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פותח על ידי קלירמאש פתרונות בע"מ -
High-throughput marker discovery in melon using a self-designed oligo microarray
Year:
2010
Source of publication :
BMC Genomics
Authors :
אוליאל, שי
;
.
אופיר, רון
;
.
אשד, רוית
;
.
בורגר, יוסף
;
.
הראל-ביז'ה, רותם
;
.
פורטנוי, ויטלי
;
.
צורי, גליל
;
.
קציר, נורית
;
.
שרמן, עמיר
;
.
Volume :
11
Co-Authors:
Ophir, R., Genomic Unit, Plant Sciences Institute, Volcani Center, Agricultural Research Organization, PO Box 6, Bet Dagan 50250, Israel
Eshed, R., Genomic Unit, Plant Sciences Institute, Volcani Center, Agricultural Research Organization, PO Box 6, Bet Dagan 50250, Israel
Harel-Beja, R., Department of Vegetable Crops, Newe Ya'ar Research Center, Agricultural Research Organization, PO Box 1021, Ramat Ishai 30095, Israel
Tzuri, G., Department of Vegetable Crops, Newe Ya'ar Research Center, Agricultural Research Organization, PO Box 1021, Ramat Ishai 30095, Israel
Portnoy, V., Department of Vegetable Crops, Newe Ya'ar Research Center, Agricultural Research Organization, PO Box 1021, Ramat Ishai 30095, Israel
Burger, Y., Department of Vegetable Crops, Newe Ya'ar Research Center, Agricultural Research Organization, PO Box 1021, Ramat Ishai 30095, Israel
Uliel, S., Genomic Unit, Plant Sciences Institute, Volcani Center, Agricultural Research Organization, PO Box 6, Bet Dagan 50250, Israel
Katzir, N., Department of Vegetable Crops, Newe Ya'ar Research Center, Agricultural Research Organization, PO Box 1021, Ramat Ishai 30095, Israel
Sherman, A., Genomic Unit, Plant Sciences Institute, Volcani Center, Agricultural Research Organization, PO Box 6, Bet Dagan 50250, Israel
Facilitators :
From page:
To page:
(
Total pages:
1
)
Abstract:
Background: Genetic maps constitute the basis of breeding programs for many agricultural organisms. The creation of these maps is dependent on marker discovery. Melon, among other crops, is still lagging in genomic resources, limiting the ability to discover new markers in a high-throughput fashion. One of the methods used to search for molecular markers is DNA hybridization to microarrays. Microarray hybridization of DNA from different accessions can reveal differences between them--single-feature polymorphisms (SFPs). These SFPs can be used as markers for breeding purposes, or they can be converted to conventional markers by sequencing. This method has been utilized in a few different plants to discover genetic variation, using Affymetrix arrays that exist for only a few organisms. We applied this approach with some modifications for marker discovery in melon.Results: Using a custom-designed oligonucleotide microarray based on a partial EST collection of melon, we discovered 6184 putative SFPs between the parents of our mapping population. Validation by sequencing of 245 SFPs from the two parents showed a sensitivity of around 79%. Most SFPs (81%) contained single-nucleotide polymorphisms. Testing the SFPs on another mapping population of melon confirmed that many of them are conserved.Conclusion: Thousands of new SFPs that can be used for genetic mapping and molecular-assisted breeding in melon were discovered using a custom-designed oligo microarray. A portion of these SFPs are conserved and can be used in different breeding populations. Although improvement of the discovery rate is still needed, this approach is applicable to many agricultural systems with limited genomic resources. © 2010 Ophir et al; licensee BioMed Central Ltd.
Note:
Related Files :
chromosome map
chromosome mapping
Cucurbitaceae
gene mapping
Genetics
Plant Breeding
sequence analysis
עוד תגיות
תוכן קשור
More details
DOI :
10.1186/1471-2164-11-269
Article number:
269
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
27182
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:28
Scientific Publication
High-throughput marker discovery in melon using a self-designed oligo microarray
11
Ophir, R., Genomic Unit, Plant Sciences Institute, Volcani Center, Agricultural Research Organization, PO Box 6, Bet Dagan 50250, Israel
Eshed, R., Genomic Unit, Plant Sciences Institute, Volcani Center, Agricultural Research Organization, PO Box 6, Bet Dagan 50250, Israel
Harel-Beja, R., Department of Vegetable Crops, Newe Ya'ar Research Center, Agricultural Research Organization, PO Box 1021, Ramat Ishai 30095, Israel
Tzuri, G., Department of Vegetable Crops, Newe Ya'ar Research Center, Agricultural Research Organization, PO Box 1021, Ramat Ishai 30095, Israel
Portnoy, V., Department of Vegetable Crops, Newe Ya'ar Research Center, Agricultural Research Organization, PO Box 1021, Ramat Ishai 30095, Israel
Burger, Y., Department of Vegetable Crops, Newe Ya'ar Research Center, Agricultural Research Organization, PO Box 1021, Ramat Ishai 30095, Israel
Uliel, S., Genomic Unit, Plant Sciences Institute, Volcani Center, Agricultural Research Organization, PO Box 6, Bet Dagan 50250, Israel
Katzir, N., Department of Vegetable Crops, Newe Ya'ar Research Center, Agricultural Research Organization, PO Box 1021, Ramat Ishai 30095, Israel
Sherman, A., Genomic Unit, Plant Sciences Institute, Volcani Center, Agricultural Research Organization, PO Box 6, Bet Dagan 50250, Israel
High-throughput marker discovery in melon using a self-designed oligo microarray
Background: Genetic maps constitute the basis of breeding programs for many agricultural organisms. The creation of these maps is dependent on marker discovery. Melon, among other crops, is still lagging in genomic resources, limiting the ability to discover new markers in a high-throughput fashion. One of the methods used to search for molecular markers is DNA hybridization to microarrays. Microarray hybridization of DNA from different accessions can reveal differences between them--single-feature polymorphisms (SFPs). These SFPs can be used as markers for breeding purposes, or they can be converted to conventional markers by sequencing. This method has been utilized in a few different plants to discover genetic variation, using Affymetrix arrays that exist for only a few organisms. We applied this approach with some modifications for marker discovery in melon.Results: Using a custom-designed oligonucleotide microarray based on a partial EST collection of melon, we discovered 6184 putative SFPs between the parents of our mapping population. Validation by sequencing of 245 SFPs from the two parents showed a sensitivity of around 79%. Most SFPs (81%) contained single-nucleotide polymorphisms. Testing the SFPs on another mapping population of melon confirmed that many of them are conserved.Conclusion: Thousands of new SFPs that can be used for genetic mapping and molecular-assisted breeding in melon were discovered using a custom-designed oligo microarray. A portion of these SFPs are conserved and can be used in different breeding populations. Although improvement of the discovery rate is still needed, this approach is applicable to many agricultural systems with limited genomic resources. © 2010 Ophir et al; licensee BioMed Central Ltd.
Scientific Publication
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