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פותח על ידי קלירמאש פתרונות בע"מ -
Cell hierarchy and lineage commitment in the bovine mammary gland
Year:
2012
Source of publication :
PLoS ONE
Authors :
ברש, איתמר
;
.
Volume :
7
Co-Authors:
Rauner, G., Institute of Animal Science, ARO, The Volcani Center, Bet-Dagan, Israel, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Jerusalem, Israel
Barash, I., Institute of Animal Science, ARO, The Volcani Center, Bet-Dagan, Israel
Facilitators :
From page:
To page:
(
Total pages:
1
)
Abstract:
The bovine mammary gland is a favorable organ for studying mammary cell hierarchy due to its robust milk-production capabilities that reflect the adaptation of its cell populations to extensive expansion and differentiation. It also shares basic characteristics with the human breast, and identification of its cell composition may broaden our understanding of the diversity in cell hierarchy among mammals. Here, Lin - epithelial cells were sorted according to expression of CD24 and CD49f into four populations: CD24 medCD49f pos (putative stem cells, puStm), CD24 negCD49f pos (Basal), CD24 highCD49f neg (putative progenitors, puPgt) and CD24 medCD49f neg (luminal, Lum). These populations maintained differential gene expression of lineage markers and markers of stem cells and luminal progenitors. Of note was the high expression of Stat5a in the puPgt cells, and of Notch1, Delta1, Jagged1 and Hey1 in the puStm and Basal populations. Cultured puStm and Basal cells formed lineage-restricted basal or luminal clones and after re-sorting, colonies that preserved a duct-like alignment of epithelial layers. In contrast, puPgt and Lum cells generated only luminal clones and unorganized colonies. Under non-adherent culture conditions, the puPgt and puStm populations generated significantly more floating colonies. The increase in cell number during culture provides a measure of propagation potential, which was highest for the puStm cells. Taken together, these analyses position puStm cells at the top of the cell hierarchy and denote the presence of both bi-potent and luminally restricted progenitors. In addition, a population of differentiated luminal cells was marked. Finally, combining ALDH activity with cell-surface marker analyses defined a small subpopulation that is potentially stem cell- enriched. © 2012 Rauner, Barash.
Note:
Related Files :
Animal
Animals
animal tissue
cattle
Female
Mammalia
metabolism
עוד תגיות
תוכן קשור
More details
DOI :
10.1371/journal.pone.0030113
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
27731
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:33
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Scientific Publication
Cell hierarchy and lineage commitment in the bovine mammary gland
7
Rauner, G., Institute of Animal Science, ARO, The Volcani Center, Bet-Dagan, Israel, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Jerusalem, Israel
Barash, I., Institute of Animal Science, ARO, The Volcani Center, Bet-Dagan, Israel
Cell hierarchy and lineage commitment in the bovine mammary gland
The bovine mammary gland is a favorable organ for studying mammary cell hierarchy due to its robust milk-production capabilities that reflect the adaptation of its cell populations to extensive expansion and differentiation. It also shares basic characteristics with the human breast, and identification of its cell composition may broaden our understanding of the diversity in cell hierarchy among mammals. Here, Lin - epithelial cells were sorted according to expression of CD24 and CD49f into four populations: CD24 medCD49f pos (putative stem cells, puStm), CD24 negCD49f pos (Basal), CD24 highCD49f neg (putative progenitors, puPgt) and CD24 medCD49f neg (luminal, Lum). These populations maintained differential gene expression of lineage markers and markers of stem cells and luminal progenitors. Of note was the high expression of Stat5a in the puPgt cells, and of Notch1, Delta1, Jagged1 and Hey1 in the puStm and Basal populations. Cultured puStm and Basal cells formed lineage-restricted basal or luminal clones and after re-sorting, colonies that preserved a duct-like alignment of epithelial layers. In contrast, puPgt and Lum cells generated only luminal clones and unorganized colonies. Under non-adherent culture conditions, the puPgt and puStm populations generated significantly more floating colonies. The increase in cell number during culture provides a measure of propagation potential, which was highest for the puStm cells. Taken together, these analyses position puStm cells at the top of the cell hierarchy and denote the presence of both bi-potent and luminally restricted progenitors. In addition, a population of differentiated luminal cells was marked. Finally, combining ALDH activity with cell-surface marker analyses defined a small subpopulation that is potentially stem cell- enriched. © 2012 Rauner, Barash.
Scientific Publication
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