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An in vitro model to study interactions between Escherichia coli and lactic acid bacterial inoculants for silage in rumen fluid
Year:
2016
Source of publication :
Letters in Applied Microbiology
Authors :
וולצ'ינסקי, ו'
;
.
וינברג, צבי
;
.
חן, יאירה
;
.
סלע, שלמה
;
.
Volume :
63
Co-Authors:
Weinberg, Z.G., Department of Food Quality and Safety, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel
Chen, Y., Department of Food Quality and Safety, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel
Volchinski, V., Department of Food Quality and Safety, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel
Sela, S., Department of Food Quality and Safety, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel
Ogunade, I.M., Department of Animal Science, University of Florida, Gainesville, FL, United States
Adesogan, A., Department of Animal Science, University of Florida, Gainesville, FL, United States
Facilitators :
From page:
60
To page:
65
(
Total pages:
6
)
Abstract:
Previous studies have shown that silages treated with lactic acid bacteria (LAB) inoculants enhance ruminants’ performance. The objective of the current experiments was to develop an in vitro model to study interactions between LAB silage inoculants and inoculated silages and Escherichia coli (EC) in rumen fluid (RF). Our hypothesis was that some inoculants inhibit EC in RF. For that purpose buffered RF was incubated under anaerobic conditions at 39°C with commercial strains of LAB silage inoculants or with laboratory corn and wheat silages treated with these LAB, an EC strain and with various ruminant feed ingredients. The EC strain was originally isolated from cattle manure and tagged with a plasmid expressing the green fluorescence protein and kanamycin and streptomycin resistance. Results indicate that the LAB or the treated silages did not suppress EC numbers in the RF. When the pH of the RF decreased below 5·0 the EC disappeared. We conclude that both LAB inoculants for silage and EC survived in RF for several days; however, the inoculants and silages treated with such inoculants did not inhibit EC in RF in vitro. Significance and Impact of the Study: Forage crops, silage and hay are initial stages of the food chain for humans. Cattle harbours and sheds enterobacteria regularly, some strains of which are pathogens. These can contaminate forage crops through field fertilization with cattle manure. The objective of this study was to develop an in vitro model to test whether lactic acid bacteria, which are used in silage inoculants, alone or in treated silages can inhibit Escherichia coli in rumen fluid. This study presents safety aspects and it is also part of a broad research effort aimed at finding out how LAB silage inoculants and inoculated silages enhance ruminant performance or exert probiotic effects in ruminants. © 2016 The Society for Applied Microbiology
Note:
Related Files :
anoxic conditions
coliform bacterium
Plasmid
probiotics
Ruminant
Triticum aestivum
Zea mays
עוד תגיות
תוכן קשור
More details
DOI :
10.1111/lam.12587
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
28071
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:36
Scientific Publication
An in vitro model to study interactions between Escherichia coli and lactic acid bacterial inoculants for silage in rumen fluid
63
Weinberg, Z.G., Department of Food Quality and Safety, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel
Chen, Y., Department of Food Quality and Safety, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel
Volchinski, V., Department of Food Quality and Safety, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel
Sela, S., Department of Food Quality and Safety, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel
Ogunade, I.M., Department of Animal Science, University of Florida, Gainesville, FL, United States
Adesogan, A., Department of Animal Science, University of Florida, Gainesville, FL, United States
An in vitro model to study interactions between Escherichia coli and lactic acid bacterial inoculants for silage in rumen fluid
Previous studies have shown that silages treated with lactic acid bacteria (LAB) inoculants enhance ruminants’ performance. The objective of the current experiments was to develop an in vitro model to study interactions between LAB silage inoculants and inoculated silages and Escherichia coli (EC) in rumen fluid (RF). Our hypothesis was that some inoculants inhibit EC in RF. For that purpose buffered RF was incubated under anaerobic conditions at 39°C with commercial strains of LAB silage inoculants or with laboratory corn and wheat silages treated with these LAB, an EC strain and with various ruminant feed ingredients. The EC strain was originally isolated from cattle manure and tagged with a plasmid expressing the green fluorescence protein and kanamycin and streptomycin resistance. Results indicate that the LAB or the treated silages did not suppress EC numbers in the RF. When the pH of the RF decreased below 5·0 the EC disappeared. We conclude that both LAB inoculants for silage and EC survived in RF for several days; however, the inoculants and silages treated with such inoculants did not inhibit EC in RF in vitro. Significance and Impact of the Study: Forage crops, silage and hay are initial stages of the food chain for humans. Cattle harbours and sheds enterobacteria regularly, some strains of which are pathogens. These can contaminate forage crops through field fertilization with cattle manure. The objective of this study was to develop an in vitro model to test whether lactic acid bacteria, which are used in silage inoculants, alone or in treated silages can inhibit Escherichia coli in rumen fluid. This study presents safety aspects and it is also part of a broad research effort aimed at finding out how LAB silage inoculants and inoculated silages enhance ruminant performance or exert probiotic effects in ruminants. © 2016 The Society for Applied Microbiology
Scientific Publication
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