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פותח על ידי קלירמאש פתרונות בע"מ -
Replication of Tomato Yellow Leaf Curl virus in its whitefly vector, Bemisia tabaci
Year:
2015
Source of publication :
Journal of Virology
Authors :
גנאים, מוראד
;
.
לבדב, גלינה
;
.
קונצדלוב, סבטלנה
;
.
Volume :
89
Co-Authors:
Pakkianathan, B.C., Institute of Plant Protection, Department of Entomology, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel
Kontsedalov, S., Institute of Plant Protection, Department of Entomology, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel
Lebedev, G., Institute of Plant Protection, Department of Entomology, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel
Mahadav, A., Institute of Plant Sciences and Genetics in Agriculture, Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel
Zeidan, M., Al-Qasemi Research Center, Baqa Elgharbia, Israel
Czosnek, H., Institute of Plant Sciences and Genetics in Agriculture, Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel
Ghanim, M., Institute of Plant Protection, Department of Entomology, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel
Facilitators :
From page:
9791
To page:
9803
(
Total pages:
13
)
Abstract:
Tomato yellow leaf curl virus (TYLCV) is a begomovirus transmitted exclusively by the whitefly Bemisia tabaci in a persistent, circulative manner. Replication of TYLCV in its vector remains controversial, and thus far, the virus has been considered to be nonpropagative. Following 8 h of acquisition on TYLCV-infected tomato plants or purified virions and then transfer to non- TYLCV-host cotton plants, the amounts of virus inside whitefly adults significantly increased (>2-fold) during the first few days and then continuously decreased, as measured by the amounts of genes on both virus DNA strands. Reported alterations in insect immune and defense responses upon virus retention led us to hypothesize a role for the immune response in suppressing virus replication. After virus acquisition, stress conditions were imposed on whiteflies, and the levels of three viral gene sequences were measured over time. When whiteflies were exposed to TYLCV and treatment with two different pesticides, the virus levels continuously increased. Upon exposure to heat stress, the virus levels gradually decreased, without any initial accumulation. Switching of whiteflies between pesticide, heat stress, and control treatments caused fluctuating increases and decreases in virus levels. Fluorescence in situ hybridization analysis confirmed these results and showed virus signals inside midgut epithelial cell nuclei. Combining the pesticide and heat treatments with virus acquisition had significant effects on fecundity. Altogether, our results demonstrate for the first time that a single-stranded DNA plant virus can replicate in its hemipteran vector. © 2015, American Society for Microbiology.
Note:
Related Files :
Animal
Animals
Genetics
heat stress
host resistance
pesticides
Tomato yellow leaf curl virus
Virology
virus gene
עוד תגיות
תוכן קשור
More details
DOI :
10.1128/JVI.00779-15
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
27720
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:33
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Scientific Publication
Replication of Tomato Yellow Leaf Curl virus in its whitefly vector, Bemisia tabaci
89
Pakkianathan, B.C., Institute of Plant Protection, Department of Entomology, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel
Kontsedalov, S., Institute of Plant Protection, Department of Entomology, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel
Lebedev, G., Institute of Plant Protection, Department of Entomology, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel
Mahadav, A., Institute of Plant Sciences and Genetics in Agriculture, Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel
Zeidan, M., Al-Qasemi Research Center, Baqa Elgharbia, Israel
Czosnek, H., Institute of Plant Sciences and Genetics in Agriculture, Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel
Ghanim, M., Institute of Plant Protection, Department of Entomology, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel
Replication of Tomato Yellow Leaf Curl virus in its whitefly vector, Bemisia tabaci
Tomato yellow leaf curl virus (TYLCV) is a begomovirus transmitted exclusively by the whitefly Bemisia tabaci in a persistent, circulative manner. Replication of TYLCV in its vector remains controversial, and thus far, the virus has been considered to be nonpropagative. Following 8 h of acquisition on TYLCV-infected tomato plants or purified virions and then transfer to non- TYLCV-host cotton plants, the amounts of virus inside whitefly adults significantly increased (>2-fold) during the first few days and then continuously decreased, as measured by the amounts of genes on both virus DNA strands. Reported alterations in insect immune and defense responses upon virus retention led us to hypothesize a role for the immune response in suppressing virus replication. After virus acquisition, stress conditions were imposed on whiteflies, and the levels of three viral gene sequences were measured over time. When whiteflies were exposed to TYLCV and treatment with two different pesticides, the virus levels continuously increased. Upon exposure to heat stress, the virus levels gradually decreased, without any initial accumulation. Switching of whiteflies between pesticide, heat stress, and control treatments caused fluctuating increases and decreases in virus levels. Fluorescence in situ hybridization analysis confirmed these results and showed virus signals inside midgut epithelial cell nuclei. Combining the pesticide and heat treatments with virus acquisition had significant effects on fecundity. Altogether, our results demonstrate for the first time that a single-stranded DNA plant virus can replicate in its hemipteran vector. © 2015, American Society for Microbiology.
Scientific Publication
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