נגישות
menu      
חיפוש מתקדם
תחביר
חפש...
הספר "אוצר וולקני"
אודות
תנאי שימוש
ניהול
קהילה:
אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
Nutrient transport in the small intestine: Na+,K +-ATPase expression and activity in the small intestine of the chicken as influenced by dietary sodium
Year:
2003
Source of publication :
Poultry Science
Authors :
מבג'יש, סמיר
;
.
Volume :
82
Co-Authors:
Gal-Garber, O., Department of Animal Sciences, Fac. Agric. Food Environ. Qual. Sci., Hebrew University of Jerusalem, P.O. Box 12, Rehovot, 76100, Israel
Mabjeesh, S.J., Department of Animal Sciences, Fac. Agric. Food Environ. Qual. Sci., Hebrew University of Jerusalem, P.O. Box 12, Rehovot, 76100, Israel
Sklan, D., Department of Animal Sciences, Fac. Agric. Food Environ. Qual. Sci., Hebrew University of Jerusalem, P.O. Box 12, Rehovot, 76100, Israel
Uni, Z., Department of Animal Sciences, Fac. Agric. Food Environ. Qual. Sci., Hebrew University of Jerusalem, P.O. Box 12, Rehovot, 76100, Israel
Facilitators :
From page:
1127
To page:
1133
(
Total pages:
7
)
Abstract:
The Na+-K+-ATPase, localized in the basolateral membrane of enterocytes plays a major role in nutrient transport in the small intestine by transferring K+ ions into- and Na+ out of the cell. Within the enterocyte, homeostasis is maintained by active exclusion of Na from the cell by the Na+, K+-adenosine triphosphatase (ATPase) or sodium pump. Because much of the intestinal nutrient transport is by Na cotransporters, Na+, K+-ATPase may be used to evaluate nutrient uptake. In this study, nutrient transport was evaluated by determining expression and activity of Na+-K+-ATPase in the jejunum of chicks fed diets with different concentrations of Na. Expression of the chicken Na+-K+-ATPase gene was examined following isolation of an 1,140 bp cDNA fragment of the α-subunit using a reverse transcription (RT)-PCR reaction with specific primers. This fragment was sequenced and showed 95 to 98% homology with the mammalian α-subunit of the Na+-K+-ATPase genes. This cDNA fragment was used as a specific probe in Northern blot hybridization for determination of expression in the chicken jejunum. Expression of mRNA of Na+-K+-ATPase was enhanced at low dietary Na but was unchanged at high dietary Na concentrations. In contrast, activity of the enzyme was low with low dietary Na and unchanged at high dietary Na. The Vmax of the Na+-K +-ATPase was unchanged, but affinity was altered by dietary Na concentrations. Thus, determination of expression and activity of intestinal Na+-K+-ATPase allows clearer understanding of changes in intestinal uptake due to dietary Na.
Note:
Related Files :
Animal
Animals
Blood
gene expression
Genetics
Mammalia
metabolism
molecular genetics
עוד תגיות
תוכן קשור
More details
DOI :
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
27236
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:29
You may also be interested in
Scientific Publication
Nutrient transport in the small intestine: Na+,K +-ATPase expression and activity in the small intestine of the chicken as influenced by dietary sodium
82
Gal-Garber, O., Department of Animal Sciences, Fac. Agric. Food Environ. Qual. Sci., Hebrew University of Jerusalem, P.O. Box 12, Rehovot, 76100, Israel
Mabjeesh, S.J., Department of Animal Sciences, Fac. Agric. Food Environ. Qual. Sci., Hebrew University of Jerusalem, P.O. Box 12, Rehovot, 76100, Israel
Sklan, D., Department of Animal Sciences, Fac. Agric. Food Environ. Qual. Sci., Hebrew University of Jerusalem, P.O. Box 12, Rehovot, 76100, Israel
Uni, Z., Department of Animal Sciences, Fac. Agric. Food Environ. Qual. Sci., Hebrew University of Jerusalem, P.O. Box 12, Rehovot, 76100, Israel
Nutrient transport in the small intestine: Na+,K +-ATPase expression and activity in the small intestine of the chicken as influenced by dietary sodium
The Na+-K+-ATPase, localized in the basolateral membrane of enterocytes plays a major role in nutrient transport in the small intestine by transferring K+ ions into- and Na+ out of the cell. Within the enterocyte, homeostasis is maintained by active exclusion of Na from the cell by the Na+, K+-adenosine triphosphatase (ATPase) or sodium pump. Because much of the intestinal nutrient transport is by Na cotransporters, Na+, K+-ATPase may be used to evaluate nutrient uptake. In this study, nutrient transport was evaluated by determining expression and activity of Na+-K+-ATPase in the jejunum of chicks fed diets with different concentrations of Na. Expression of the chicken Na+-K+-ATPase gene was examined following isolation of an 1,140 bp cDNA fragment of the α-subunit using a reverse transcription (RT)-PCR reaction with specific primers. This fragment was sequenced and showed 95 to 98% homology with the mammalian α-subunit of the Na+-K+-ATPase genes. This cDNA fragment was used as a specific probe in Northern blot hybridization for determination of expression in the chicken jejunum. Expression of mRNA of Na+-K+-ATPase was enhanced at low dietary Na but was unchanged at high dietary Na concentrations. In contrast, activity of the enzyme was low with low dietary Na and unchanged at high dietary Na. The Vmax of the Na+-K +-ATPase was unchanged, but affinity was altered by dietary Na concentrations. Thus, determination of expression and activity of intestinal Na+-K+-ATPase allows clearer understanding of changes in intestinal uptake due to dietary Na.
Scientific Publication
You may also be interested in