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פותח על ידי קלירמאש פתרונות בע"מ -
Persistence of Salmonella enterica during dehydration and subsequent cold storage
Year:
2012
Authors :
גרוזדב, נדיה
;
.
סלע, שלמה
;
.
פינטו, רבקה
;
.
Volume :
32
Co-Authors:
Gruzdev, N., Microbial Food-Safety Research Unit, Department of Food Quality and Safety, Institute for Postharvest and Food Sciences, Agricultural Research Organization (ARO), The Volcani Center, POB 6, Beth-Dagan 50250, Israel, Department of Biochemistry and Food Science, Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University, Rehovot, Israel
Pinto, R., Microbial Food-Safety Research Unit, Department of Food Quality and Safety, Institute for Postharvest and Food Sciences, Agricultural Research Organization (ARO), The Volcani Center, POB 6, Beth-Dagan 50250, Israel
Sela Saldinger, S., Microbial Food-Safety Research Unit, Department of Food Quality and Safety, Institute for Postharvest and Food Sciences, Agricultural Research Organization (ARO), The Volcani Center, POB 6, Beth-Dagan 50250, Israel
Facilitators :
From page:
415
To page:
422
(
Total pages:
8
)
Abstract:
Despite the fact that Salmonella enterica serotype Typhimurium SL 1344 has served as a model pathogen in many studies, information regarding its desiccation response is still scarce. In this study, we investigated environmental conditions that affect Salmonella survival following dehydration and subsequent cold storage, using a 96-well polystyrene plate model. The SL 1344 strain exhibited high survival compared with other Typhimurium isolates and S. enterica serotypes. Further characterization of desiccation tolerance in this strain revealed that temperature, stationary-phase of growth, solid medium, and the presence of increasing NaCl concentrations (0.5-5.0%) in the growth medium enhanced desiccation tolerance. Dehydration at basic pHs (8-10), or in trehalose, sucrose, but not in glycine-betaine, improved bacterial persistence. Dehydrated Salmonella survived over 100 weeks at 4 °C with a ∼5-log reduction in numbers. However, viability staining revealed only a ∼50% reduction in viable cells, suggesting bacterial transition into a viable-but-not-cultivable state (VBNC). Addition of chloramphenicol reduced bacterial survival implying that adaptation to desiccation stress requires de-novo protein synthesis. Consistent with this finding, shortening the dehydration time resulted in lower survival. This study emphasizes the impact of environmental conditions on the fate of dried Salmonella in the food chain and highlights the potential transition of the pathogen to the VBNC state. © 2012 Elsevier Ltd.
Note:
Related Files :
Animal
Animals
Bird Diseases
chemistry
cold storage
Growth, Development and Aging
Microbiology
Salmonella
עוד תגיות
תוכן קשור
More details
DOI :
10.1016/j.fm.2012.08.003
Article number:
0
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
28121
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:36
Scientific Publication
Persistence of Salmonella enterica during dehydration and subsequent cold storage
32
Gruzdev, N., Microbial Food-Safety Research Unit, Department of Food Quality and Safety, Institute for Postharvest and Food Sciences, Agricultural Research Organization (ARO), The Volcani Center, POB 6, Beth-Dagan 50250, Israel, Department of Biochemistry and Food Science, Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University, Rehovot, Israel
Pinto, R., Microbial Food-Safety Research Unit, Department of Food Quality and Safety, Institute for Postharvest and Food Sciences, Agricultural Research Organization (ARO), The Volcani Center, POB 6, Beth-Dagan 50250, Israel
Sela Saldinger, S., Microbial Food-Safety Research Unit, Department of Food Quality and Safety, Institute for Postharvest and Food Sciences, Agricultural Research Organization (ARO), The Volcani Center, POB 6, Beth-Dagan 50250, Israel
Persistence of Salmonella enterica during dehydration and subsequent cold storage
Despite the fact that Salmonella enterica serotype Typhimurium SL 1344 has served as a model pathogen in many studies, information regarding its desiccation response is still scarce. In this study, we investigated environmental conditions that affect Salmonella survival following dehydration and subsequent cold storage, using a 96-well polystyrene plate model. The SL 1344 strain exhibited high survival compared with other Typhimurium isolates and S. enterica serotypes. Further characterization of desiccation tolerance in this strain revealed that temperature, stationary-phase of growth, solid medium, and the presence of increasing NaCl concentrations (0.5-5.0%) in the growth medium enhanced desiccation tolerance. Dehydration at basic pHs (8-10), or in trehalose, sucrose, but not in glycine-betaine, improved bacterial persistence. Dehydrated Salmonella survived over 100 weeks at 4 °C with a ∼5-log reduction in numbers. However, viability staining revealed only a ∼50% reduction in viable cells, suggesting bacterial transition into a viable-but-not-cultivable state (VBNC). Addition of chloramphenicol reduced bacterial survival implying that adaptation to desiccation stress requires de-novo protein synthesis. Consistent with this finding, shortening the dehydration time resulted in lower survival. This study emphasizes the impact of environmental conditions on the fate of dried Salmonella in the food chain and highlights the potential transition of the pathogen to the VBNC state. © 2012 Elsevier Ltd.
Scientific Publication
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