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קהילה:
אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
Sequential expression of bacterial virulence and plant defense genes during infection of tomato with clavibacter michiganensis subsp. michiganensis
Year:
2010
Source of publication :
Phytopathology
Authors :
דרור, אורית
;
.
מנוליס-ששון, שולמית
;
.
צ'לופוביץ, לאורה
;
.
Volume :
100
Co-Authors:
Chalupowicz, L., Department of Plant Pathology and Weed Research, ARO, the Volcani Center, Bet Dagan, Israel, Department of Plant Sciences, Tel Aviv University, Israel
Cohen-Kandli, M., Department of Plant Pathology and Weed Research, ARO, the Volcani Center, Bet Dagan, Israel, Department of Plant Sciences, Tel Aviv University, Israel
Dror, O., Department of Plant Pathology and Weed Research, ARO, the Volcani Center, Bet Dagan, Israel
Eichenlaub R., Fakultät für Biologie, Gentechnologie/Mikrobiologie, Universität Bielefeld, Germany
Gartemann, K.-H., Fakultät für Biologie, Gentechnologie/Mikrobiologie, Universität Bielefeld, Germany
Sessa, G., Department of Plant Sciences, Tel Aviv University, Israel
Barash, I., Department of Plant Sciences, Tel Aviv University, Israel
Manulis-Sasson, S., Department of Plant Pathology and Weed Research, ARO, the Volcani Center, Bet Dagan, Israel
Facilitators :
From page:
252
To page:
261
(
Total pages:
10
)
Abstract:
Chalupowicz, L., Cohen-Kandli, M., Dror, O., Eichenlaub, R., Gartemann, K.-H., Sessa, G., Barash, I., and Manulis-Sasson, S. 2010. Sequential expression of bacterial virulence and plant defense genes during infection of tomato with Clavibacter michiganensis subsp. michiganensis. Phytopathology 100:252-261. The molecular interactions between Clavibacter michiganensis subsp. michiganensis and tomato plant were studied by following the expression of bacterial virulence and host-defense genes during early stages of infection. The C. michiganensis subsp. michiganensis genes included the plasmid-borne cellulase (celA) and the serine protease (pat-1), and the serine proteases chpC and ppaA, residing on the chp/tomA pathogenicity island (PAI). Gene expression was measured following tomato inoculation with Cmm382 (wild type), Cmm100 (lacking the plasmids pCM1 and pCM2), and Cmm27 (lacking the PAI). Transcriptional analysis revealed that celA and pat-1 were significantly induced in Cmm382 at initial 12 to 72 h, whereas chpC and ppaA were highly expressed only 96 h after inoculation. Interdependence between the expression of chromosomal and of plasmid-located genes was revealed: expression of celA and pat-1 was substantially reduced in the absence of the chp/tomA PAI, whereas chpC and ppaA expressions were reduced in the absence of the virulence plasmids. Transcription of chromosomal genes involved in cell wall degradation (i.e., pelA1, celB, xysA, and xysB), was also induced at early stages of infection. Expression of the host-defense genes, chitinase class II and pathogenesis-related protein-5 isoform was induced in the absence of the PAI at early stages of infection, suggesting that PAIlocated genes are involved in suppression of tomato basal defenses. © 2010 The American Phytopathological Society.
Note:
Related Files :
Chromosomes, Bacterial
Genetics
metabolism
Microbiology
Plant Disease
Plant Diseases
עוד תגיות
תוכן קשור
More details
DOI :
10.1094/PHYTO-100-3-0252
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
27080
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:28
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Scientific Publication
Sequential expression of bacterial virulence and plant defense genes during infection of tomato with clavibacter michiganensis subsp. michiganensis
100
Chalupowicz, L., Department of Plant Pathology and Weed Research, ARO, the Volcani Center, Bet Dagan, Israel, Department of Plant Sciences, Tel Aviv University, Israel
Cohen-Kandli, M., Department of Plant Pathology and Weed Research, ARO, the Volcani Center, Bet Dagan, Israel, Department of Plant Sciences, Tel Aviv University, Israel
Dror, O., Department of Plant Pathology and Weed Research, ARO, the Volcani Center, Bet Dagan, Israel
Eichenlaub R., Fakultät für Biologie, Gentechnologie/Mikrobiologie, Universität Bielefeld, Germany
Gartemann, K.-H., Fakultät für Biologie, Gentechnologie/Mikrobiologie, Universität Bielefeld, Germany
Sessa, G., Department of Plant Sciences, Tel Aviv University, Israel
Barash, I., Department of Plant Sciences, Tel Aviv University, Israel
Manulis-Sasson, S., Department of Plant Pathology and Weed Research, ARO, the Volcani Center, Bet Dagan, Israel
Sequential expression of bacterial virulence and plant defense genes during infection of tomato with clavibacter michiganensis subsp. michiganensis
Chalupowicz, L., Cohen-Kandli, M., Dror, O., Eichenlaub, R., Gartemann, K.-H., Sessa, G., Barash, I., and Manulis-Sasson, S. 2010. Sequential expression of bacterial virulence and plant defense genes during infection of tomato with Clavibacter michiganensis subsp. michiganensis. Phytopathology 100:252-261. The molecular interactions between Clavibacter michiganensis subsp. michiganensis and tomato plant were studied by following the expression of bacterial virulence and host-defense genes during early stages of infection. The C. michiganensis subsp. michiganensis genes included the plasmid-borne cellulase (celA) and the serine protease (pat-1), and the serine proteases chpC and ppaA, residing on the chp/tomA pathogenicity island (PAI). Gene expression was measured following tomato inoculation with Cmm382 (wild type), Cmm100 (lacking the plasmids pCM1 and pCM2), and Cmm27 (lacking the PAI). Transcriptional analysis revealed that celA and pat-1 were significantly induced in Cmm382 at initial 12 to 72 h, whereas chpC and ppaA were highly expressed only 96 h after inoculation. Interdependence between the expression of chromosomal and of plasmid-located genes was revealed: expression of celA and pat-1 was substantially reduced in the absence of the chp/tomA PAI, whereas chpC and ppaA expressions were reduced in the absence of the virulence plasmids. Transcription of chromosomal genes involved in cell wall degradation (i.e., pelA1, celB, xysA, and xysB), was also induced at early stages of infection. Expression of the host-defense genes, chitinase class II and pathogenesis-related protein-5 isoform was induced in the absence of the PAI at early stages of infection, suggesting that PAIlocated genes are involved in suppression of tomato basal defenses. © 2010 The American Phytopathological Society.
Scientific Publication
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