חיפוש מתקדם
Virology

Hak, H., Institute of Plant Sciences, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel, Department of Biological Chemistry, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Israel
Levy, Y., Institute of Plant Sciences, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel
Chandran, S.A., Institute of Plant Sciences, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel
Belausov, E., Institute of Plant Sciences, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel
Loyter, A., Department of Biological Chemistry, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Israel
Lapidot, M., Institute of Plant Sciences, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel
Gafni, Y., Institute of Plant Sciences, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel

Tomato yellow leaf curl virus (TYLCV), a major tomato pathogen causing extensive crop losses, is a whitefly-transmitted geminivirus. V2 mutants of TYLCV-Is and related viruses tend to induce symptomless infection with attenuated viral DNA levels, while accumulating close to wild-type DNA levels in protoplasts, suggesting V2 as a movement protein. The discovery of plant-silencing mechanisms and viral silencing suppressors, V2 included, led us to reconsider V2's involvement in viral movement. We studied two mutant versions of the virus, one impaired in V2 silencing-suppression activity, and another carrying a non-translatable V2. While both mutant viruses spread in the infected plant to newly emerged leaves at the same rate as the wild-type virus, their DNA-accumulation levels were tenfold lower than in the wild-type virus. Thus, we suggest that the setback in virus proliferation, previously ascribed to a movement impediment, is due to lack of silencing-suppression activity. © 2015 Elsevier Inc.
פותח על ידי קלירמאש פתרונות בע"מ -
הספר "אוצר וולקני"
אודות
תנאי שימוש
TYLCV-Is movement in planta does not require V2 protein
477

Hak, H., Institute of Plant Sciences, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel, Department of Biological Chemistry, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Israel
Levy, Y., Institute of Plant Sciences, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel
Chandran, S.A., Institute of Plant Sciences, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel
Belausov, E., Institute of Plant Sciences, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel
Loyter, A., Department of Biological Chemistry, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Israel
Lapidot, M., Institute of Plant Sciences, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel
Gafni, Y., Institute of Plant Sciences, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel

TYLCV-Is movement in planta does not require V2 protein
Tomato yellow leaf curl virus (TYLCV), a major tomato pathogen causing extensive crop losses, is a whitefly-transmitted geminivirus. V2 mutants of TYLCV-Is and related viruses tend to induce symptomless infection with attenuated viral DNA levels, while accumulating close to wild-type DNA levels in protoplasts, suggesting V2 as a movement protein. The discovery of plant-silencing mechanisms and viral silencing suppressors, V2 included, led us to reconsider V2's involvement in viral movement. We studied two mutant versions of the virus, one impaired in V2 silencing-suppression activity, and another carrying a non-translatable V2. While both mutant viruses spread in the infected plant to newly emerged leaves at the same rate as the wild-type virus, their DNA-accumulation levels were tenfold lower than in the wild-type virus. Thus, we suggest that the setback in virus proliferation, previously ascribed to a movement impediment, is due to lack of silencing-suppression activity. © 2015 Elsevier Inc.
Scientific Publication
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