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פותח על ידי קלירמאש פתרונות בע"מ -
S-Linalool synthase activity in developing fruit of the columnar cactus koubo [Cereus peruvianus (L.) Miller]
Year:
2004
Source of publication :
Plant Science
Authors :
בר, עינת
;
.
גולן, עינב
;
.
לוינסון, אפרים
;
.
לרקוב, אולגה
;
.
רביד, עוזי
;
.
Volume :
167
Co-Authors:
Sitrit, Y., Institutes for Applied Research, Ben-Gurion University of the Negev, P.O. Box 653, Beer-Sheva 84105, I., Israel
Ninio, R., Institutes for Applied Research, Ben-Gurion University of the Negev, P.O. Box 653, Beer-Sheva 84105, I., Israel, Department of Life Sciences, Ben-Gurion University of the Negev, P.O. Box 653, Beer-Sheva 84105, I., Israel
Bar, E., Department of Vegetable Crops, Agricultural Research Organization, Newe Ya'ar Res. Ctr., P.O. B., Israel
Golan, E., Department of Vegetable Crops, Agricultural Research Organization, Newe Ya'ar Res. Ctr., P.O. B., Israel
Larkov, O., Department of Vegetable Crops, Agricultural Research Organization, Newe Ya'ar Res. Ctr., P.O. B., Israel
Ravid, U., Department of Vegetable Crops, Agricultural Research Organization, Newe Ya'ar Res. Ctr., P.O. B., Israel
Lewinsohn, E., Department of Vegetable Crops, Agricultural Research Organization, Newe Ya'ar Res. Ctr., P.O. B., Israel
Facilitators :
From page:
1257
To page:
1262
(
Total pages:
6
)
Abstract:
Koubo [Cereus peruvianus (L.) Miller, Cactaceae] is a commercially grown columnar cactus that produces an apple-sized, berry-like, edible fruit. The unique aroma of this fruit is largely due to (S)-linalool and linalool derivatives. Cell-free extracts obtained from koubo fruits produced linalool from geranyl diphosphate (GDP) (apparent K m 18 μM) in enzymatic assays containing the divalent ion cofactor Mn 2+ or Mg 2+. The predominant enantiomer produced in vitro was (S)-linalool, with an optical purity of 85%. Incubation of geranyl diphosphate in the presence of 1-10 mM FeSO 4 or FeCl 2, without added enzyme, supported the in vitro spontaneous formation of racemic linalool. The koubo linalool synthase displayed a molecular mass of 53 kDa as determined by gel permeation chromatography. Enzyme activity levels were negligible in green immature fruits and increased with fruit development and during storage, concomitant with the timing of linalool accumulation in fruits. © 2004 Elsevier Ireland Ltd. All rights reserved.
Note:
Related Files :
biosynthesis
Cereus hildmannianus
enzymes
Fruits
Molecular Biology
Plants
ripening
עוד תגיות
תוכן קשור
More details
DOI :
10.1016/j.plantsci.2004.06.024
Article number:
0
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
27371
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:30
Scientific Publication
S-Linalool synthase activity in developing fruit of the columnar cactus koubo [Cereus peruvianus (L.) Miller]
167
Sitrit, Y., Institutes for Applied Research, Ben-Gurion University of the Negev, P.O. Box 653, Beer-Sheva 84105, I., Israel
Ninio, R., Institutes for Applied Research, Ben-Gurion University of the Negev, P.O. Box 653, Beer-Sheva 84105, I., Israel, Department of Life Sciences, Ben-Gurion University of the Negev, P.O. Box 653, Beer-Sheva 84105, I., Israel
Bar, E., Department of Vegetable Crops, Agricultural Research Organization, Newe Ya'ar Res. Ctr., P.O. B., Israel
Golan, E., Department of Vegetable Crops, Agricultural Research Organization, Newe Ya'ar Res. Ctr., P.O. B., Israel
Larkov, O., Department of Vegetable Crops, Agricultural Research Organization, Newe Ya'ar Res. Ctr., P.O. B., Israel
Ravid, U., Department of Vegetable Crops, Agricultural Research Organization, Newe Ya'ar Res. Ctr., P.O. B., Israel
Lewinsohn, E., Department of Vegetable Crops, Agricultural Research Organization, Newe Ya'ar Res. Ctr., P.O. B., Israel
S-Linalool synthase activity in developing fruit of the columnar cactus koubo [Cereus peruvianus (L.) Miller]
Koubo [Cereus peruvianus (L.) Miller, Cactaceae] is a commercially grown columnar cactus that produces an apple-sized, berry-like, edible fruit. The unique aroma of this fruit is largely due to (S)-linalool and linalool derivatives. Cell-free extracts obtained from koubo fruits produced linalool from geranyl diphosphate (GDP) (apparent K m 18 μM) in enzymatic assays containing the divalent ion cofactor Mn 2+ or Mg 2+. The predominant enantiomer produced in vitro was (S)-linalool, with an optical purity of 85%. Incubation of geranyl diphosphate in the presence of 1-10 mM FeSO 4 or FeCl 2, without added enzyme, supported the in vitro spontaneous formation of racemic linalool. The koubo linalool synthase displayed a molecular mass of 53 kDa as determined by gel permeation chromatography. Enzyme activity levels were negligible in green immature fruits and increased with fruit development and during storage, concomitant with the timing of linalool accumulation in fruits. © 2004 Elsevier Ireland Ltd. All rights reserved.
Scientific Publication
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