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פותח על ידי קלירמאש פתרונות בע"מ -
Diagnosis of broad bean wilt virus 1 and verbena latent virus in tropaeolum majus in New Zealand
Year:
2010
Source of publication :
Australasian Plant Pathology
Authors :
גרה, עבדאללה
;
.
Volume :
39
Co-Authors:
Ochoa-Corona, F.M., Plant Health and Environment Laboratory, MAF Biosecurity New Zealand, PO Box 2095, Auckland 1140, New Zealand, Oklahoma State University, Department of Entomology and Plant Pathology, NIMFFAB, 127 NRC, Stillwater, OK 74078, United States
Tang, J., Plant Health and Environment Laboratory, MAF Biosecurity New Zealand, PO Box 2095, Auckland 1140, New Zealand
Lebas, B.S.M., Plant Health and Environment Laboratory, MAF Biosecurity New Zealand, PO Box 2095, Auckland 1140, New Zealand
Rubio, L., Instituto Valenciano de Investigaciones Agrarias (IVIA), Apartado Official, 46113 Moncada (Valencia), Spain
Gera, A., Institute of Plant Protection, Agricultural Research Organisation, Volcani Centre, PO Box 6, Bet Dagan 50250, Israel
Alexander, B.J.R., Plant Health and Environment Laboratory, MAF Biosecurity New Zealand, PO Box 2095, Auckland 1140, New Zealand
Facilitators :
From page:
120
To page:
124
(
Total pages:
5
)
Abstract:
A Tropaeolum majus (nasturtium) plant with severe leaf mosaic symptoms was collected from a public park in New Zealand in September 2004. Electron microscopy of crude sap preparations revealed the presence of flexuous filamentous virus particles of different sizes. Necrotic local lesions followed by systemic leaf necrosis, chlorosis, mosaic or distortion developed on nine species of herbaceous indicator plants. The original sample and indicator plants tested positive for Broad bean wilt virus 1 (BBWV-1, genus Fabavirus) and Verbena latent virus (VeLV, genus Carlavirus) by enzyme-linked immunosorbent assay (ELISA). BBWV-1 was confirmed by reverse transcriptionpolymerase chain reaction (RT-PCR) using generic fabavirus primers and specific BBWV-1 primers. A RT-PCR using specific primers to VeLV was developed. The BBWV-1 and VeLV amplicons each showed 96% nucleotide identity to published sequences of BBWV-1 isolate PV-132 (New York, USA) and VeLV from Israel, respectively. The sample was also coinfected with Turnip mosaic virus (genus Potyvirus) and Opium poppy mosaic virus (unassigned member of the genus Umbravirus). This is the first report of BBWV-1 in New Zealand and the first report of VeLV infecting T. majus worldwide. T. majus is a common weed in New Zealand and may represent a significant reservoir for these viruses. © 2010 Australasian Plant Pathology Society.
Note:
Related Files :
Brassica rapa subsp. rapa
Broad bean wilt virus 1
Tropaeolaceae
Tropaeolum majus
Turnip mosaic virus
Verbena latent virus
Vicia faba
עוד תגיות
תוכן קשור
More details
DOI :
10.1071/AP09070
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
27667
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:32
Scientific Publication
Diagnosis of broad bean wilt virus 1 and verbena latent virus in tropaeolum majus in New Zealand
39
Ochoa-Corona, F.M., Plant Health and Environment Laboratory, MAF Biosecurity New Zealand, PO Box 2095, Auckland 1140, New Zealand, Oklahoma State University, Department of Entomology and Plant Pathology, NIMFFAB, 127 NRC, Stillwater, OK 74078, United States
Tang, J., Plant Health and Environment Laboratory, MAF Biosecurity New Zealand, PO Box 2095, Auckland 1140, New Zealand
Lebas, B.S.M., Plant Health and Environment Laboratory, MAF Biosecurity New Zealand, PO Box 2095, Auckland 1140, New Zealand
Rubio, L., Instituto Valenciano de Investigaciones Agrarias (IVIA), Apartado Official, 46113 Moncada (Valencia), Spain
Gera, A., Institute of Plant Protection, Agricultural Research Organisation, Volcani Centre, PO Box 6, Bet Dagan 50250, Israel
Alexander, B.J.R., Plant Health and Environment Laboratory, MAF Biosecurity New Zealand, PO Box 2095, Auckland 1140, New Zealand
Diagnosis of broad bean wilt virus 1 and verbena latent virus in tropaeolum majus in New Zealand
A Tropaeolum majus (nasturtium) plant with severe leaf mosaic symptoms was collected from a public park in New Zealand in September 2004. Electron microscopy of crude sap preparations revealed the presence of flexuous filamentous virus particles of different sizes. Necrotic local lesions followed by systemic leaf necrosis, chlorosis, mosaic or distortion developed on nine species of herbaceous indicator plants. The original sample and indicator plants tested positive for Broad bean wilt virus 1 (BBWV-1, genus Fabavirus) and Verbena latent virus (VeLV, genus Carlavirus) by enzyme-linked immunosorbent assay (ELISA). BBWV-1 was confirmed by reverse transcriptionpolymerase chain reaction (RT-PCR) using generic fabavirus primers and specific BBWV-1 primers. A RT-PCR using specific primers to VeLV was developed. The BBWV-1 and VeLV amplicons each showed 96% nucleotide identity to published sequences of BBWV-1 isolate PV-132 (New York, USA) and VeLV from Israel, respectively. The sample was also coinfected with Turnip mosaic virus (genus Potyvirus) and Opium poppy mosaic virus (unassigned member of the genus Umbravirus). This is the first report of BBWV-1 in New Zealand and the first report of VeLV infecting T. majus worldwide. T. majus is a common weed in New Zealand and may represent a significant reservoir for these viruses. © 2010 Australasian Plant Pathology Society.
Scientific Publication
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