חיפוש מתקדם
microbial ecology (source)
Singh, S.K., Department of Plant Sciences, Montana State University, Bozeman, MT 59717, United States, National Facility for Culture Collection of Fungi, Mycology and Plant Pathology Group, MACS'Agharkar Research Institute, Pune 411004, India
Strobel, G.A., Department of Plant Sciences, Montana State University, Bozeman, MT 59717, United States
Knighton, B., Department of Chemistry and Biochemistry, Montana State University, Bozeman, MT 59717, United States
Geary, B., Department of Plant and Wildlife Sciences, Brigham Young University, Provo, UT 84602, United States
Sears, J., Center for Lab Services/RJ Lee Group, 2710 North 20th Ave, Pasco, WA 99301, United States
Ezra, D., Department of Plant Pathology and Weed Research, ARO, The Volcani Center, P.O.B. 6, Bet-Dagan 50250, Israel
An unusual Phomopsis sp. was isolated as endophyte of Odontoglossum sp. (Orchidaceae), associated with a cloud forest in Northern Ecuador. This fungus produces a unique mixture of volatile organic compounds (VOCs) including sabinene (a monoterpene with a peppery odor) only previously known from higher plants. In addition, some of the other more abundant VOCs recorded by GC/MS in this organism were 1-butanol, 3-methyl; benzeneethanol; 1-propanol, 2-methyl and 2-propanone. The gases of Phomopsis sp. possess antifungal properties and an artificial mixture of the VOCs mimicked the antibiotic effects of this organism with the greatest bioactivity against a wide range of plant pathogenic test fungi including: Pythium, Phytophthora, Sclerotinia, Rhizoctonia, Fusarium, Botrytis, Verticillium, and Colletotrichum. The IC50 values for the artificial gas mixture of Phomopsis sp. varied between 8 and 25.65 μl/mL. Proton transfer reaction-mass spectrometry monitored the concentration of VOCs emitted by Phomopsis sp. and yielded a total VOC concentration of ca. 18 ppmv in the head space at the seventh day of incubation at 23°C on PDA. As with many VOC-producing endophytes, this Phomopsis sp. did survive and grow in the presence of the inhibitory gases of Muscodor albus. A discussion is presented on the possible involvement of VOC production by the fungus and its role in the biology/ecology of the fungus/plant/environmental relationship. © 2011 Springer Science+Business Media, LLC.
פותח על ידי קלירמאש פתרונות בע"מ -
הספר "אוצר וולקני"
אודות
תנאי שימוש
An Endophytic Phomopsis sp. Possessing Bioactivity and Fuel Potential with its Volatile Organic Compounds
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Singh, S.K., Department of Plant Sciences, Montana State University, Bozeman, MT 59717, United States, National Facility for Culture Collection of Fungi, Mycology and Plant Pathology Group, MACS'Agharkar Research Institute, Pune 411004, India
Strobel, G.A., Department of Plant Sciences, Montana State University, Bozeman, MT 59717, United States
Knighton, B., Department of Chemistry and Biochemistry, Montana State University, Bozeman, MT 59717, United States
Geary, B., Department of Plant and Wildlife Sciences, Brigham Young University, Provo, UT 84602, United States
Sears, J., Center for Lab Services/RJ Lee Group, 2710 North 20th Ave, Pasco, WA 99301, United States
Ezra, D., Department of Plant Pathology and Weed Research, ARO, The Volcani Center, P.O.B. 6, Bet-Dagan 50250, Israel
An Endophytic Phomopsis sp. Possessing Bioactivity and Fuel Potential with its Volatile Organic Compounds
An unusual Phomopsis sp. was isolated as endophyte of Odontoglossum sp. (Orchidaceae), associated with a cloud forest in Northern Ecuador. This fungus produces a unique mixture of volatile organic compounds (VOCs) including sabinene (a monoterpene with a peppery odor) only previously known from higher plants. In addition, some of the other more abundant VOCs recorded by GC/MS in this organism were 1-butanol, 3-methyl; benzeneethanol; 1-propanol, 2-methyl and 2-propanone. The gases of Phomopsis sp. possess antifungal properties and an artificial mixture of the VOCs mimicked the antibiotic effects of this organism with the greatest bioactivity against a wide range of plant pathogenic test fungi including: Pythium, Phytophthora, Sclerotinia, Rhizoctonia, Fusarium, Botrytis, Verticillium, and Colletotrichum. The IC50 values for the artificial gas mixture of Phomopsis sp. varied between 8 and 25.65 μl/mL. Proton transfer reaction-mass spectrometry monitored the concentration of VOCs emitted by Phomopsis sp. and yielded a total VOC concentration of ca. 18 ppmv in the head space at the seventh day of incubation at 23°C on PDA. As with many VOC-producing endophytes, this Phomopsis sp. did survive and grow in the presence of the inhibitory gases of Muscodor albus. A discussion is presented on the possible involvement of VOC production by the fungus and its role in the biology/ecology of the fungus/plant/environmental relationship. © 2011 Springer Science+Business Media, LLC.
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