חיפוש מתקדם
MGG Molecular & General Genetics
Rosner, A., Department of Biochemistry, Tel-Aviv University, Tel-Aviv, Israel
Yagil, E., Department of Biochemistry, Tel-Aviv University, Tel-Aviv, Israel
The effect of deoxyadenosine (AdR) and 5-fluorodeoxyuridine (FUdR) on the incorporation of 5-bromodeoxyuridine (BUdR) into DNA of thymine-non requiring cells of Escherichia coli was studied. This incorporation renders the cells sensitive to irradiation at near-UV light. It was found that the combination of AdR and FUdR increases incorporation of BUdR and sensitivity to irradiation. An enrichment of auxotrophs by a factor of 105 was obtained from a mixed population grown in minimal medium containing BUdR, AdR and FUdR and irradiated subsequently. © 1970 Springer-Verlag.
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הספר "אוצר וולקני"
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תנאי שימוש
Incorporation of 5-bromodeoxyuridine into DNA of wild type Escherichia coli and its use for the enrichment of auxotrophic mutants
106
Rosner, A., Department of Biochemistry, Tel-Aviv University, Tel-Aviv, Israel
Yagil, E., Department of Biochemistry, Tel-Aviv University, Tel-Aviv, Israel
Incorporation of 5-bromodeoxyuridine into DNA of wild type Escherichia coli and its use for the enrichment of auxotrophic mutants
The effect of deoxyadenosine (AdR) and 5-fluorodeoxyuridine (FUdR) on the incorporation of 5-bromodeoxyuridine (BUdR) into DNA of thymine-non requiring cells of Escherichia coli was studied. This incorporation renders the cells sensitive to irradiation at near-UV light. It was found that the combination of AdR and FUdR increases incorporation of BUdR and sensitivity to irradiation. An enrichment of auxotrophs by a factor of 105 was obtained from a mixed population grown in minimal medium containing BUdR, AdR and FUdR and irradiated subsequently. © 1970 Springer-Verlag.
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