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קהילה:
אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
Cloning, expression, and tissue localization of the calcium-sensing receptor in chicken (Gallus domesticus)
Year:
1997
Authors :
הורויץ, שמואל (בעלי חיים)
;
.
פינס, מרק
;
.
פרידמן-עינת, מרים
;
.
Volume :
273
Co-Authors:
Diaz, R., Endocrine-Hypertension Division, Department of Medicine, Brigham and Women's Hospital, Boston, MA 02115, United States, Endocrine Division, Harvard Medical School, Children's Hospital, Boston, MA 02115, United States, Endocrine-Hypertension Division, Brigham and Women's Hospital, 221 Longwood Ave, Boston, MA 02115, United States
Hurwitz, S., Agricultural Research Organization, Volcani Center, Bet Dagan, 50250, Israel
Chattopadhyay, N., Endocrine-Hypertension Division, Department of Medicine, Brigham and Women's Hospital, Boston, MA 02115, United States
Pines, M., Endocrine Division, Harvard Medical School, Children's Hospital, Boston, MA 02115, United States
Yang, Y., Renal Division, Department of Medicine, Brigham and Women's Hospital, Boston, MA 02115, United States
Kifor, O., Endocrine-Hypertension Division, Department of Medicine, Brigham and Women's Hospital, Boston, MA 02115, United States
Einat, M.S., Agricultural Research Organization, Volcani Center, Bet Dagan, 50250, Israel
Butters, R., Endocrine-Hypertension Division, Department of Medicine, Brigham and Women's Hospital, Boston, MA 02115, United States
Hebert, S.C., Renal Division, Department of Medicine, Brigham and Women's Hospital, Boston, MA 02115, United States
Brown, E.M., Endocrine-Hypertension Division, Department of Medicine, Brigham and Women's Hospital, Boston, MA 02115, United States
Facilitators :
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0
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0
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Total pages:
1
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Abstract:
In previous studies, we characterized an extracellular Ca 2+ (Ca(o)/ 2+)-sensing receptor (CaR) that plays a central role in regulating parathyroid hormone secretion in mammals by sensing Ca(o)/ 2+. In the present study, we have cloned and characterized the chicken (Gallus domesticus) homolog of the CaR. The chicken parathyroid CaR shares a high degree of homology (84% amino acid identity) with the human CaR and displays a similar topology. Moreover, amino acid residues where mutations cause disorders of Ca(o)/ 2+-sensing in the human CaR share the wild-type human sequence in the chicken CaR. However, a single region in the extracellular domain of the chicken CaR differs substantially from its mammalian homologs. Xenopus laevis oocytes injected with chicken CaR cRNA respond to elevated ambient levels of Ca(o)/ 2+, extracellular Mg 2+, or extracellular Gd 3+ with the characteristic activation of inositol triphosphate-dependent, intracellular Ca 2+-induced Cl - currents elicited by mammalian CaRs as well as by G protein-linked receptors coupled to activation of phospholipase C. By in situ hybridization, clusters of cells in chicken parathyroid glands were shown to express CaR messenger RNA. Northern analysis and immunohistochemistry demonstrated expression of receptor transcripts and/or protein in kidney tubules and intestine as well as in brain. The close conservation of the amino acid sequence of the chicken CaR with its mammalian homologs as well as its similar tissue distribution suggest that the receptor may also play an important role in avian calcium homeostasis.
Note:
Related Files :
animal cell
Animals
animal tissue
Base Sequence
Chickens
Female
Parathyroid Glands
עוד תגיות
תוכן קשור
More details
DOI :
Article number:
0
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
27228
Last updated date:
21/08/2022 07:45
Creation date:
17/04/2018 00:29
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Scientific Publication
Cloning, expression, and tissue localization of the calcium-sensing receptor in chicken (Gallus domesticus)
273
Diaz, R., Endocrine-Hypertension Division, Department of Medicine, Brigham and Women's Hospital, Boston, MA 02115, United States, Endocrine Division, Harvard Medical School, Children's Hospital, Boston, MA 02115, United States, Endocrine-Hypertension Division, Brigham and Women's Hospital, 221 Longwood Ave, Boston, MA 02115, United States
Hurwitz, S., Agricultural Research Organization, Volcani Center, Bet Dagan, 50250, Israel
Chattopadhyay, N., Endocrine-Hypertension Division, Department of Medicine, Brigham and Women's Hospital, Boston, MA 02115, United States
Pines, M., Endocrine Division, Harvard Medical School, Children's Hospital, Boston, MA 02115, United States
Yang, Y., Renal Division, Department of Medicine, Brigham and Women's Hospital, Boston, MA 02115, United States
Kifor, O., Endocrine-Hypertension Division, Department of Medicine, Brigham and Women's Hospital, Boston, MA 02115, United States
Einat, M.S., Agricultural Research Organization, Volcani Center, Bet Dagan, 50250, Israel
Butters, R., Endocrine-Hypertension Division, Department of Medicine, Brigham and Women's Hospital, Boston, MA 02115, United States
Hebert, S.C., Renal Division, Department of Medicine, Brigham and Women's Hospital, Boston, MA 02115, United States
Brown, E.M., Endocrine-Hypertension Division, Department of Medicine, Brigham and Women's Hospital, Boston, MA 02115, United States
Cloning, expression, and tissue localization of the calcium-sensing receptor in chicken (Gallus domesticus)
In previous studies, we characterized an extracellular Ca 2+ (Ca(o)/ 2+)-sensing receptor (CaR) that plays a central role in regulating parathyroid hormone secretion in mammals by sensing Ca(o)/ 2+. In the present study, we have cloned and characterized the chicken (Gallus domesticus) homolog of the CaR. The chicken parathyroid CaR shares a high degree of homology (84% amino acid identity) with the human CaR and displays a similar topology. Moreover, amino acid residues where mutations cause disorders of Ca(o)/ 2+-sensing in the human CaR share the wild-type human sequence in the chicken CaR. However, a single region in the extracellular domain of the chicken CaR differs substantially from its mammalian homologs. Xenopus laevis oocytes injected with chicken CaR cRNA respond to elevated ambient levels of Ca(o)/ 2+, extracellular Mg 2+, or extracellular Gd 3+ with the characteristic activation of inositol triphosphate-dependent, intracellular Ca 2+-induced Cl - currents elicited by mammalian CaRs as well as by G protein-linked receptors coupled to activation of phospholipase C. By in situ hybridization, clusters of cells in chicken parathyroid glands were shown to express CaR messenger RNA. Northern analysis and immunohistochemistry demonstrated expression of receptor transcripts and/or protein in kidney tubules and intestine as well as in brain. The close conservation of the amino acid sequence of the chicken CaR with its mammalian homologs as well as its similar tissue distribution suggest that the receptor may also play an important role in avian calcium homeostasis.
Scientific Publication
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