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אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
Genetic diversity within Colletotrichum acutatum sensu simmonds
Year:
2001
Source of publication :
Phytopathology
Authors :
מימון, מרסל
;
.
מינץ, דרור
;
.
פרימן, סטנלי
;
.
צוויבל, אאידה
;
.
Volume :
91
Co-Authors:
Freeman, S., Department of Plant Pathology, ARO, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Minz, D., Department of Plant Pathology, ARO, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Maymon, M., Department of Plant Pathology, ARO, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Zveibil, A., Department of Plant Pathology, ARO, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Facilitators :
From page:
586
To page:
592
(
Total pages:
7
)
Abstract:
Isolates of Colletotrichum acutatum from several hosts were characterized by various molecular methods in comparison with morphological identification. Species-specific primer analysis was reliable for grouping C. acutatum isolates to their designated species. Arbitrarily primed polymerase chain reaction and A+T-rich DNA analyses identified four subgroups within C. acutatum. Subgroup I contained U.S. isolates from almond, apple, peach, and pecan, subgroup II contained isolates from anemone, olive, and strawberry, subgroup III contained isolates from almond (Israel) and strawberry (Spain), and subgroup IV contained a single isolate from anemone (the Netherlands). Likewise, sequence analysis of the internal transcribed spacer (ITS) 2 region alone or the complete ITS (ITS 1-5.8S-ITS 2) region grouped the isolates into the same four subgroups. Percent similarity of the complete ITS region within each cluster ranged from 99.6 to 100.0, 99.8 to 100.0, and 98.6% among subgroups I, II, and III, respectively. DNA sequence analysis of the ITS 2 region alone or the entire ITS 1-2 region was more informative than that of the ITS 1 region, which could only group the isolates into two main clusters. The molecular methods employed for studying genetic variation in populations of C. acutatum determined that this species is diverse, indicating that isolates within populations of each subgroup are not host specific.
Note:
Related Files :
Cluster analysis
host specificity
Israel
Polymerase Chain Reaction
sequence analysis
Spain
עוד תגיות
תוכן קשור
More details
DOI :
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
27423
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:30
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Scientific Publication
Genetic diversity within Colletotrichum acutatum sensu simmonds
91
Freeman, S., Department of Plant Pathology, ARO, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Minz, D., Department of Plant Pathology, ARO, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Maymon, M., Department of Plant Pathology, ARO, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Zveibil, A., Department of Plant Pathology, ARO, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Genetic diversity within Colletotrichum acutatum sensu simmonds
Isolates of Colletotrichum acutatum from several hosts were characterized by various molecular methods in comparison with morphological identification. Species-specific primer analysis was reliable for grouping C. acutatum isolates to their designated species. Arbitrarily primed polymerase chain reaction and A+T-rich DNA analyses identified four subgroups within C. acutatum. Subgroup I contained U.S. isolates from almond, apple, peach, and pecan, subgroup II contained isolates from anemone, olive, and strawberry, subgroup III contained isolates from almond (Israel) and strawberry (Spain), and subgroup IV contained a single isolate from anemone (the Netherlands). Likewise, sequence analysis of the internal transcribed spacer (ITS) 2 region alone or the complete ITS (ITS 1-5.8S-ITS 2) region grouped the isolates into the same four subgroups. Percent similarity of the complete ITS region within each cluster ranged from 99.6 to 100.0, 99.8 to 100.0, and 98.6% among subgroups I, II, and III, respectively. DNA sequence analysis of the ITS 2 region alone or the entire ITS 1-2 region was more informative than that of the ITS 1 region, which could only group the isolates into two main clusters. The molecular methods employed for studying genetic variation in populations of C. acutatum determined that this species is diverse, indicating that isolates within populations of each subgroup are not host specific.
Scientific Publication
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