival of shoots was obtained by applying 38°C for 16 h in light alternating with 28 CC for 8 h in darkness for 18 days for Hermosa and 22 days for Summerset. Following this treatment 90% of Hermosa and 40% of Summerset shoot‐cultures were virus‐free as determined by enzyme‐linked immunosorbent assay. Relatively large (about 10 mm) apices excised from these shots regenerated into virus‐free plants. The advantage of the in vitro system for thermotherapy is discussed. Copyright © 1991, Wiley Blackwell. All rights reserved
Scientific Publication
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