Co-Authors:
Hanania, U., Department of Fruit Tree Sciences, Institute of Plant Sciences, Agricultural Research Organization, P.O. Box 6, 50250 Bet Dagan, Israel
Velcheva, M., Department of Fruit Tree Sciences, Institute of Plant Sciences, Agricultural Research Organization, P.O. Box 6, 50250 Bet Dagan, Israel, Alberta Research Council, P.O. Bag 4000, Hwy 16A and 75 Street, Vegreville, AB T9C 1T4, Canada
Sahar, N., Department of Fruit Tree Sciences, Institute of Plant Sciences, Agricultural Research Organization, P.O. Box 6, 50250 Bet Dagan, Israel
Flaishman, M., Department of Fruit Tree Sciences, Institute of Plant Sciences, Agricultural Research Organization, P.O. Box 6, 50250 Bet Dagan, Israel
Or, E., Department of Fruit Tree Sciences, Institute of Plant Sciences, Agricultural Research Organization, P.O. Box 6, 50250 Bet Dagan, Israel
Degani, O., Department of Fruit Tree Sciences, Institute of Plant Sciences, Agricultural Research Organization, P.O. Box 6, 50250 Bet Dagan, Israel
Perl, A., Department of Fruit Tree Sciences, Institute of Plant Sciences, Agricultural Research Organization, P.O. Box 6, 50250 Bet Dagan, Israel
Abstract:
In Vitis vinifera L. cv. Thompson Seedless, fertilization occurs but seeds abort, a type of stenospermocarpy. To clone transcripts with differential expression during flower development, suppressive subtractive hybridization was carried out using two isogenic clones 'Thompson seedless' and 'Thompson seeded', at three stages of inflorescence development (from bud break to ~20 days prior to anthesis). Differential screening and sequencing of a forward and reverse subtractive cDNA library yielded several singleton ESTs. One differentially expressed clone in 'Thompson' seeded versus seedless isogenic clones was the ubiquitin extension protein S27a. In situ hybridization demonstrated its significantly higher expression in the carpel and ovaries of 'Thompson' seedless versus seeded isogenic clones during flower development. Overexpression of this gene resulted in abnormal plant regeneration and inhibited shoot development compared to controls; its silencing in embryogenic callus induced cell necrosis and callus death, evidencing tight regulation of this gene in developing organs of grape. S27a overexpression in carpels and integuments of the seedless flower may interfere with normal development of these organs, leading to embryo abortion and seedlessness. © 2009 Springer-Verlag.
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