נגישות
menu      
חיפוש מתקדם
תחביר
חפש...
הספר "אוצר וולקני"
אודות
תנאי שימוש
ניהול
קהילה:
אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
Use of the polymerase chain reaction to clone the potato leafroll virus coat protein gene directly from the total RNA of infected plants
Year:
1995
Source of publication :
Potato Research
Authors :
רוזנר, אריה
;
.
Volume :
38
Co-Authors:
Faccioli, G., Istituto di Patologia Vegetale, Università degli Studi, Bologna, 40126, Italy
Rosner, A., Virology Department, The Volcani Center, Bet-Dagan, 50250, Israel
Forni, M., Istituto di Patologia Vegetale, Università degli Studi, Bologna, 40126, Italy
Facilitators :
From page:
211
To page:
218
(
Total pages:
8
)
Abstract:
The potato leafroll virus (PLRV) coat protein (CP) gene was directly cloned from the total RNA extracted from virus-infected plants. First strand cDNA synthesis was not necessarily specific; it was equally efficient using either random or CP-specific primers. The viral sequence encoding the coat protein was specifically amplified from the total population of cDNA molecules by polymerase chain reaction (PCR), using specific primers bordering the CP gene. The unique amplified product thus obtained was cloned blunt-end into the pT7T318U plasmid vector, and the authenticity of the cloned gene verified by sequence analysis. This cloning strategy obviates the need for virus purification. Sequence comparison of the CP gene of the Italian isolate and those of five other PLRV isolates revealed a close similarity to the three European and the Canadian isolates, and a more distant relationship with the Australian one. © 1995 Kluwer Academic Publishers.
Note:
Related Files :
PCR. DNA sequencing
reverse transcription
Solanum tuberosum
עוד תגיות
תוכן קשור
More details
DOI :
10.1007/BF02357934
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
28108
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:36
You may also be interested in
Scientific Publication
Use of the polymerase chain reaction to clone the potato leafroll virus coat protein gene directly from the total RNA of infected plants
38
Faccioli, G., Istituto di Patologia Vegetale, Università degli Studi, Bologna, 40126, Italy
Rosner, A., Virology Department, The Volcani Center, Bet-Dagan, 50250, Israel
Forni, M., Istituto di Patologia Vegetale, Università degli Studi, Bologna, 40126, Italy
Use of the polymerase chain reaction to clone the potato leafroll virus coat protein gene directly from the total RNA of infected plants
The potato leafroll virus (PLRV) coat protein (CP) gene was directly cloned from the total RNA extracted from virus-infected plants. First strand cDNA synthesis was not necessarily specific; it was equally efficient using either random or CP-specific primers. The viral sequence encoding the coat protein was specifically amplified from the total population of cDNA molecules by polymerase chain reaction (PCR), using specific primers bordering the CP gene. The unique amplified product thus obtained was cloned blunt-end into the pT7T318U plasmid vector, and the authenticity of the cloned gene verified by sequence analysis. This cloning strategy obviates the need for virus purification. Sequence comparison of the CP gene of the Italian isolate and those of five other PLRV isolates revealed a close similarity to the three European and the Canadian isolates, and a more distant relationship with the Australian one. © 1995 Kluwer Academic Publishers.
Scientific Publication
You may also be interested in