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פותח על ידי קלירמאש פתרונות בע"מ -
Isolation and Functional Characterization of Carotenoid Cleavage Dioxygenase-1 from Laurus nobilis L. (Bay Laurel) Fruits
Year:
2015
Authors :
אבדאח, מוופק
;
.
איזיקסון, טל
;
.
בר, עינת
;
.
דוידוביץ'-רקנאטי, רחל
;
.
יאהיה, מוסעב
;
.
לוינסון, אפרים
;
.
מרזוק, סלי
;
.
Volume :
63
Co-Authors:
Yahyaa, M., NeweYaar Research Center, Agriculture Research Organization, P.O. Box 1021, Ramat Yishay, Israel
Berim, A., Institute of Biological Chemistry, Washington State University, P.O. Box 646340, Pullman, Washington, United States
Isaacson, T., NeweYaar Research Center, Agriculture Research Organization, P.O. Box 1021, Ramat Yishay, Israel
Marzouk, S., NeweYaar Research Center, Agriculture Research Organization, P.O. Box 1021, Ramat Yishay, Israel
Bar, E., NeweYaar Research Center, Agriculture Research Organization, P.O. Box 1021, Ramat Yishay, Israel
Davidovich-Rikanati, R., NeweYaar Research Center, Agriculture Research Organization, P.O. Box 1021, Ramat Yishay, Israel
Lewinsohn, E., NeweYaar Research Center, Agriculture Research Organization, P.O. Box 1021, Ramat Yishay, Israel
Ibdah, M., NeweYaar Research Center, Agriculture Research Organization, P.O. Box 1021, Ramat Yishay, Israel
Facilitators :
From page:
8275
To page:
8282
(
Total pages:
8
)
Abstract:
Bay laurel (Laurus nobilis L.) is an agriculturally important tree used in food, drugs, and the cosmetics industry. Many of the health beneficial properties of bay laurel are due to volatile terpene metabolites that they contain, including various norisoprenoids. Despite their importance, little is known about the norisoprenoid biosynthesis in Laurus nobilis fruits. We found that the volatile norisoprenoids 6-methyl-5-hepten-2-one, pseudoionone, and β-ionone accumulated in Laurus nobilis fruits in a pattern reflecting their carotenoid content. A full-length cDNA encoding a potential carotenoid cleavage dioxygenase (LnCCD1) was isolated. The LnCCD1 gene was overexpressed in Escherichia coli, and recombinant protein was assayed for its cleavage activity with an array of carotenoid substrates. The LnCCD1 protein was able to cleave a variety of carotenoids at the 9,10 (9′,10′) and 5,6 (5′,6′) positions to produce 6-methyl-5-hepten-2-one, pseudoionone, β-ionone, and α-ionone. Our results suggest a role for LnCCD1 in Laurus nobilis fruit flavor biosynthesis. © 2015 American Chemical Society.
Note:
Related Files :
Biochemistry
gene expression
Genetics
Laurus nobilis
taste
עוד תגיות
תוכן קשור
More details
DOI :
10.1021/acs.jafc.5b02941
Article number:
0
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
27283
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:29
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Scientific Publication
Isolation and Functional Characterization of Carotenoid Cleavage Dioxygenase-1 from Laurus nobilis L. (Bay Laurel) Fruits
63
Yahyaa, M., NeweYaar Research Center, Agriculture Research Organization, P.O. Box 1021, Ramat Yishay, Israel
Berim, A., Institute of Biological Chemistry, Washington State University, P.O. Box 646340, Pullman, Washington, United States
Isaacson, T., NeweYaar Research Center, Agriculture Research Organization, P.O. Box 1021, Ramat Yishay, Israel
Marzouk, S., NeweYaar Research Center, Agriculture Research Organization, P.O. Box 1021, Ramat Yishay, Israel
Bar, E., NeweYaar Research Center, Agriculture Research Organization, P.O. Box 1021, Ramat Yishay, Israel
Davidovich-Rikanati, R., NeweYaar Research Center, Agriculture Research Organization, P.O. Box 1021, Ramat Yishay, Israel
Lewinsohn, E., NeweYaar Research Center, Agriculture Research Organization, P.O. Box 1021, Ramat Yishay, Israel
Ibdah, M., NeweYaar Research Center, Agriculture Research Organization, P.O. Box 1021, Ramat Yishay, Israel
Isolation and Functional Characterization of Carotenoid Cleavage Dioxygenase-1 from Laurus nobilis L. (Bay Laurel) Fruits
Bay laurel (Laurus nobilis L.) is an agriculturally important tree used in food, drugs, and the cosmetics industry. Many of the health beneficial properties of bay laurel are due to volatile terpene metabolites that they contain, including various norisoprenoids. Despite their importance, little is known about the norisoprenoid biosynthesis in Laurus nobilis fruits. We found that the volatile norisoprenoids 6-methyl-5-hepten-2-one, pseudoionone, and β-ionone accumulated in Laurus nobilis fruits in a pattern reflecting their carotenoid content. A full-length cDNA encoding a potential carotenoid cleavage dioxygenase (LnCCD1) was isolated. The LnCCD1 gene was overexpressed in Escherichia coli, and recombinant protein was assayed for its cleavage activity with an array of carotenoid substrates. The LnCCD1 protein was able to cleave a variety of carotenoids at the 9,10 (9′,10′) and 5,6 (5′,6′) positions to produce 6-methyl-5-hepten-2-one, pseudoionone, β-ionone, and α-ionone. Our results suggest a role for LnCCD1 in Laurus nobilis fruit flavor biosynthesis. © 2015 American Chemical Society.
Scientific Publication
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