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פותח על ידי קלירמאש פתרונות בע"מ -
Differential Regulation of the Rat Phosphoenolpyruvate Carboxykinase Gene Expression in Several Tissues of Transgenic Mice
Year:
1992
Source of publication :
Molecular and Cellular Biology
Authors :
שני, משה
;
.
Volume :
12
Co-Authors:
Eisenberger, C.L., Dept. of Developmental Biochemistry, Institute of Biochemistry, Hebrew Univ. Hadassah Medical School, Jerusalem 91010, Israel
Nechushtan, H., Dept. of Developmental Biochemistry, Institute of Biochemistry, Hebrew Univ. Hadassah Medical School, Jerusalem 91010, Israel
Cohen, H., Dept. of Developmental Biochemistry, Institute of Biochemistry, Hebrew Univ. Hadassah Medical School, Jerusalem 91010, Israel
Shani, M., Institute of Animal Sciences, Volcani Center, Bet Dagan 50250, Israel
Reshef, L., Dept. of Developmental Biochemistry, Institute of Biochemistry, Hebrew Univ. Hadassah Medical School, Jerusalem 91010, Israel
Facilitators :
From page:
1396
To page:
1403
(
Total pages:
8
)
Abstract:
The selective expression of a unique copy gene in several mammalian tissues has been approached by studying the regulatory sequences needed to control expression of the rat phosphoenolpyruvate carboxykinase (PEPCK) gene in transgenic mice. A transgene containing the entire PEPCK gene, including 2.2 kb of the 5′-flanking region and 0.5 kb of the 3′-flanking region, exhibits tissue-specific expression in the liver, kidney, and adipose tissue, as well as the hormonal and developmental regulation inherent to endogenous gene expression. Deletions of the 5′-flanking region of the gene have shown the need for sequences downstream of position -540 of the PEPCK gene for expression in the liver and sequences downstream of position -362 for expression in the kidney. Additional sequences upstream of position -540 (up to -2200) are required for expression in adipose tissue. In addition, the region containing the glucocorticoid-responsive elements of the gene used by the kidney was identified. This same sequence was found to be needed specifically for developmental regulation of gene expression in the kidney and, together with upstream sequences, in the intestine. The apparently distinct sequence requirements in the various tissues indicate that the tissues use different mechanisms for expression of the same gene.
Note:
Related Files :
adipose tissue
aging
Animal
animal cell
animal tissue
liver
Male
Mammalia
mice
עוד תגיות
תוכן קשור
More details
DOI :
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
27257
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:29
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Differential Regulation of the Rat Phosphoenolpyruvate Carboxykinase Gene Expression in Several Tissues of Transgenic Mice
12
Eisenberger, C.L., Dept. of Developmental Biochemistry, Institute of Biochemistry, Hebrew Univ. Hadassah Medical School, Jerusalem 91010, Israel
Nechushtan, H., Dept. of Developmental Biochemistry, Institute of Biochemistry, Hebrew Univ. Hadassah Medical School, Jerusalem 91010, Israel
Cohen, H., Dept. of Developmental Biochemistry, Institute of Biochemistry, Hebrew Univ. Hadassah Medical School, Jerusalem 91010, Israel
Shani, M., Institute of Animal Sciences, Volcani Center, Bet Dagan 50250, Israel
Reshef, L., Dept. of Developmental Biochemistry, Institute of Biochemistry, Hebrew Univ. Hadassah Medical School, Jerusalem 91010, Israel
Differential Regulation of the Rat Phosphoenolpyruvate Carboxykinase Gene Expression in Several Tissues of Transgenic Mice
The selective expression of a unique copy gene in several mammalian tissues has been approached by studying the regulatory sequences needed to control expression of the rat phosphoenolpyruvate carboxykinase (PEPCK) gene in transgenic mice. A transgene containing the entire PEPCK gene, including 2.2 kb of the 5′-flanking region and 0.5 kb of the 3′-flanking region, exhibits tissue-specific expression in the liver, kidney, and adipose tissue, as well as the hormonal and developmental regulation inherent to endogenous gene expression. Deletions of the 5′-flanking region of the gene have shown the need for sequences downstream of position -540 of the PEPCK gene for expression in the liver and sequences downstream of position -362 for expression in the kidney. Additional sequences upstream of position -540 (up to -2200) are required for expression in adipose tissue. In addition, the region containing the glucocorticoid-responsive elements of the gene used by the kidney was identified. This same sequence was found to be needed specifically for developmental regulation of gene expression in the kidney and, together with upstream sequences, in the intestine. The apparently distinct sequence requirements in the various tissues indicate that the tissues use different mechanisms for expression of the same gene.
Scientific Publication
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