אסיף מאגר המחקר החקלאי

A reliable and rapid shaking extraction system for determination of the oil content in olive fruit

Year:

1999

Source of publication :

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Volume :

474

Co-Authors:

Avidan, B., ARO, Institue of Horticulture, Volcani Center, Bet Dagan, Israel
Ogrodovitch, A., ARO, Institue of Horticulture, Volcani Center, Bet Dagan, Israel
Lavee, S., ARO, Institue of Horticulture, Volcani Center, Bet Dagan, Israel

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Abstract:

The quantitative determination of the oil content in olive fruits on a large scale is important for both the industry and research. The standard methods today are either slow and the rapid ones involve health hazards for the technicians or require expensive equipment. Thus a simple and rapid method for the reliable determination of the oil content in olive mesocarp was developed. For each sample, 5g of fresh mesocarp is dried for 24h at 80°C, followed by homogenisation and extraction with 10ml petroleum ether 60-80°C grade. The crude extract is transferred to scintillation vials and shaken overnight. The samples are then paper filtered using a Bitchier apparatus connected to a small laboratory pump. The clean exude is evaporated at 40°C and the remainder oil weighed. Results were presented as percent oil of fresh and dry mesocarp weight and were found to be highly correlated with the standard Soxhlet oil extraction method. The standard Soxhelt method for oil determination in the tissue, suffers from drawbacks for large scale sampling due to its relative slowness and large lab space requirements. On the other hand the refractometric method developed for this purpose is no longer safe for standard use in the laboratory because of the carcinogenic hazard of the major extraction solvent, chloronaphthalene, used in that method. The advantages of this modified Soxhlet system are its simplicity, reliability, friendliness to environment, rapid, inexpensive and thus very suitable for the oil quantification in a large number of fruit mesocarp samples. The method might be suitable for using in commercial oil mills for determining the oil content in the incoming fruit loads of fruit.

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