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פותח על ידי קלירמאש פתרונות בע"מ -
Dynamic changes in DNA methylation during thermal control establishment affect CREB binding to the brain-derived neurotrophic factor promoter
Year:
2008
Source of publication :
European Journal of Neuroscience
Authors :
יוסיפוף, מאיה
;
.
מאירי, נעם
;
.
קיסיליוק, טטיאנה
;
.
Volume :
28
Co-Authors:
Yossifoff, M., Institute of Animal Science, ARO, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Kisliouk, T., Institute of Animal Science, ARO, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Meiri, N., Institute of Animal Science, ARO, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Facilitators :
From page:
2267
To page:
2277
(
Total pages:
11
)
Abstract:
Thermal control establishment develops during a critical period by alterations in cellular properties in the frontal hypothalamus. These alterations may be modulated by the epigenetic code that determines the repertoire of transcribed proteins. Here we demonstrate transient changes in the expression of brain-derived neurotrophic factor (Bdnf) during both thermal conditioning and re-exposure of conditioned chicks to heat stress, relative to their age-matched naive counterparts. These changes coincide with changes in CpG methylation pattern in the avian Bdnf promoter region. Reduction in methylation during heat conditioning was observed at a cAMP response element-binding (CREB) site which coincided with both elevation in phospho-CREB levels and its binding to the Bdnf promoter. At the same time, an increase in methylation was observed at two other CpG sites, accompanied by elevation of the DNA methyltransferase 3a (DNMT3a) expression. DNMT3a was also found to bind to the two elevated methyl CpG sites, but not to the CREB binding site. These data suggest that complex and dynamic changes in DNA methylation are involved in the regulation of Bdnf expression during thermotolerance acquisition. © The Authors (2008).
Note:
Related Files :
animal experiment
Animals
epigenetics
heat stress
heat tolerance
Male
עוד תגיות
תוכן קשור
More details
DOI :
10.1111/j.1460-9568.2008.06532.x
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
27300
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:29
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Scientific Publication
Dynamic changes in DNA methylation during thermal control establishment affect CREB binding to the brain-derived neurotrophic factor promoter
28
Yossifoff, M., Institute of Animal Science, ARO, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Kisliouk, T., Institute of Animal Science, ARO, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Meiri, N., Institute of Animal Science, ARO, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Dynamic changes in DNA methylation during thermal control establishment affect CREB binding to the brain-derived neurotrophic factor promoter
Thermal control establishment develops during a critical period by alterations in cellular properties in the frontal hypothalamus. These alterations may be modulated by the epigenetic code that determines the repertoire of transcribed proteins. Here we demonstrate transient changes in the expression of brain-derived neurotrophic factor (Bdnf) during both thermal conditioning and re-exposure of conditioned chicks to heat stress, relative to their age-matched naive counterparts. These changes coincide with changes in CpG methylation pattern in the avian Bdnf promoter region. Reduction in methylation during heat conditioning was observed at a cAMP response element-binding (CREB) site which coincided with both elevation in phospho-CREB levels and its binding to the Bdnf promoter. At the same time, an increase in methylation was observed at two other CpG sites, accompanied by elevation of the DNA methyltransferase 3a (DNMT3a) expression. DNMT3a was also found to bind to the two elevated methyl CpG sites, but not to the CREB binding site. These data suggest that complex and dynamic changes in DNA methylation are involved in the regulation of Bdnf expression during thermotolerance acquisition. © The Authors (2008).
Scientific Publication
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