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פותח על ידי קלירמאש פתרונות בע"מ -
Unusual sequence relationships between two isolates of citrus tristeza virus
Year:
1996
Source of publication :
Journal of General Virology
Authors :
בר-יוסף, משה
;
.
גופמן, רוז
;
.
יאנג, גואנג
;
.
מוואסי, מוניר
;
.
מיטקיבסקה, אליזבטה
;
.
Volume :
77
Co-Authors:
Mawassi, M., S. Tolkowsky Laboratory, Department of Virology, Volcani Center, Bet Dagan 50250, Israel
Mietkiewska, E., S. Tolkowsky Laboratory, Department of Virology, Volcani Center, Bet Dagan 50250, Israel
Gofman, R., S. Tolkowsky Laboratory, Department of Virology, Volcani Center, Bet Dagan 50250, Israel
Yang, G., S. Tolkowsky Laboratory, Department of Virology, Volcani Center, Bet Dagan 50250, Israel
Bar-Joseph, M., S. Tolkowsky Laboratory, Department of Virology, Volcani Center, Bet Dagan 50250, Israel
Facilitators :
From page:
2359
To page:
2364
(
Total pages:
6
)
Abstract:
The complete, 19226 nt sequence of the RNA genome from VT, a seedling yellows strain of citrus tristeza virus (CTV), was determined and found to have a genome organization identical with that of the previously determined CTV-T36 isolate, except that ORF 1 of CTV-VT was 70 nt shorter due to two widely separated 18 nt deletions. Sequence comparison of CTV-VT and CTV-T36 revealed approximately 89% identity throughout the ten 3' ORFs, but only 60-70% identity throughout ORF 1. The 5' nontranslated regions were only 60% identical whereas the 3' nontranslated regions were 97% identical. The transition between regions of similarity and deviation was gradual, suggesting that the sequence similarities and differences compared to CTV-T36 were unlikely to have arisen from a recent recombination event between a close T36 relative and a distantly related CTV isolate. This is the first attempt to compare in detail the variation between the genomes of two strains of a member of the closterovirus group. The observed deviation between the large RNA genomes of the two CTV strains is greater than that among different viruses of most other groups, raising the question of how to define the taxonomy of these viruses.
Note:
Related Files :
Base Sequence
citrus fruit
Citrus tristeza virus
Closterovirus
Genome, Viral
Molecular Sequence Data
sequence homology
virus RNA
עוד תגיות
תוכן קשור
More details
DOI :
Article number:
0
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
28092
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:36
You may also be interested in
Scientific Publication
Unusual sequence relationships between two isolates of citrus tristeza virus
77
Mawassi, M., S. Tolkowsky Laboratory, Department of Virology, Volcani Center, Bet Dagan 50250, Israel
Mietkiewska, E., S. Tolkowsky Laboratory, Department of Virology, Volcani Center, Bet Dagan 50250, Israel
Gofman, R., S. Tolkowsky Laboratory, Department of Virology, Volcani Center, Bet Dagan 50250, Israel
Yang, G., S. Tolkowsky Laboratory, Department of Virology, Volcani Center, Bet Dagan 50250, Israel
Bar-Joseph, M., S. Tolkowsky Laboratory, Department of Virology, Volcani Center, Bet Dagan 50250, Israel
Unusual sequence relationships between two isolates of citrus tristeza virus
The complete, 19226 nt sequence of the RNA genome from VT, a seedling yellows strain of citrus tristeza virus (CTV), was determined and found to have a genome organization identical with that of the previously determined CTV-T36 isolate, except that ORF 1 of CTV-VT was 70 nt shorter due to two widely separated 18 nt deletions. Sequence comparison of CTV-VT and CTV-T36 revealed approximately 89% identity throughout the ten 3' ORFs, but only 60-70% identity throughout ORF 1. The 5' nontranslated regions were only 60% identical whereas the 3' nontranslated regions were 97% identical. The transition between regions of similarity and deviation was gradual, suggesting that the sequence similarities and differences compared to CTV-T36 were unlikely to have arisen from a recent recombination event between a close T36 relative and a distantly related CTV isolate. This is the first attempt to compare in detail the variation between the genomes of two strains of a member of the closterovirus group. The observed deviation between the large RNA genomes of the two CTV strains is greater than that among different viruses of most other groups, raising the question of how to define the taxonomy of these viruses.
Scientific Publication
You may also be interested in