נגישות
menu      
חיפוש מתקדם
תחביר
חפש...
הספר "אוצר וולקני"
אודות
תנאי שימוש
ניהול
קהילה:
אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
Antigenic cross-reactivity among monoterpene cyclases from grand fir and induction of these enzymes upon stem wounding
Year:
1992
Authors :
לוינסון, אפרים
;
.
Volume :
294
Co-Authors:
Gijzen, M., Institute of Biological Chemistry, Department of Biochemistry and Biophysics, Washington State University, Pullman, WA 99164-6340, United States
Lewinsohn, E., Institute of Biological Chemistry, Department of Biochemistry and Biophysics, Washington State University, Pullman, WA 99164-6340, United States
Croteau, R., Institute of Biological Chemistry, Department of Biochemistry and Biophysics, Washington State University, Pullman, WA 99164-6340, United States
Facilitators :
From page:
670
To page:
674
(
Total pages:
5
)
Abstract:
A major wound response in grand fir (Abies grandis) sapling stems is the rapid increase in monoterpene production at the site of injury. Monoterpene cyclases (synthases) catalyze the formation of monoterpenes from geranyl pyrophosphate, and total cyclase activity increases markedly on wounding. At least six distinct cyclases, producing different monoterpene products, have been isolated from wounded grand fir saplings and characterized. The predominant wound-inducible cyclase produces both α- and β-pinene. This pinene cyclase was purified, and polyclonal antibodies were generated in rabbits against the sodium dodecyl sulfate-denatured protein. The antibody preparation was found to crossreact by Western blotting with other grand fir monoterpene cyclases that produce different olefinic products, but not with monoterpene cyclases from related conifer species (Pinus contorta and P. ponderosa) or from angiosperms (Mentha piperita and M. spicata). The increase in monoterpene cyclase activity after wounding was closely correlated with the appearance of new cyclase protein as determined by immunoblotting. These results indicate that the wound-dependent increase in monoterpene cyclase activity is a consequence of de novo synthesis of cyclase protein. © 1992.
Note:
Related Files :
Cross Reactions
Electrophoresis, Polyacrylamide Gel
enzyme activity
Mentha spicata
Mentha x piperita
Plants
terpene
trees
עוד תגיות
תוכן קשור
More details
DOI :
10.1016/0003-9861(92)90740-N
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
27714
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:33
You may also be interested in
Scientific Publication
Antigenic cross-reactivity among monoterpene cyclases from grand fir and induction of these enzymes upon stem wounding
294
Gijzen, M., Institute of Biological Chemistry, Department of Biochemistry and Biophysics, Washington State University, Pullman, WA 99164-6340, United States
Lewinsohn, E., Institute of Biological Chemistry, Department of Biochemistry and Biophysics, Washington State University, Pullman, WA 99164-6340, United States
Croteau, R., Institute of Biological Chemistry, Department of Biochemistry and Biophysics, Washington State University, Pullman, WA 99164-6340, United States
Antigenic cross-reactivity among monoterpene cyclases from grand fir and induction of these enzymes upon stem wounding
A major wound response in grand fir (Abies grandis) sapling stems is the rapid increase in monoterpene production at the site of injury. Monoterpene cyclases (synthases) catalyze the formation of monoterpenes from geranyl pyrophosphate, and total cyclase activity increases markedly on wounding. At least six distinct cyclases, producing different monoterpene products, have been isolated from wounded grand fir saplings and characterized. The predominant wound-inducible cyclase produces both α- and β-pinene. This pinene cyclase was purified, and polyclonal antibodies were generated in rabbits against the sodium dodecyl sulfate-denatured protein. The antibody preparation was found to crossreact by Western blotting with other grand fir monoterpene cyclases that produce different olefinic products, but not with monoterpene cyclases from related conifer species (Pinus contorta and P. ponderosa) or from angiosperms (Mentha piperita and M. spicata). The increase in monoterpene cyclase activity after wounding was closely correlated with the appearance of new cyclase protein as determined by immunoblotting. These results indicate that the wound-dependent increase in monoterpene cyclase activity is a consequence of de novo synthesis of cyclase protein. © 1992.
Scientific Publication
You may also be interested in