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פותח על ידי קלירמאש פתרונות בע"מ -
Osteocalcin (BGP), gene expression, and protein production by marrow stromal adipocytes
Year:
1997
Authors :
שמאי, אבי
;
.
Volume :
231
Co-Authors:
Benayahu, D., Dept. of Cell Biology and Histology, Sackler Faculty of Medicine, Tel-Aviv University, Tel-Aviv 66978, Israel
Shamay, A., Division of Orthopaedics, Sackler Faculty of Medicine, Tel-Aviv University, Tel-Aviv 66978, Israel
Wientroub, S., Division of Orthopaedics, Sackler Faculty of Medicine, Tel-Aviv University, Tel-Aviv 66978, Israel, Dept. of Pediatric Orthopaedics, Dana Children's Hospital, Tel-Aviv Medical Center, Tel-Aviv 64239, Israel
Facilitators :
From page:
442
To page:
446
(
Total pages:
5
)
Abstract:
This study was designed to demonstrate the expression and production of osteocalcin, a bone Gla-protein (BGP), by marrow stromal cells. We were able to accomplish this by using a series of marrow stromal cell lines (MBA cells). A unique expression of the osteocalcin was detected by the adipocyte 14F1.1 cells. This was at the mRNA level by Northern blot and by RT-PCR analysis. The secreted protein was quantitated by radioimmunoassay (RIA), in conditioned medium (CM) harvested from these cultured cells. These findings offer the first evidence that marrow adipocyte 14F1.1 derived cells express mRNA for osteocalcin and produce the protein.
Note:
Related Files :
Animals
bone marrow
cell line
gene expression
mice
Polymerase Chain Reaction
protein synthesis
radioimmunoassay
עוד תגיות
תוכן קשור
More details
DOI :
10.1006/bbrc.1997.6116
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
27904
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:35
Scientific Publication
Osteocalcin (BGP), gene expression, and protein production by marrow stromal adipocytes
231
Benayahu, D., Dept. of Cell Biology and Histology, Sackler Faculty of Medicine, Tel-Aviv University, Tel-Aviv 66978, Israel
Shamay, A., Division of Orthopaedics, Sackler Faculty of Medicine, Tel-Aviv University, Tel-Aviv 66978, Israel
Wientroub, S., Division of Orthopaedics, Sackler Faculty of Medicine, Tel-Aviv University, Tel-Aviv 66978, Israel, Dept. of Pediatric Orthopaedics, Dana Children's Hospital, Tel-Aviv Medical Center, Tel-Aviv 64239, Israel
Osteocalcin (BGP), gene expression, and protein production by marrow stromal adipocytes
This study was designed to demonstrate the expression and production of osteocalcin, a bone Gla-protein (BGP), by marrow stromal cells. We were able to accomplish this by using a series of marrow stromal cell lines (MBA cells). A unique expression of the osteocalcin was detected by the adipocyte 14F1.1 cells. This was at the mRNA level by Northern blot and by RT-PCR analysis. The secreted protein was quantitated by radioimmunoassay (RIA), in conditioned medium (CM) harvested from these cultured cells. These findings offer the first evidence that marrow adipocyte 14F1.1 derived cells express mRNA for osteocalcin and produce the protein.
Scientific Publication
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