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פותח על ידי קלירמאש פתרונות בע"מ -
A selective medium for improving quantitative isolation of Trichoderma spp. from soil
Year:
1981
Source of publication :
Phytoparasitica
Authors :
אלעד, יגאל
;
.
Volume :
9
Co-Authors:
Elad, Y., Dept. of Plant Pathology and Microbiology, The Hebrew University of Jerusalem, Faculty of Agriculture, Rehovot, Israel
Chet, I., Dept. of Plant Pathology and Microbiology, The Hebrew University of Jerusalem, Faculty of Agriculture, Rehovot, Israel
Henis, Y., Dept. of Plant Pathology and Microbiology, The Hebrew University of Jerusalem, Faculty of Agriculture, Rehovot, Israel
Facilitators :
From page:
59
To page:
67
(
Total pages:
9
)
Abstract:
A Trichoderma-selective agar medium (TSM) was developed for quantitative isolation of Trichoderma spp. from soil. Selectivity was obtained by using chloramphenicol as a bacterial inhibitor, and pentachloronitrobenzene, p-dimethylaminobenzenediazo sodium sulfonate and rose-bengal as selective fungal inhibitors. The TSM also contains a low concentration of glucose which still allows relatively rapid growth and sporulation of Trichoderma, enabling convenient and rapid identification of Trichoderma colonies. All the 15 Trichoderma isolates tested formed colonies and grew well on this medium. Recovery of Trichoderma from artificially inoculated soils was high and was not affected by soil type or by other microorganisms. A positive correlation was observed between Trichoderma added to soil and counts of Trichoderma colonies on TSM plates. When combined with a soil pellet sampler, the selective medium was also used successfully for recovery of the indigenous Trichoderma population of natural soils. © 1981 Springer Science + Business Media B.V.
Note:
Related Files :
BioControl
Trichoderma harzianum
עוד תגיות
תוכן קשור
More details
DOI :
10.1007/BF03158330
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
27641
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:32
Scientific Publication
A selective medium for improving quantitative isolation of Trichoderma spp. from soil
9
Elad, Y., Dept. of Plant Pathology and Microbiology, The Hebrew University of Jerusalem, Faculty of Agriculture, Rehovot, Israel
Chet, I., Dept. of Plant Pathology and Microbiology, The Hebrew University of Jerusalem, Faculty of Agriculture, Rehovot, Israel
Henis, Y., Dept. of Plant Pathology and Microbiology, The Hebrew University of Jerusalem, Faculty of Agriculture, Rehovot, Israel
A selective medium for improving quantitative isolation of Trichoderma spp. from soil
A Trichoderma-selective agar medium (TSM) was developed for quantitative isolation of Trichoderma spp. from soil. Selectivity was obtained by using chloramphenicol as a bacterial inhibitor, and pentachloronitrobenzene, p-dimethylaminobenzenediazo sodium sulfonate and rose-bengal as selective fungal inhibitors. The TSM also contains a low concentration of glucose which still allows relatively rapid growth and sporulation of Trichoderma, enabling convenient and rapid identification of Trichoderma colonies. All the 15 Trichoderma isolates tested formed colonies and grew well on this medium. Recovery of Trichoderma from artificially inoculated soils was high and was not affected by soil type or by other microorganisms. A positive correlation was observed between Trichoderma added to soil and counts of Trichoderma colonies on TSM plates. When combined with a soil pellet sampler, the selective medium was also used successfully for recovery of the indigenous Trichoderma population of natural soils. © 1981 Springer Science + Business Media B.V.
Scientific Publication
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