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פותח על ידי קלירמאש פתרונות בע"מ -
The intracellular source, composition and regulatory functions of nanosized vesicles from bovine milk-serum
Year:
2015
Source of publication :
RSC Advances
Authors :
מרין, עוזי
;
.
סילניקוב, ניסים
;
.
שפירו, פירה
;
.
Volume :
5
Co-Authors:
Silanikove, N., Biology of Lactation Laboratory, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Shapiro, F., Biology of Lactation Laboratory, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Merin, U., Department of Food Quality and Safety, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan, Israel
Leitner, G., National Mastitis Reference Center, Kimron Veterinary Institute, P.O. Box 12, Bet Dagan, Israel
Facilitators :
From page:
69161
To page:
69175
(
Total pages:
15
)
Abstract:
A hypothesis that the source of milk-serum derived vesicles (MSDVs) is the Golgi apparatus (GA) was examined. Using dynamic light scattering and electron microscopy, it was shown that MSDVs are composed of globular structures with hydrodynamic sizes of 70 ± 15 nm. More than 60% of the total protein content of MSDVs was associated with the MSDV lumen and 30% was associated with the MSDV membrane. Casein was the major protein found in the MSDV lumen. The conclusive markers of the GA, lactose synthase components (α-lactalbumin and galactosyltransferase) and activity (synthesis of lactose from glucose and UTP-galactose), the presence of casein in micellar form in the MSDV lumen, and a high luminal content of citric acid, were demonstrated in the lumen of MSDVs. Though MSDVs composed only 0.7% of the milk mass, they accounted for a high proportion of the total milk content of reactive (15% Cu and 18% Fe) and toxic minerals (60% Cd and 65% Pb), which strongly suggests that MSDVs serve as an avenue to protect mammary epithelial cells from the toxic effects of these minerals by storing them intraluminally and secreting them into milk. The presence of micellar casein in the MSDV lumen, along with the presence of metal transporters in their membranes, is responsible for this impressive capacity for storing reactive and toxic minerals. Exposing a single mammary gland to lipopolysaccharide challenge induced changes in regulatory proteins stored in the lumen of MSDVs (tissue plasminogen activators, plasminogen and plasmin) and in the activity of xanthine oxidase and alkaline phosphatase attached to the outer membrane of MSDVs. Thus, we have demonstrated that MSDVs are under the regulation of the nucleus and respond to extracellular signals. © The Royal Society of Chemistry 2015.
Note:
Related Files :
Body Fluids
enzyme activity
Extracellular signals
Mammary epithelial cells
Minerals
Phosphatases
proteins
Total protein contents
עוד תגיות
תוכן קשור
More details
DOI :
10.1039/c5ra07599h
Article number:
0
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
28099
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:36
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Scientific Publication
The intracellular source, composition and regulatory functions of nanosized vesicles from bovine milk-serum
5
Silanikove, N., Biology of Lactation Laboratory, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Shapiro, F., Biology of Lactation Laboratory, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Merin, U., Department of Food Quality and Safety, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan, Israel
Leitner, G., National Mastitis Reference Center, Kimron Veterinary Institute, P.O. Box 12, Bet Dagan, Israel
The intracellular source, composition and regulatory functions of nanosized vesicles from bovine milk-serum
A hypothesis that the source of milk-serum derived vesicles (MSDVs) is the Golgi apparatus (GA) was examined. Using dynamic light scattering and electron microscopy, it was shown that MSDVs are composed of globular structures with hydrodynamic sizes of 70 ± 15 nm. More than 60% of the total protein content of MSDVs was associated with the MSDV lumen and 30% was associated with the MSDV membrane. Casein was the major protein found in the MSDV lumen. The conclusive markers of the GA, lactose synthase components (α-lactalbumin and galactosyltransferase) and activity (synthesis of lactose from glucose and UTP-galactose), the presence of casein in micellar form in the MSDV lumen, and a high luminal content of citric acid, were demonstrated in the lumen of MSDVs. Though MSDVs composed only 0.7% of the milk mass, they accounted for a high proportion of the total milk content of reactive (15% Cu and 18% Fe) and toxic minerals (60% Cd and 65% Pb), which strongly suggests that MSDVs serve as an avenue to protect mammary epithelial cells from the toxic effects of these minerals by storing them intraluminally and secreting them into milk. The presence of micellar casein in the MSDV lumen, along with the presence of metal transporters in their membranes, is responsible for this impressive capacity for storing reactive and toxic minerals. Exposing a single mammary gland to lipopolysaccharide challenge induced changes in regulatory proteins stored in the lumen of MSDVs (tissue plasminogen activators, plasminogen and plasmin) and in the activity of xanthine oxidase and alkaline phosphatase attached to the outer membrane of MSDVs. Thus, we have demonstrated that MSDVs are under the regulation of the nucleus and respond to extracellular signals. © The Royal Society of Chemistry 2015.
Scientific Publication
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