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אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
Growth hormone and insulin-like growth factor I regulate collagen gene expression and extracellular collagen in cultures of avian skin fibroblasts
Year:
1991
Authors :
גרנות, עירית
;
.
הורביץ, שמואל
;
.
פינס, מרק
;
.
Volume :
80
Co-Authors:
Granot, I., Institute of Animal Science, Agricultural Research Organization, The Volcani Center, Bet Dagan, 50250, Israel
Halevy, O., Department of Animal Science, Faculty of Agriculture, The Hebrew University, Rehovot, 76100, Israel
Hurwitz, S., Institute of Animal Science, Agricultural Research Organization, The Volcani Center, Bet Dagan, 50250, Israel
Pines, M., Institute of Animal Science, Agricultural Research Organization, The Volcani Center, Bet Dagan, 50250, Israel
Facilitators :
From page:
1
To page:
9
(
Total pages:
9
)
Abstract:
Avian skin fibroblasts were isolated, cultured and incubated with [3H]proline for 24 h. The cells exported radiolabeled collagenase-digestible (CDP) and non-collagenase-digestible (NCDP) proteins into the medium. Human, bovine and avian growth hormone (GH) as well as insulin-like growth factor I (IGF-I) attenuated the appearance of [3H]CDP in the medium without affecting [3H]NCDP. The appearance of [3H]CDP was not affected by prolactin. The effects of GH and IGF-I were enhanced by increasing concentrations of fetal calf serum (FCS). A synergism was observed between GH and IGF-I in their effect on CDP. Each peptide, at an ineffective concentration, increased the sensitivity of the cells to the other peptide. Collagenase activity in the medium was enhanced by IGF-I, but not modified by GH, FCS, or by their interaction with IGF-I. GH and IGF-I inhibition of type I procollagen gene expression was demonstrated with the aid of probes containing sequences corresponding to the mRNAs for avian αI and αII chains. The results suggest that GH and IGF-I cooperate in regulating collagen synthesis, but collagen degradation is affected by IGF-I and not by GH. © 1991.
Note:
Related Files :
Animal
animal cell
cattle
Chickens
gene expression regulation
human
Insulin-Like Growth Factor I
skin fibroblast
עוד תגיות
תוכן קשור
More details
DOI :
10.1016/0303-7207(91)90137-H
Article number:
0
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
27415
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:30
You may also be interested in
Scientific Publication
Growth hormone and insulin-like growth factor I regulate collagen gene expression and extracellular collagen in cultures of avian skin fibroblasts
80
Granot, I., Institute of Animal Science, Agricultural Research Organization, The Volcani Center, Bet Dagan, 50250, Israel
Halevy, O., Department of Animal Science, Faculty of Agriculture, The Hebrew University, Rehovot, 76100, Israel
Hurwitz, S., Institute of Animal Science, Agricultural Research Organization, The Volcani Center, Bet Dagan, 50250, Israel
Pines, M., Institute of Animal Science, Agricultural Research Organization, The Volcani Center, Bet Dagan, 50250, Israel
Growth hormone and insulin-like growth factor I regulate collagen gene expression and extracellular collagen in cultures of avian skin fibroblasts
Avian skin fibroblasts were isolated, cultured and incubated with [3H]proline for 24 h. The cells exported radiolabeled collagenase-digestible (CDP) and non-collagenase-digestible (NCDP) proteins into the medium. Human, bovine and avian growth hormone (GH) as well as insulin-like growth factor I (IGF-I) attenuated the appearance of [3H]CDP in the medium without affecting [3H]NCDP. The appearance of [3H]CDP was not affected by prolactin. The effects of GH and IGF-I were enhanced by increasing concentrations of fetal calf serum (FCS). A synergism was observed between GH and IGF-I in their effect on CDP. Each peptide, at an ineffective concentration, increased the sensitivity of the cells to the other peptide. Collagenase activity in the medium was enhanced by IGF-I, but not modified by GH, FCS, or by their interaction with IGF-I. GH and IGF-I inhibition of type I procollagen gene expression was demonstrated with the aid of probes containing sequences corresponding to the mRNAs for avian αI and αII chains. The results suggest that GH and IGF-I cooperate in regulating collagen synthesis, but collagen degradation is affected by IGF-I and not by GH. © 1991.
Scientific Publication
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