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חיפוש מתקדם
Theoretical and Applied Genetics
Sharon, D., Dept. Fruit Trees, Genet. and Breed., Agricultural Research Organization, P.O. Box 6, Bet-Dagan 50250, Israel
Cregan, P.B., USDA, ARS, Soybean and Alfalfa Res. Laboratory, Beltsville, MD 20705, United States
Mhameed, S., Dept. Fruit Trees, Genet. and Breed., Agricultural Research Organization, P.O. Box 6, Bet-Dagan 50250, Israel
Kusharska, M., Department of Genetics, Silesian University, Jagiallonska 28, Katovice 40032, Poland
Hillel, J., Department of Genetics, Faculty of Agriculture, Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
Lahav, E., Dept. Fruit Trees, Genet. and Breed., Agricultural Research Organization, P.O. Box 6, Bet-Dagan 50250, Israel
Lavi, U., Dept. Fruit Trees, Genet. and Breed., Agricultural Research Organization, P.O. Box 6, Bet-Dagan 50250, Israel
An avocado genomic library was screened with various microsatellite repeats. (A/T)(n) and (TC/AG)(n) sequences were found to be the most frequent repeats. One hundred and seventy-two positive clones were sequenced successfully of which 113 were found to contain simple sequence repeats (SSR). Polymerase chain reaction primers were designed to the regions flanking the SSR in 62 clones. A GenBank search of avocado DNA sequences revealed 1 sequence containing a (CT)10 repeat. A total of 92 avocado-specific SSR markers were screened for polymorphism using 50 offspring of a cross between the avocado cultivars 'Pinkerton' and 'Ettinger'. Both are standard avocado cultivars which are normally outcrossed and highly heterozygous. Fifty polymorphic SSR loci, 17 random amplified polymorphic DNA (RAPD) and 23 minisatellite DNA Fingerprint (DFP) bands were used to construct the avocado genetic map. The resulting data were analyzed with various mapping programs in order to assess which program best accommodated data from progeny of heterozygous parents. The analyses resulted in 12 linkage groups with 34 markers (25 SSRs, 3 RAPDs and 6 DFP bands) covering 352.6 cM. This initial map can serve as a basis for developing a detailed genomic map and for detection of linkage between markers and quantitative trait loci.
פותח על ידי קלירמאש פתרונות בע"מ -
הספר "אוצר וולקני"
אודות
תנאי שימוש
An integrated genetic linkage map of avocado
95
Sharon, D., Dept. Fruit Trees, Genet. and Breed., Agricultural Research Organization, P.O. Box 6, Bet-Dagan 50250, Israel
Cregan, P.B., USDA, ARS, Soybean and Alfalfa Res. Laboratory, Beltsville, MD 20705, United States
Mhameed, S., Dept. Fruit Trees, Genet. and Breed., Agricultural Research Organization, P.O. Box 6, Bet-Dagan 50250, Israel
Kusharska, M., Department of Genetics, Silesian University, Jagiallonska 28, Katovice 40032, Poland
Hillel, J., Department of Genetics, Faculty of Agriculture, Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
Lahav, E., Dept. Fruit Trees, Genet. and Breed., Agricultural Research Organization, P.O. Box 6, Bet-Dagan 50250, Israel
Lavi, U., Dept. Fruit Trees, Genet. and Breed., Agricultural Research Organization, P.O. Box 6, Bet-Dagan 50250, Israel
An integrated genetic linkage map of avocado
An avocado genomic library was screened with various microsatellite repeats. (A/T)(n) and (TC/AG)(n) sequences were found to be the most frequent repeats. One hundred and seventy-two positive clones were sequenced successfully of which 113 were found to contain simple sequence repeats (SSR). Polymerase chain reaction primers were designed to the regions flanking the SSR in 62 clones. A GenBank search of avocado DNA sequences revealed 1 sequence containing a (CT)10 repeat. A total of 92 avocado-specific SSR markers were screened for polymorphism using 50 offspring of a cross between the avocado cultivars 'Pinkerton' and 'Ettinger'. Both are standard avocado cultivars which are normally outcrossed and highly heterozygous. Fifty polymorphic SSR loci, 17 random amplified polymorphic DNA (RAPD) and 23 minisatellite DNA Fingerprint (DFP) bands were used to construct the avocado genetic map. The resulting data were analyzed with various mapping programs in order to assess which program best accommodated data from progeny of heterozygous parents. The analyses resulted in 12 linkage groups with 34 markers (25 SSRs, 3 RAPDs and 6 DFP bands) covering 352.6 cM. This initial map can serve as a basis for developing a detailed genomic map and for detection of linkage between markers and quantitative trait loci.
Scientific Publication
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