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פותח על ידי קלירמאש פתרונות בע"מ -
Incorporation of 5-bromodeoxyuridine into DNA of wild type Escherichia coli and its use for the enrichment of auxotrophic mutants
Year:
1970
Source of publication :
MGG Molecular & General Genetics
Authors :
רוזנר, אריה
;
.
Volume :
106
Co-Authors:
Rosner, A., Department of Biochemistry, Tel-Aviv University, Tel-Aviv, Israel
Yagil, E., Department of Biochemistry, Tel-Aviv University, Tel-Aviv, Israel
Facilitators :
From page:
254
To page:
262
(
Total pages:
9
)
Abstract:
The effect of deoxyadenosine (AdR) and 5-fluorodeoxyuridine (FUdR) on the incorporation of 5-bromodeoxyuridine (BUdR) into DNA of thymine-non requiring cells of Escherichia coli was studied. This incorporation renders the cells sensitive to irradiation at near-UV light. It was found that the combination of AdR and FUdR increases incorporation of BUdR and sensitivity to irradiation. An enrichment of auxotrophs by a factor of 105 was obtained from a mixed population grown in minimal medium containing BUdR, AdR and FUdR and irradiated subsequently. © 1970 Springer-Verlag.
Note:
Related Files :
metabolism
Methods
mutation
nucleoside
tritium
ultraviolet radiation
Ultraviolet Rays
עוד תגיות
תוכן קשור
More details
DOI :
10.1007/BF00340384
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
27620
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:32
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Scientific Publication
Incorporation of 5-bromodeoxyuridine into DNA of wild type Escherichia coli and its use for the enrichment of auxotrophic mutants
106
Rosner, A., Department of Biochemistry, Tel-Aviv University, Tel-Aviv, Israel
Yagil, E., Department of Biochemistry, Tel-Aviv University, Tel-Aviv, Israel
Incorporation of 5-bromodeoxyuridine into DNA of wild type Escherichia coli and its use for the enrichment of auxotrophic mutants
The effect of deoxyadenosine (AdR) and 5-fluorodeoxyuridine (FUdR) on the incorporation of 5-bromodeoxyuridine (BUdR) into DNA of thymine-non requiring cells of Escherichia coli was studied. This incorporation renders the cells sensitive to irradiation at near-UV light. It was found that the combination of AdR and FUdR increases incorporation of BUdR and sensitivity to irradiation. An enrichment of auxotrophs by a factor of 105 was obtained from a mixed population grown in minimal medium containing BUdR, AdR and FUdR and irradiated subsequently. © 1970 Springer-Verlag.
Scientific Publication
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