אסיף מאגר המחקר החקלאי

Note: Identification of Trichoderma biocontrol isolates to clades according to ap-PCR and ITS sequence analyses

Year:

2004

Source of publication :

Authors :

Volume :

32

Co-Authors:

Maymon, M., Dept. of Plant Pathology, ARO, Volcani Center, Bet Dagan 50250, Israel
Minz, D., Inst. Soil, Water and Environ. Sci., ARO, Volcani Center, Bet Dagan 50250, Israel
Barbul, O., Dept. of Plant Pathology, ARO, Volcani Center, Bet Dagan 50250, Israel
Zveibil, A., Dept. of Plant Pathology, ARO, Volcani Center, Bet Dagan 50250, Israel
Elad, Y., Dept. of Plant Pathology, ARO, Volcani Center, Bet Dagan 50250, Israel
Freeman, S., Dept. of Plant Pathology, ARO, Volcani Center, Bet Dagan 50250, Israel

Facilitators :

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Abstract:

A collection of Trichoderma isolates, with different biocontrol capabilities, were identified by molecular methods. Arbitrarily-primed PCR (ap-PCR) using repeat motif primers was performed on DNA from a Trichoderma spp. collection of 76 isolates, and representative isolates were further characterized into three main clades by internal transcribed spacer (ITS) sequence analysis. Consequently, a reliable phylogenetic tree was constructed containing isolates belonging to the T. harzianum clade (comprising T. aureoviride, T. inhamatum, and T. virens), the T. longibrachiatum and T. saturnisporum cluster, and that including the species T. asperellum, T. atroviride, T. koningii and T. viride.

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