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Journal of Plant Growth Regulation

Rosenwasser, S., Department of Postharvest Science of Fresh Produce, Agricultural Research Organization (ARO), The Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel, Institute of Plant Sciences and Genetics in Agriculture, The Robert H. Smith Faculty of Agriculture, Food and Environmental Quality Sciences, The Hebrew University of Jerusalem, Rehovot, Israel
Belausov, E., Department of Plant Science, Agricultural Research Organization (ARO), The Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Riov, J., Institute of Plant Sciences and Genetics in Agriculture, The Robert H. Smith Faculty of Agriculture, Food and Environmental Quality Sciences, The Hebrew University of Jerusalem, Rehovot, Israel
Holdengreber, V., Department of Plant Pathology and Weed Protection, Agricultural Research Organization (ARO), The Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Friedman, H., Department of Postharvest Science of Fresh Produce, Agricultural Research Organization (ARO), The Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel

The temporal and spatial changes in reactive oxygen species (ROS) during dark treatment of Pelargonium cuttings and the effect of gibberellic acid (GA3) on ROS levels were studied. ROS-related fluorescence was detected in mitochondria and cytoplasm of epidermal cells and in chloroplasts. By monitoring dichlorofluorescein (DCF) fluorescence, an initial decrease in ROS was observed under darkness in the epidermal cell cytoplasm and the chloroplasts, which was followed by an increase on the third day. Following 3 days under darkness, the size and the structure of the chloroplasts also changed, and they became more sensitive to illumination as judged by a higher accumulation of ROS. Pretreatment of leaves with GA3 did not prevent the structural changes in the chloroplasts, but it inhibited the increase in ROS levels in all cell compartments, including the chloroplasts. It is suggested that the inhibition of ROS increase by GA3 prevented complete disintegration of chloroplasts during dark-induced senescence and thereby enabled the maintenance of chlorophyll levels in the tissue. © 2010 Springer Science+Business Media, LLC.
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הספר "אוצר וולקני"
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תנאי שימוש
Gibberellic acid (GA3) inhibits ROS increase in chloroplasts during dark-induced senescence of pelargonium cuttings
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Rosenwasser, S., Department of Postharvest Science of Fresh Produce, Agricultural Research Organization (ARO), The Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel, Institute of Plant Sciences and Genetics in Agriculture, The Robert H. Smith Faculty of Agriculture, Food and Environmental Quality Sciences, The Hebrew University of Jerusalem, Rehovot, Israel
Belausov, E., Department of Plant Science, Agricultural Research Organization (ARO), The Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Riov, J., Institute of Plant Sciences and Genetics in Agriculture, The Robert H. Smith Faculty of Agriculture, Food and Environmental Quality Sciences, The Hebrew University of Jerusalem, Rehovot, Israel
Holdengreber, V., Department of Plant Pathology and Weed Protection, Agricultural Research Organization (ARO), The Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Friedman, H., Department of Postharvest Science of Fresh Produce, Agricultural Research Organization (ARO), The Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel

Gibberellic acid (GA3) inhibits ROS increase in chloroplasts during dark-induced senescence of pelargonium cuttings
The temporal and spatial changes in reactive oxygen species (ROS) during dark treatment of Pelargonium cuttings and the effect of gibberellic acid (GA3) on ROS levels were studied. ROS-related fluorescence was detected in mitochondria and cytoplasm of epidermal cells and in chloroplasts. By monitoring dichlorofluorescein (DCF) fluorescence, an initial decrease in ROS was observed under darkness in the epidermal cell cytoplasm and the chloroplasts, which was followed by an increase on the third day. Following 3 days under darkness, the size and the structure of the chloroplasts also changed, and they became more sensitive to illumination as judged by a higher accumulation of ROS. Pretreatment of leaves with GA3 did not prevent the structural changes in the chloroplasts, but it inhibited the increase in ROS levels in all cell compartments, including the chloroplasts. It is suggested that the inhibition of ROS increase by GA3 prevented complete disintegration of chloroplasts during dark-induced senescence and thereby enabled the maintenance of chlorophyll levels in the tissue. © 2010 Springer Science+Business Media, LLC.
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