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פותח על ידי קלירמאש פתרונות בע"מ -
5′-Coterminal subgenomic RNAS in citrus tristeza virus-infected cells
Year:
2001
Source of publication :
Virology
Authors :
בר-יוסף, משה
;
.
יאנג, גואנג
;
.
מוואסי, מוניר
;
.
פיאסטון, דן
;
.
צה, קסי בין
;
.
Volume :
283
Co-Authors:
Che, X., S. Tolkowsky Laboratory, Department of Virology, Volcani Center, Bet Dagan, Israel
Piestun, D., S. Tolkowsky Laboratory, Department of Virology, Volcani Center, Bet Dagan, Israel
Mawassi, M., S. Tolkowsky Laboratory, Department of Virology, Volcani Center, Bet Dagan, Israel
Yang, G., S. Tolkowsky Laboratory, Department of Virology, Volcani Center, Bet Dagan, Israel
Satyanarayana, T., University of Florida, CREC, Lake Alfred, FL 33850, United States
Gowda, S., University of Florida, CREC, Lake Alfred, FL 33850, United States
Dawson, W.O., University of Florida, CREC, Lake Alfred, FL 33850, United States
Bar-Joseph, M., S. Tolkowsky Laboratory, Department of Virology, Volcani Center, Bet Dagan, Israel
Facilitators :
From page:
374
To page:
381
(
Total pages:
8
)
Abstract:
Three unusual 5′ coterminal positive-stranded subgenomic (sg) RNAs, two of about 0.8 kb and one of 10 kb (designated LMT1, LMT2, and LaMT, respectively), from Citrus spp. plants and Nicotiana benthamiana protoplasts infected with Citrus tristeza virus (CTV) were characterized. The 5′ termini of the LMT RNAs were mapped by runoff reverse transcription and found to correspond with the 5′ terminus of the genomic RNA. The LMT 5′-coterminal sgRNAs consisted of two modal lengths of 744-746 and 842-854 nts. The 3′ of the LaMT RNAs terminated near the junction of ORF 1b and ORF 2 (p33). None of the 5′ sgRNAs had detectable amounts of corresponding negative-sense RNAs, as occurs with the genomic and 3′ coterminal subgenomic RNAs of CTV. The abundance of the short and long 5′ sgRNAs differed considerably in infected cells. The LMT RNAs were considerably more abundant than the genomic RNAs, while the larger LaMT RNA accumulated to much lower levels. The kinetics of accumulation of LMT1 and LMT2 in synchronously infected protoplasts differed. The larger RNA, LMT1, accumulated earlier with a strong hybridization signal at 2 days postinfection, a time when only traces of genomic and 3′ sgRNAs were detected. The lack of corresponding RNAs, that could be 3′ cleavage products corresponding to the 5′ coterminal sgRNAs and the lack of complementary negative strands, suggest that these sgRNAs were produced by termination during the synthesis of the genomic positive strands. © 2001 Academic Press.
Note:
Related Files :
Citrus tristeza virus
genomics
hybridization
Open reading frame
RNA
virus genome
virus RNA
עוד תגיות
תוכן קשור
More details
DOI :
10.1006/viro.2001.0880
Article number:
0
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
27900
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:35
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Scientific Publication
5′-Coterminal subgenomic RNAS in citrus tristeza virus-infected cells
283
Che, X., S. Tolkowsky Laboratory, Department of Virology, Volcani Center, Bet Dagan, Israel
Piestun, D., S. Tolkowsky Laboratory, Department of Virology, Volcani Center, Bet Dagan, Israel
Mawassi, M., S. Tolkowsky Laboratory, Department of Virology, Volcani Center, Bet Dagan, Israel
Yang, G., S. Tolkowsky Laboratory, Department of Virology, Volcani Center, Bet Dagan, Israel
Satyanarayana, T., University of Florida, CREC, Lake Alfred, FL 33850, United States
Gowda, S., University of Florida, CREC, Lake Alfred, FL 33850, United States
Dawson, W.O., University of Florida, CREC, Lake Alfred, FL 33850, United States
Bar-Joseph, M., S. Tolkowsky Laboratory, Department of Virology, Volcani Center, Bet Dagan, Israel
5′-Coterminal subgenomic RNAS in citrus tristeza virus-infected cells
Three unusual 5′ coterminal positive-stranded subgenomic (sg) RNAs, two of about 0.8 kb and one of 10 kb (designated LMT1, LMT2, and LaMT, respectively), from Citrus spp. plants and Nicotiana benthamiana protoplasts infected with Citrus tristeza virus (CTV) were characterized. The 5′ termini of the LMT RNAs were mapped by runoff reverse transcription and found to correspond with the 5′ terminus of the genomic RNA. The LMT 5′-coterminal sgRNAs consisted of two modal lengths of 744-746 and 842-854 nts. The 3′ of the LaMT RNAs terminated near the junction of ORF 1b and ORF 2 (p33). None of the 5′ sgRNAs had detectable amounts of corresponding negative-sense RNAs, as occurs with the genomic and 3′ coterminal subgenomic RNAs of CTV. The abundance of the short and long 5′ sgRNAs differed considerably in infected cells. The LMT RNAs were considerably more abundant than the genomic RNAs, while the larger LaMT RNA accumulated to much lower levels. The kinetics of accumulation of LMT1 and LMT2 in synchronously infected protoplasts differed. The larger RNA, LMT1, accumulated earlier with a strong hybridization signal at 2 days postinfection, a time when only traces of genomic and 3′ sgRNAs were detected. The lack of corresponding RNAs, that could be 3′ cleavage products corresponding to the 5′ coterminal sgRNAs and the lack of complementary negative strands, suggest that these sgRNAs were produced by termination during the synthesis of the genomic positive strands. © 2001 Academic Press.
Scientific Publication
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