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פותח על ידי קלירמאש פתרונות בע"מ -
Understanding chilling tolerance traits using arabidopsis chilling-sensitive mutants
Year:
2012
Authors :
זולדן, דנה
;
.
פורת, רון
;
.
Volume :
Co-Authors:
Zoldan, D., Department of Postharvest Sciences of Fresh Produce, ARO, Volcani Center, P.O. Box 6, Bet Dagan, Israel
Band, R.S., Department of Environmental Horticulture, University of Florida, Gainesville, FL, United States
Guy, C.L., Department of Environmental Horticulture, University of Florida, Gainesville, FL, United States
Porat, R., Department of Postharvest Sciences of Fresh Produce, ARO, Volcani Center, P.O. Box 6, Bet Dagan, Israel
Facilitators :
From page:
159
To page:
173
(
Total pages:
15
)
Abstract:
Many plants of tropical and subtropical origin are severely damaged when exposed to chilling temperatures between 2 and 15°C. In contrast, the cruciferous plant Arabidopsis thaliana is chilling tolerant and, therefore provides an alternative model plant system for the identification of chilling tolerance traits. In this chapter, we describe physiological, biochemical, and molecular responses of Arabidopsis class 1 chilling-sensitive (chs) mutants to low temperatures. These mutants, including chs1, chs2 and chs3, are extremely chilling-sensitive and wilt and turn yellow in just a few days after transfer to low temperatures of 4–13°C. Overall, following exposure to chilling, class 1 chs mutants suffer from: (1) loss of chlorophyll and decrease in photosynthetic efficacy resulting in lack of starch accumulation, (2) damage to cellular membranes resulting in increased electrolyte leakage, and (3) accumulation of the reactive oxygen species (ROS) hydrogen peroxide (H2O2). At the molecular level, transcriptome analysis studies following exposure to 10°C for 48 h using the Affymetrix ATH1 genome array reveal remarkable changes in expression patterns of between 1,500 and 3,000 genes, which are significantly differentially expressed (p≤ 0.05 and up or down-regulated by a factor of at least 4) in chs1, chs2, and chs3 mutants compared to wild-type (WT) plants. The main functional categories of up-regulated genes by chilling include “stress,” “protein,” and “signaling,” whereas the main categories down-regulated by chilling were “photosynthesis,” “tetrapyrrole synthesis,” “carbohydrate metabolism,” “cell wall,” and “lipid metabolism”. Overall, these and other studies using Arabidopsis chilling-sensitive mutants allow the recognition of major genetic traits crucial for plant survival under chilling conditions. © Springer Science+Business Media, LLC 2012. All rights reserved.
Note:
Related Files :
arabidopsis
Biochemical response
gene mapping
Low temperature stress
physiological response
ROS
transcriptome
עוד תגיות
תוכן קשור
More details
DOI :
10.1007/978-1-4614-0815-4_7
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
פרק מתוך ספר
;
.
Language:
אנגלית
Editors' remarks:
ID:
27100
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:28
Scientific Publication
Understanding chilling tolerance traits using arabidopsis chilling-sensitive mutants
Zoldan, D., Department of Postharvest Sciences of Fresh Produce, ARO, Volcani Center, P.O. Box 6, Bet Dagan, Israel
Band, R.S., Department of Environmental Horticulture, University of Florida, Gainesville, FL, United States
Guy, C.L., Department of Environmental Horticulture, University of Florida, Gainesville, FL, United States
Porat, R., Department of Postharvest Sciences of Fresh Produce, ARO, Volcani Center, P.O. Box 6, Bet Dagan, Israel
Understanding chilling tolerance traits using arabidopsis chilling-sensitive mutants
Many plants of tropical and subtropical origin are severely damaged when exposed to chilling temperatures between 2 and 15°C. In contrast, the cruciferous plant Arabidopsis thaliana is chilling tolerant and, therefore provides an alternative model plant system for the identification of chilling tolerance traits. In this chapter, we describe physiological, biochemical, and molecular responses of Arabidopsis class 1 chilling-sensitive (chs) mutants to low temperatures. These mutants, including chs1, chs2 and chs3, are extremely chilling-sensitive and wilt and turn yellow in just a few days after transfer to low temperatures of 4–13°C. Overall, following exposure to chilling, class 1 chs mutants suffer from: (1) loss of chlorophyll and decrease in photosynthetic efficacy resulting in lack of starch accumulation, (2) damage to cellular membranes resulting in increased electrolyte leakage, and (3) accumulation of the reactive oxygen species (ROS) hydrogen peroxide (H2O2). At the molecular level, transcriptome analysis studies following exposure to 10°C for 48 h using the Affymetrix ATH1 genome array reveal remarkable changes in expression patterns of between 1,500 and 3,000 genes, which are significantly differentially expressed (p≤ 0.05 and up or down-regulated by a factor of at least 4) in chs1, chs2, and chs3 mutants compared to wild-type (WT) plants. The main functional categories of up-regulated genes by chilling include “stress,” “protein,” and “signaling,” whereas the main categories down-regulated by chilling were “photosynthesis,” “tetrapyrrole synthesis,” “carbohydrate metabolism,” “cell wall,” and “lipid metabolism”. Overall, these and other studies using Arabidopsis chilling-sensitive mutants allow the recognition of major genetic traits crucial for plant survival under chilling conditions. © Springer Science+Business Media, LLC 2012. All rights reserved.
Scientific Publication
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