חיפוש מתקדם
תחביר
חפש...
הספר "אוצר וולקני"
אודות
תנאי שימוש
ניהול
קהילה:
אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
Expression in transgenic mice of two genes of different tissue specificity integrated into a single chromosomal site.
Year:
1987
Source of publication :
Genes & development
Authors :
שני, משה
;
.
Volume :
1
Co-Authors:
Einat, P., Department of Cell Biology, Weizmann Institute of Science, Rehovot, Israel.
Bergman, Y., Department of Cell Biology, Weizmann Institute of Science, Rehovot, Israel.
Yaffe, D., Department of Cell Biology, Weizmann Institute of Science, Rehovot, Israel.
Shani, M., Department of Cell Biology, Weizmann Institute of Science, Rehovot, Israel.
Facilitators :
From page:
1075
To page:
1084
(
Total pages:
10
)
Abstract:
Transgenic mice were used to study the expression of pairs of genes with distinctly different tissue specificities, covalently linked and integrated into the same chromosomal site. A transgenic strain carrying, in close proximity and in the same orientation, the rat fast skeletal muscle myosin light-chain 2 (MLC2) gene and the mouse rearranged immunoglobulin kappa light-chain gene expressed the immunoglobulin gene specifically in the lymphoid tissues, whereas rat MLC2 transcripts were found in skeletal muscle but not in the spleen or the other tissues that were tested. In another transgenic strain, carrying the rat MLC2 gene and a modified rat skeletal muscle actin gene (actin-globin chimeric gene), transcripts of the rat MLC2 gene were detected in skeletal muscle only, whereas the actin-globin transcripts were detected in skeletal muscle as well as in the heart. Moreover, the expression of the chimeric gene was also developmentally regulated. Expression was higher in cardiac muscle than in the skeletal muscle of neonatal mice, whereas expression was higher in skeletal muscle in adult mice. This pattern is consistent with the regulation of the expression of the endogenous skeletal muscle actin gene. Thus, in those transgenic strains that expressed both genes, each gene retained its tissue specificity, in spite of their close proximity. These results indicate a high degree of autonomy of the control elements included in the cloned genomic DNA fragment and demonstrate that a single chromosomal site can be permissive for the proper expression of two genes with different tissue specificities.
Note:
Related Files :
Animal
gene expression regulation
genetic linkage
Genetics
Globins
immunoglobulin kappa chain
mice
myosin
spleen
עוד תגיות
תוכן קשור
More details
DOI :
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
27631
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:32
You may also be interested in
Scientific Publication
Expression in transgenic mice of two genes of different tissue specificity integrated into a single chromosomal site.
1
Einat, P., Department of Cell Biology, Weizmann Institute of Science, Rehovot, Israel.
Bergman, Y., Department of Cell Biology, Weizmann Institute of Science, Rehovot, Israel.
Yaffe, D., Department of Cell Biology, Weizmann Institute of Science, Rehovot, Israel.
Shani, M., Department of Cell Biology, Weizmann Institute of Science, Rehovot, Israel.
Expression in transgenic mice of two genes of different tissue specificity integrated into a single chromosomal site.
Transgenic mice were used to study the expression of pairs of genes with distinctly different tissue specificities, covalently linked and integrated into the same chromosomal site. A transgenic strain carrying, in close proximity and in the same orientation, the rat fast skeletal muscle myosin light-chain 2 (MLC2) gene and the mouse rearranged immunoglobulin kappa light-chain gene expressed the immunoglobulin gene specifically in the lymphoid tissues, whereas rat MLC2 transcripts were found in skeletal muscle but not in the spleen or the other tissues that were tested. In another transgenic strain, carrying the rat MLC2 gene and a modified rat skeletal muscle actin gene (actin-globin chimeric gene), transcripts of the rat MLC2 gene were detected in skeletal muscle only, whereas the actin-globin transcripts were detected in skeletal muscle as well as in the heart. Moreover, the expression of the chimeric gene was also developmentally regulated. Expression was higher in cardiac muscle than in the skeletal muscle of neonatal mice, whereas expression was higher in skeletal muscle in adult mice. This pattern is consistent with the regulation of the expression of the endogenous skeletal muscle actin gene. Thus, in those transgenic strains that expressed both genes, each gene retained its tissue specificity, in spite of their close proximity. These results indicate a high degree of autonomy of the control elements included in the cloned genomic DNA fragment and demonstrate that a single chromosomal site can be permissive for the proper expression of two genes with different tissue specificities.
Scientific Publication
נגישות
menu      
You may also be interested in