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פותח על ידי קלירמאש פתרונות בע"מ -
Double-stranded conformation polymorphism of heterologous RNA transcripts and its use for virus strain differentiation
Year:
1999
Source of publication :
Plant Pathology
Authors :
מסלנין, לודמילה
;
.
רוזנר, אריה
;
.
שפיגל, שרה
;
.
Volume :
48
Co-Authors:
Rosner, A., Department of Virology, Volcani Center, ARO, Bet Dagan 50250, Israel
Maslenin, L., Department of Virology, Volcani Center, ARO, Bet Dagan 50250, Israel
Spiegel, S., Department of Virology, Volcani Center, ARO, Bet Dagan 50250, Israel
Facilitators :
From page:
235
To page:
239
(
Total pages:
5
)
Abstract:
Polymorphism of heterologous duplexes of RNA transcripts of PNRSV isolates was demonstrated. Complementary RNA trancripts were copied from PCR products of PNRSV isolates, to which the T7 RNA promoter was added on either the 3' or 5'-ends. Heterologous duplexes made from complementary RNA transcripts originating from several virus isolates differed in their electrophoretic mobilities. This polymorphism is hypothesized to be conferred by conformational variations among the transcript duplexes, and was termed by us 'double-stranded transcript conformation polymorphism (ds-TCP)'. This method may serve as an additional tool in virus strain differentiation.
Note:
Related Files :
Conformation polymorphism
Ilarviruses
Prunus necrotic ringspot virus
Strain differentiation
viral genetics
virus detection
virus RNA
עוד תגיות
תוכן קשור
More details
DOI :
10.1046/j.1365-3059.1999.00335.x
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
27258
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:29
You may also be interested in
Scientific Publication
Double-stranded conformation polymorphism of heterologous RNA transcripts and its use for virus strain differentiation
48
Rosner, A., Department of Virology, Volcani Center, ARO, Bet Dagan 50250, Israel
Maslenin, L., Department of Virology, Volcani Center, ARO, Bet Dagan 50250, Israel
Spiegel, S., Department of Virology, Volcani Center, ARO, Bet Dagan 50250, Israel
Double-stranded conformation polymorphism of heterologous RNA transcripts and its use for virus strain differentiation
Polymorphism of heterologous duplexes of RNA transcripts of PNRSV isolates was demonstrated. Complementary RNA trancripts were copied from PCR products of PNRSV isolates, to which the T7 RNA promoter was added on either the 3' or 5'-ends. Heterologous duplexes made from complementary RNA transcripts originating from several virus isolates differed in their electrophoretic mobilities. This polymorphism is hypothesized to be conferred by conformational variations among the transcript duplexes, and was termed by us 'double-stranded transcript conformation polymorphism (ds-TCP)'. This method may serve as an additional tool in virus strain differentiation.
Scientific Publication
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